Identification Pseudomonas aeruginosa by 16s rRNA gene for Differentiation from Other Pseudomonas Species that isolated from Patients and environment Mohammed E. Altaai|Ismail H.Aziz|Abeer A. Marhoon

Main Article Content

Baghdad Science Journal

Abstract

Pseudomonas aeruginosa is a common and major opportunistic human pathogen, its causes many and dangersinfectious diseases due to death in some timesex: cystic fibrosis , wounds inflammation , burns inflammation , urinary tract infection , other many infections otitis external , Endocarditis , nosocomial infection and also causes other blood infections (Bacteremia). thereforebecomes founding fast and exact identification of P. aeruginosafrom samples culture very important.However, identification of this species may be problematic due to the marked phenotypic variabilitydemonstrated by samples isolates and the presence of other closely related species. To facilitate species identification, we used 16S ribosomal DNA(rRNA) sequence data toidentify genus- and species-specific 16S rRNA signature sequences,its account a stable part of the geneticcode. Based on these sequences we designed simple, rapid, and accurate PCR assays that allow the differentiation of P. aeruginosa from Pseudomonas species and other pathogen genus ,also this test considered as the most specific than the other diagnostic tests like API (20) which give 70% while the 16SrRNA test give more than 90 %.

Downloads

Download data is not yet available.

Article Details

How to Cite
1.
Journal BS. Identification Pseudomonas aeruginosa by 16s rRNA gene for Differentiation from Other Pseudomonas Species that isolated from Patients and environment: Mohammed E. Altaai|Ismail H.Aziz|Abeer A. Marhoon. Baghdad Sci.J [Internet]. 2014Jun.1 [cited 2021Aug.4];11(2):1028-34. Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2726
Section
article