Influence of the Different Carbon and Nitrogen Sources on the Production of Biodiesel by Oleaginous Fungi Aspergillus terreus, Aspergillus fumigatus

Main Article Content

Shatha Ali Shafiq
Afrah Fahad Abdulkareem
Farha A. Ali Shafi

Abstract

In the present study, the growth and total lipid contents of two oleaginous fungal isolates Aspergillus terreus, Aspergillus fumigatus were compared in different nitrogen and organic carbon sources.  Artificially the fungi were cultured on media consisting of various mono- or di- or polysaccharides and  peptone or yeast extract as elementary sources for carbon and nitrogen, respectively. Media containing sucrose /yeast extract or glucose/ yeast extract were the most effective for lipid production from fungal, during  two weeks  incubation period, the highest biomass of dry weight was (19.6 , 18.8) g / L , (25.8 , 30.5) g /L  and  lipid yield (1, 0.97 )g/L, (0.65, 0.65) g/ L for two isolates Aspergillus terreus and Aspergillus fumigatus  respectively then followed maltose/ yeast extract then  starch / yeast extract .Analysis of fatty acid produced by two fungal isolates  using Gas Chromatography  showed the existence of a wide range of fatty acids, these fatty acids were together saturated and unsaturated. The results revealed that the unevenness in the fatty acids composition mainly depends on the type of carbon and nitrogen sources. The existence of saturated and mono saturated fatty acids in A.fumigatus, A. terreus prove that they are  good candidate for biodiesel production.


The analysis of fatty acid from the fungal growth showed the existence of a wide range of fatty acids   Palmitic ,Oleic acids , stearic acid and linoleic acid     together saturated and unsaturated. The results revealed that the unevenness in the fatty acid composition mainly depends on the type  of carbon  and nitrogen sources. The presence of saturated and mono saturated fatty acid in  A.fumigatus , A. terreus prove that it is a good candidate for biodiesel production.

Downloads

Download data is not yet available.

Article Details

How to Cite
1.
Shafiq SA, Abdulkareem AF, Shafi FAA. Influence of the Different Carbon and Nitrogen Sources on the Production of Biodiesel by Oleaginous Fungi Aspergillus terreus, Aspergillus fumigatus. Baghdad Sci.J [Internet]. 2021Jun.1 [cited 2021Jul.28];18(2):0225. Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/2945
Section
article

References

Li Q, Du W, Liu D. Perspectives of microbial oils for biodiesel production. Appl Microbiol Biotechnol. 2008. 80, 749-756. DOI 10.1007/s00253-008-1625-9

aakob Z, Narayanan BN, Padikkaparambil S. A review on the oxidation stability of biodiesel. Renew. Sust. Energ. Rev. 2014 Jul 1;35:136-53. https://doi.org/10.1016/j.rser.2014.03.055

Ochsenreither K, Gluck C, Stressler T, Fischer L, Syldatk C. Production Strategies and Applications of Microbial Single Cell Oils .front Microiol . 2016; 7 : 1539-1547. DOI:10.3389/fmicb.2016.01539

Kosa M, Ragauskas AJ. Lipids from heterotrophic microbes: advances in metabolism research. Trends Biotechnol. 2011 Feb;29(2):53-61.. Available from: 29:53–61. doi: 10.1016/j.tibtech.2010.11.002.

Rossi M, Amaretti A, Raimondi S, Leonardi A. biodiesel-feedstocks and processing technologies. Dr. Margarita Stoytcheva (Ed.). 2011:Nov 9;1-72-4. DOI:10.5772/25864.

Pacheco1 SMV, Júnior AC, Morgado AF, Júnior AF, Amadi OC, Guisán GM, et al. Isolation and Screening of Filamentous Fungi Producing Extracellular Lipase with Potential in Biodiesel Production. AER. 2015: (3) 101-114. http://www.scirp.org/journal/aer

Xia CH, Zhang J, Zhang W, Hu B. A new cultivation method for microbial oil production: cell pelletization and lipid accumulation by Mucor circinelloides . Biotechnol Biofules. 2011. 4:15. https://doi.org/10.1186/1754-6834-4-15

Magdum SS, Gauri PM, Upendra SA, Kalyanraman V. Competence evaluation of mycodiesel production by oleaginous fungal strains :Mucor circinelloids and Gliocladium roseum. IJEE. 2015. 6(4): 377-382.

Kikukawa H, Sakuradani E, Ando A, Shimizu S, Ogawa J. Arachidonic acid production by the oleaginous fungus Mortierella alpina 1S-4 . J. Adv. Res. 2018:Vol(11): 15-22. https://doi.org/10.1016/j.jare.2018.02.003

Somasekhar D, Venkateshwaran G, Sambaiah K, Lokesh BR. .Effect of culture conditions on lipid and gamma-linoleic acid production by mucoraceous fungi. Pro. Biochem. 2003. 38:1719-1724.

Shafiq ShA. Biodiesel production by oleaginous fungi before and after exposing of U.V. light. Int J Chem Tech Res. 2017; 10 (12): 357-363.

Lai LST, Pan CC, Tzeng BK. The Influence of medium design on lovastatin production by Aspergillus terreus in submerged cultures. ProBiochem. 2003 April30; 38(9): 1317-1326.

Shin DY, Cho HU, Utomo JC, Choi YN, Xu X, Park JM. Biodiesel production from Scenedesmus bijuga grown in anaerobically digested food wastewater effluent. Bioresour. Technol.2015;184:215–221. doi:10.1016/j.biortech.2014.10.090. Epub 2014 Oct 25.

Amaretti A, Raimondi S, Sala M, Roncaglia L, De Lucia M, Leonardi A, et al. Single cell oils of the cold-adapted oleaginous yeast Rhodotorula glacialis DBVPG 4785. Microb. Cell Fact. 2010 Sep 23. ; 23(9): 73. doi: 10.1186/1475-2859-9-73

Subhash GV, Mohan SV. Lipid accumulation for biodiesel production by oleaginous fungus Aspergillus awamori: influence of critical factors. fuel. 2014; 116: 509-515. www.elsevier.com/locate/fuel.

Shafiq Sh A, Ali RH. Myco-diesel Production by Oleaginous Fungi. RJPBCS.2017; 8(4):1252-1259. https://www.rjpbcs.com/pdf/2017_8(2)/[151].pdf

Gunstone FD, John LH, Albert JD. The Lipid Handbook with Cd-Rom. 3rd ed. Boca Raton: CRC Press,London:1472. 2007

Papanikolaou S, Komaitis M, Aggelis G. Single cell oil (SCO) production by Mortierellaisabellina grown on high-sugar content media. Bioresour Technol.2004. 95: 287–291. DOI:10.1016/j.biortech.2004.02.016

Wu S, Hu C, Jin G, Zha X, Zhao Z. Phosphate- limitation mediated lipid production Rodosporidium toruloides. Bioresour Technol. 2010;101: 6124–6129. doi: 10.1016/j.biortech.2010.02.111. Epub 2010 Mar 21.