In Vitro Cytotoxicity Study of Pt Nanoparticles Decorated TiO2 Nanotube Array

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Shaymaa Rajab Baqer
Abdulkareem Alsammarraie
Mahasin Alias
Mohammad Al-Halbosiy
Amaal Sadiq


Titanium dioxide nanotubes were synthesized by anodizing Ti sheets in the ethylene glycol solution and were covered in Pt nanoparticles onto the surface of TiO2NTs using electrodeposition method from using five derivatives of Mannich base Pt complexes which have been used as precursor of platinum. The mean size, shape, elemental composition of the titanium dioxide nanotubes and platinum deposited on the template were evaluated by different techniques such as field emission scanning electron microscope (FE-SEM), transmission electron microscopy (TEM), X-ray diffraction pattern (XRD), and energy dispersive X-ray (EDX) technique. From all these analyses, the TiO2NTs prepared and Ptnanoparticles deposited on it were identified. The diagnoses proved that all the Pt nanoparticles have a size less than 50 nm. The MCF-7 cancer cell lines and WRL68 normal cell lines were treated with concentration 800, 400,200,100, 50, 25, 12.5µg\ml of TiO2NTs and Pt\TiO2NTs(1) and (2) for 48hours using MTT assay.IC50 and inhibition rate were calculated. The result shows that the Pt\TiO2NTs have more inhibition effect on cancer cell lines than TiO2NTs array.


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Baqer SR, Alsammarraie A, Alias M, Al-Halbosiy M, Sadiq A. In Vitro Cytotoxicity Study of Pt Nanoparticles Decorated TiO2 Nanotube Array. Baghdad Sci.J [Internet]. 2020Dec.1 [cited 2021Jan.20];17(4):1169. Available from:


Indira K, Mudali U K, Nishimura T , Rajendran N. A review on TiO2nanotubes influence of anodization parameter, formation mechanism, properties, corrosion behavior, and biomedical applications. J. of bio-tribo-corrosion. 2015; 1(4):1-28.

Zhu Q , Hu H , Li G , Zhu C ,Yu Y. TiO2 nanotube arrays grafted with MnO2 nanosheets as high-performance anode for lithium ion battery. Electrochimi. Acta. 2015; 156: 252-260.‏

Roy P, Berger S ,Schmuki P. TiO2 nanotubes .synthesis and applications. Angew. Chem. Int. Edition, 2011; 50(13):2904-2939.

Sheng J, Chen Y ,Tong H, Guo Y. Preparation and photocatalytic properties of KH-550 modified nano-TiO2graphene composites. Rev.Envirron Risk Assess Remediat.2017; 1(2):62-68.

Pablos C, Marugan J ,van Grieken R, Dunlop P, Hamilton J, Dionysiou D, et al. Electrochemical enhancement of photocatalytie disinfection on aligned TiO2 and nitrogen doped TiO2 nanotubes .Mol.2017; 2(704):1-15.

Bahgat M , Farghali A A , Mustafa A F , Khedr M , Mohassab-Ahmed M Y. Electrical magenetic and corrosion resistance properties of TiO2nanotubes filled with NiFe2O4 quantum dots and Ni-Fenanoalloy. Appl.Nanosci. 2013; 3(3):241-249.

Liu Z , Pesic B , Raja K S, Rangaraju R R, Misra M. Hydrogen generation under sunlight by self ordered TiO2nanotube arrays. Int.J.of Hydrogen Energ.2009; 34(8):3250-3257.

Ayal A K, Zainal Z, Lim H N, Talib Z A, Lim Y C, Chang S K , et al. Electrochemical deposition of CdSe-sensitized TiO2nanotube arrays with enhanced photoelectrochemical performance for solar cell application .J. Mater.Sci.:Mater.Electron.2016; 27(5):5204-5210.

Perillo P , Rodríguez D. TiO2 nanotubes membrane flexible sensor for low-temperature H2S detection. Chemosensors. 2016; 4(15): 1-10

Chen X ,Wu N, Zhang G, Feng S, Xu K, Liu W, et al. Functionalized TiO2 nanotubes as three-dimensional support for loading [email protected], facile preparation and enhanced materials for electrochemical sensor.Int.J.Electrochem.Sci.2017;12:593-609.

Oh S, Daraio C, Chen L-H, Pisanic TR, Fiñones RR, Jin S. Significantly accelerated osteoblast cell growth on aligned TiO2 nanotubes. J Biomed Mater Res, Part A. 2006; 78A (1):97–103.

Oliveira W F, Arruda I R., Silva G M., Machado G, Coelho L C, Correia M T. Functionalization of titanium dioxide nanotubes with biomolecules for biomedical applications. Mater Sci Eng: C. 2017; 81: 597-606.‏

Nasr R, Hasanzadeh H, Khaleghian A, Moshtaghian A, Emadi A, Moshfegh S. Induction of apoptosis and inhibition of invasion in gastric cancer cells by titanium dioxide nanoparticles. Oman med.J. 2018; 33(2): 111-117.‏

Khoee M H, Khoee S , Lotfi M. Synthesis of titanium dioxide nanotubes with liposomal covers for carrying and extended release of 5-FU as anticancer drug in the treatment of HeLa cells. Anal. biochem . 2019; 572: 16-24.‏

Zhang H, Sun Y, Tian A, Xue XX, Wang L, Alquhali A, et al. Improved antibacterial activity and biocompatibility on vancomycin-loaded TiO2 nanotubes: in vivo and in vitro studies. Int J Nanomed. 2013; 8:4379-4389.

Yang T, Qian S, Qiao Y, Liu X. Cytocompatibility and antibacterial activity of titania nanotubes incorporated with gold nanoparticles. Colloids Surf. B. 2016; 145:597–606.

Mei S, Wang H, Wang W, Tong L, Pan H, Ruan C, et al. Antibacterial effects and biocompatibility of titanium surfaces with graded silver incorporation in titania nanotubes. Biomaterials. 2014; 35(14):4255–4265.

Bendale Y, Bendale V, Paul S. Evaluation of cytotoxic activity of platinum nanoparticles against normal and cancer cells and its cancer potential through induction of apoptosis. Integr .Med.Res.2017; 6(2):141-148.

Stepanov A L, Golubev A N, Nikitin S I, Osin Y N. Areview on the fabraction and properties of platinum nanoparticles .Rev.Adv.Mater.Sci.2014; 38(2):160-175.

Mohammadi H, Abedi A, Akbarzadeh A ,Mokhtari M J, Shahmabadi H E, Mehrabi M R et al. Evaluation of synthesized platinum nanoparticles on the MCF-7 and HepG-2 cancer cell lines.Int.NanoLett. 2013; 3(28):1-5.

Alias M, Bakir Sh R. Synthesis,Physico-Chemical characterization ,and cytotoxicity assay of Mannich base derivatives with heavy metal ions on RAW264.7 cell line.JGPT.2017;12(9):302-313.

Alias M , Bakir Sh R. Synthesis,spectroscopic characterization and in vitro cytotoxicity assay of morpholine Mannich base derivatives of benzimidazole with some heavy metals .ANJS.2018; 21(3):50-60.

Freshney R I. Culture of animal cells: a manual of basic technique and specialized applications. John Wiley & Sons; 2015.‏

nanotube arrays modified with nanoparticles of platinum group metals (Pt, Pd, Ru): enhancement on photoelectrochemical performance. J. of Nanoparticle Res. 2019; 21(2): 29, doi:10.1007/s11051-018-4443-8.‏

Vera-Jimenez A M, Melgoza-Aleman R M, Valladares-Cisneros M G, Cuevas-Arteaga C. Synthesis and mechanicalelectrochemical characterization of TiO2nanotubular structures obtained at high voltage.J.of. Nanomater. 2015; 2015, Article ID 624073:1-12.

Kittisakmontree P, Pongthawornsakun B, Yoshida H, Fujita S I, Arai M , Panpranot J.The liquid phase hydrogenation of I-heptyne over Pd-AuTiO2catalysts prepared by the combination of incipient wetness impregnation and deposition-precipitation .J.Catal. 2013; 297: 155-164.

Tsai W B, Kao J Y, Wu T M ,Cheng W T.Dispersion of titanium oxide nanoparticles in aqueous solution with anionic stabilizer via ultrasonic wave. J.Nanoparticles .2016;Article ID,6539581.

Chellappa M, Anjaneyulu U, Manivasagam G, Vijayalakshmi U. Preparation and evaluation of the cytotoxic nature of TiO2 nanoparticles by direct contact method. Int. J. Nanomed. 2015; 10 (Suppl 1: Challenges in bio.res.: 31–41.

Rahimnejad S, Torbati M B. Synthesis of Hydroxyapatite/Ag/TiO2 Nanotubes and Evaluation of Their Anticancer Activity on Breast Cancer Cell Line MCF-7. JCHR .2016; 6(3):1–10.

Waters DJ, Shen S, Glickman LT, Cooley DM, Bostwick DG, Qian J, et al. Prostate cancer risk and DNA damage: translational significance of selenium supplementation in a canine model. Carcinogenesis. 2005 Apr 7;26(7):1256-62.

Latha T S, Reddy M C, Muthukonda S, Srikanth V V, Lomada D.In vitro and in vivo evaluation of anti-cancer activity :shape-dependent properties of TiO2nanostructures.Materi Sci Eng C.2017;78:969-977.