A Prevalence study of Entamoeba spp. in Basrah Province using Different Detection Methods
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Abstract
This study aims to determine the prevalence of Entamoeba histolytica, Entamoeba dispar and
Entamoeba moshkovskii by three methods of diagnosis (microscopic examination, cultivation and PCR) that
were compared to obtain an accurate diagnosis of Entamoeba spp. during amoebiasis. Total (n=150) stool
samples related to patients were (n = 100) and healthy controls (n= 50). Clinically diagnosed stool samples
(n=100) were collected from patients attending the consultant clinics of different hospitals in Basrah during
the period from January 2018 to January 2019. The results showed that 60% of collected samples were
positive in a direct microscopic examination. All samples were cultivated on different media; the Brain heart
infusion agar showed high efficiency and was the most suitable in cultivating the parasite. Data and results of
molecular study were indicated by DNA extraction from stool samples and used in PCR technique with
specific primers. This study identifies different infection percentage for the three species. The highest
infection in Basrah patients was Entamoeba moshkovskii 15% followed by Entamoeba dispar 10% and
Entamoeba histolytica, which was 5%.
Received 9/9/2019
Accepted 5/1/2020
Published Online First 30/4/2021
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
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References
Watanabe K, Petri W A .Molecular biology research
to benefit patients with Entamoeba histolytica
infection .J Mol Microbiol. 2015; 98(2):208-217.
Rojas L, Moran P, Valadez A, Gómez A, Gonzalez E,
Hernández E, et al. Entamoeba histolytica and
Entamoeba dispar infection in Mexican school
children: genotyping and phylogenetic relationship.
BMC infectious diseases. 2016; 16 (1): 485.
Soares NM, Azevedo HC, Pacheco FT, de Souza JN,
Del-Rei RP, Teixeira MC, et al. A Cross-Sectional
Study of Entamoeba histolytica/dispar/moshkovskii
Complex in Salvador, Bahia, Brazil. BioMed research
international. 2019 Jul 24;2019:1-7.
López-López, P, Martínez-López M C, Boldo-León
X M, Hernández-Díaz Y, González-Castro T B,
Tovilla-Zárate C A, et al .Detection and
differentiation of Entamoeba histolytica and
Entamoeba dispar in clinical samples through PCR-
denaturing gradient gel electrophoresis. Braz J Med
Biol Res. 2017; 50 (4): 1-7.
Bahrami F, Haghighi A, Zamini G, Khademerfan M.
Differential detection of Entamoeba histolytica ,
Entamoeba dispar and Entamoeba moshkovskii in
faecal samples using nested multiplex PCR in west of
Iran .J Epidemiol Infect . 2019; 147(96):1-12.
Ngui R , Angal L, Fakhrurrazi S A , Lian Y L , Ling
L Y , Ibrahim J ,et al. Differentiating Entamoeba
histolytica, Entamoeba dispar and Entamoeba
moshkovskii using nested polymerase chain reaction
(PCR) in rural communities in Malaysia .J Parasit
Vectors . 2012; 5(187):1-12.
Fotedar R , Stark D , Marriott D , Ellis J. Entamoeba
moshkovskii infection in Sydney , Australia . Eur J
Clin Microbiol and Infect. Dis. 2008; 27:133-137.
Al-Yaquob A J.Diagnostic study on the causative
agent of amoebiasis by PCR technique and ability of
culturing it in Basrahh province. MSc Thesis, College
of Education. University of Basrah; 2008.
Al-Abodi H R. Phylogenetic sequenceing for species
Entamoeba histolytica, E.dispar, E. moshkovaskii in
Al-Qadisiya Province. PhD dissertation. College of
Education .University of Al-Qadisiya; 2015.
Garcia LS, Arrowood M, Kokoskin E, Paltridge GP,
Pillai DR, Procop GW, et al. Laboratory diagnosis of
parasites from the gastrointestinal tract. Clinical
microbiology reviews. 2018; 31(1):e00025-1.
Clark C G, Diamond L S. Methods for cultivation of
luminal protists of clinical importance. J Clin
Microbiol Rev. 2002; 15(3):329-341.
Haida Z, Hakiman M. A comprehensive review on
the determination of enzymatic assay and
nonenzymatic antioxidant activities. J Food science &
nutrition. 2019; 7(5): 1555-1563.
Elnazeer A M, Elmalik K H, Elshikh A A
.Isolation,excystation and In Vitro culture of Giardia
spp. from fecal samples of suspected patients in
RPMI media . Europan Academic Research. 2016;
(10):10670-10679.
Hamzah Z, Petmitr S, Mungthin M, Leelayoova S.
Differential detection of E.histolytica, E.dispar and E.
moshkovskii by single-round PCR assay. J Clin
Microbiol. 2006; 4:3496-3200.
Roudsari B, McKinney C, Moore D, Jarvik J.
Sensitivity and specificity: imperfect predictors of
guideline utility in radiology. Br J Radiol . 2011; 84
(999): 216-20.
Al-Damerchi A T, Al-Ebrahimi H N. Detection of
major virulence factor of Entamoeba histolytica by
using PCR technique. QMJ. 2016; 12(21):36-45.
Kadir M A, El-Yassin S T, Ali A M. Detection of
Entamoeba histolytica and Giardia lamblia in
children with diarrhea in Tikrit city. Tikrit .J. Pure
Science. 2018; 23(6):57-64.
Al-Kaeebi S R, Al-Difaie R S. Use PCR conventional
for detecting AP and PLA virulence factors of
Entamoeba histolytica in patients stool samples in Al-
Qadisiyah Province. J Wassit Sci Medicine. 2016;
(4):102-110.
Fletcher S M ,Stark D , Harkness J ,Ellis J. Enteric
Protozoa in the Developed World: a Public Health
perspective. Clin Microbiol Rev. 2012; 25 (3): 420-
Nakada-Tsukui K, Nozaki T. Immune response of
amebiasis and immune evasion by Entamoeba
histolytica. Front Immunol . 2016; 7 (175):1-13.
Khan S, Ahmed S, Serajuddin M, Saifullah M K.
Variation in seasonal prevalence and intensity of
progenetic metacercariae of Clinostomum
complanatum infection in Trichogaster fasciatus fish.
Beni-Suef University J. Basic Appl. Sci. 2018; 7:
–316.
Open Access Baghdad Science Journal P-ISSN: 2078-8665
, 18(4): 1163-1172 E-ISSN: 2411-7986
Al- Wahid ZH. Genetic study of pathogenic bacterial
strains from water sources and diarrheal in Basrah
governorate. MSc. Thesis. College of Science.
University of Basrah; 2018.
Ximénez C, González E, Nieves M, Magaña U,
Morán P, Gudiño-Zayas M, et al. Differential
expression of pathogenic genes of Entamoeba
histolytica vs E. dispar in a model of infection using
human liver tissue explants. J PloS one.2017;
(8):1-14
Long KZ, Rosado JL, Montoya Y, de Lourdes Solano
M, Hertzmark E, DuPont HL, Santos JI. Effect of
vitamin A and zinc supplementation on
gastrointestinal parasitic infections among Mexican
children. Pediatrics. 2007 Oct 1;120(4):e846-55.
Berne S, Komel R. Benzoic acid derivatives with
improved antifungal activity: Design,synthesis ,
structure-activity relationship (SAR) and GYP53
docking studies. Bioorg Med Chem. 2015;
(15):4264-4276.
Abu-Mejdad N M, Al-Hilfy A A. Biochemical study
for evaluated three culture media to cultivation
Neagleria fowleri. Int J Innov Res Sci Eng Technol.
; 5(3): 4140-4146.
Balthazar C F , Pimentel T C, Ferrão L L, Almada C
N, Santillo A, Albenzio M, et al.Sheep milk:
Physicochemical characteristics and relevance for
functional food development. Compr Rev Food Sci
F. 2017; 16 (2): 247-262.
Adeyemi K D, Sabow A B, Aghwan Z A, Ebrahimi
M, Samsudin A A, Alimon A R, et al .Serum fatty
acids, biochemical indices and antioxidant status in
goats fed canola oil and palm oil blend. JAST. 2016;
(6): 1-11.
Zaefarian F, Abdollahi M R, Cowieson A, Ravindran,
V. Avian liver: The forgotten organ. Animals. 2019;
(63): 1-23.
Serrano-Luna J, Gutierrez-Meza M, Mejia-Zepeda R,
Galindo-Gomez S .Effect of phosphotidylcholine -
cholestrol liposome of Entamoeba histolytica
virulence. Can J Microbiol. 2010; 56:987-995.
Dinoop K P, Parija S C, Mandal J, Swaminathan R
P.Comparison of nested-multiplex, Taqman & SYBR
Green real-time PCR in diagnosis of amoebic liver
abscess in a tertiary health care institute in India.
IJMR. 2016; 143(1): 49-56
Sidstedt M, Steffen C R, Kiesler K M, Vallone P M.
The impact of common PCR inhibitors on forensic
MPS analysis. Forensic Sci. Int.
Genetics.2019; 40:182-191.