A Prevalence study of Entamoeba spp. in Basrah Province using Different Detection Methods

Main Article Content

Athraa Abd-ulameer Al-Hilfy
Maha Khalil Al-Malak
Muslim Abdulrahman Al-Tomah

Abstract

This study aims to determine the prevalence of Entamoeba histolytica, Entamoeba dispar and
Entamoeba moshkovskii by three methods of diagnosis (microscopic examination, cultivation and PCR) that
were compared to obtain an accurate diagnosis of Entamoeba spp. during amoebiasis. Total (n=150) stool
samples related to patients were (n = 100) and healthy controls (n= 50). Clinically diagnosed stool samples
(n=100) were collected from patients attending the consultant clinics of different hospitals in Basrah during
the period from January 2018 to January 2019. The results showed that 60% of collected samples were
positive in a direct microscopic examination. All samples were cultivated on different media; the Brain heart
infusion agar showed high efficiency and was the most suitable in cultivating the parasite. Data and results of
molecular study were indicated by DNA extraction from stool samples and used in PCR technique with
specific primers. This study identifies different infection percentage for the three species. The highest
infection in Basrah patients was Entamoeba moshkovskii 15% followed by Entamoeba dispar 10% and
Entamoeba histolytica, which was 5%.

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How to Cite
1.
Al-Hilfy AA- ulameer, Al-Malak MK, Al-Tomah MA. A Prevalence study of Entamoeba spp. in Basrah Province using Different Detection Methods. Baghdad Sci.J [Internet]. 2021Dec.1 [cited 2021Dec.4];18(4):1163. Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/4014
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References

Watanabe K, Petri W A .Molecular biology research

to benefit patients with Entamoeba histolytica

infection .J Mol Microbiol. 2015; 98(2):208-217.

Rojas L, Moran P, Valadez A, Gómez A, Gonzalez E,

Hernández E, et al. Entamoeba histolytica and

Entamoeba dispar infection in Mexican school

children: genotyping and phylogenetic relationship.

BMC infectious diseases. 2016; 16 (1): 485.

Soares NM, Azevedo HC, Pacheco FT, de Souza JN,

Del-Rei RP, Teixeira MC, et al. A Cross-Sectional

Study of Entamoeba histolytica/dispar/moshkovskii

Complex in Salvador, Bahia, Brazil. BioMed research

international. 2019 Jul 24;2019:1-7.

López-López, P, Martínez-López M C, Boldo-León

X M, Hernández-Díaz Y, González-Castro T B,

Tovilla-Zárate C A, et al .Detection and

differentiation of Entamoeba histolytica and

Entamoeba dispar in clinical samples through PCR-

denaturing gradient gel electrophoresis. Braz J Med

Biol Res. 2017; 50 (4): 1-7.‏

Bahrami F, Haghighi A, Zamini G, Khademerfan M.

Differential detection of Entamoeba histolytica ,

Entamoeba dispar and Entamoeba moshkovskii in

faecal samples using nested multiplex PCR in west of

Iran .J Epidemiol Infect . 2019; 147(96):1-12.

Ngui R , Angal L, Fakhrurrazi S A , Lian Y L , Ling

L Y , Ibrahim J ,et al. Differentiating Entamoeba

histolytica, Entamoeba dispar and Entamoeba

moshkovskii using nested polymerase chain reaction

(PCR) in rural communities in Malaysia .J Parasit

Vectors . 2012; 5(187):1-12.

Fotedar R , Stark D , Marriott D , Ellis J. Entamoeba

moshkovskii infection in Sydney , Australia . Eur J

Clin Microbiol and Infect. Dis. 2008; 27:133-137.

Al-Yaquob A J.Diagnostic study on the causative

agent of amoebiasis by PCR technique and ability of

culturing it in Basrahh province. MSc Thesis, College

of Education. University of Basrah; 2008.

Al-Abodi H R. Phylogenetic sequenceing for species

Entamoeba histolytica, E.dispar, E. moshkovaskii in

Al-Qadisiya Province. PhD dissertation. College of

Education .University of Al-Qadisiya; 2015.

Garcia LS, Arrowood M, Kokoskin E, Paltridge GP,

Pillai DR, Procop GW, et al. Laboratory diagnosis of

parasites from the gastrointestinal tract. Clinical

microbiology reviews. 2018; 31(1):e00025-1.

Clark C G, Diamond L S. Methods for cultivation of

luminal protists of clinical importance. J Clin

Microbiol Rev. 2002; 15(3):329-341.

Haida Z, Hakiman M. A comprehensive review on

the determination of enzymatic assay and

nonenzymatic antioxidant activities. J Food science &

nutrition. 2019; 7(5): 1555-1563.‏

Elnazeer A M, Elmalik K H, Elshikh A A

.Isolation,excystation and In Vitro culture of Giardia

spp. from fecal samples of suspected patients in

RPMI media . Europan Academic Research. 2016;

(10):10670-10679.

Hamzah Z, Petmitr S, Mungthin M, Leelayoova S.

Differential detection of E.histolytica, E.dispar and E.

moshkovskii by single-round PCR assay. J Clin

Microbiol. 2006; 4:3496-3200.

Roudsari B, McKinney C, Moore D, Jarvik J.

Sensitivity and specificity: imperfect predictors of

guideline utility in radiology. Br J Radiol . 2011; 84

(999): 216-20.

Al-Damerchi A T, Al-Ebrahimi H N. Detection of

major virulence factor of Entamoeba histolytica by

using PCR technique. QMJ. 2016; 12(21):36-45.

Kadir M A, El-Yassin S T, Ali A M. Detection of

Entamoeba histolytica and Giardia lamblia in

children with diarrhea in Tikrit city. Tikrit .J. Pure

Science. 2018; 23(6):57-64.

Al-Kaeebi S R, Al-Difaie R S. Use PCR conventional

for detecting AP and PLA virulence factors of

Entamoeba histolytica in patients stool samples in Al-

Qadisiyah Province. J Wassit Sci Medicine. 2016;

(4):102-110.

Fletcher S M ,Stark D , Harkness J ,Ellis J. Enteric

Protozoa in the Developed World: a Public Health

perspective. Clin Microbiol Rev. 2012; 25 (3): 420-

Nakada-Tsukui K, Nozaki T. Immune response of

amebiasis and immune evasion by Entamoeba

histolytica. Front Immunol . 2016; 7 (175):1-13.

Khan S, Ahmed S, Serajuddin M, Saifullah M K.

Variation in seasonal prevalence and intensity of

progenetic metacercariae of Clinostomum

complanatum infection in Trichogaster fasciatus fish.

Beni-Suef University J. Basic Appl. Sci. 2018; 7:

–316.

Open Access Baghdad Science Journal P-ISSN: 2078-8665

, 18(4): 1163-1172 E-ISSN: 2411-7986

Al- Wahid ZH. Genetic study of pathogenic bacterial

strains from water sources and diarrheal in Basrah

governorate. MSc. Thesis. College of Science.

University of Basrah; 2018.

Ximénez C, González E, Nieves M, Magaña U,

Morán P, Gudiño-Zayas M, et al. Differential

expression of pathogenic genes of Entamoeba

histolytica vs E. dispar in a model of infection using

human liver tissue explants. J PloS one.2017;

(8):1-14

Long KZ, Rosado JL, Montoya Y, de Lourdes Solano

M, Hertzmark E, DuPont HL, Santos JI. Effect of

vitamin A and zinc supplementation on

gastrointestinal parasitic infections among Mexican

children. Pediatrics. 2007 Oct 1;120(4):e846-55.

Berne S, Komel R. Benzoic acid derivatives with

improved antifungal activity: Design,synthesis ,

structure-activity relationship (SAR) and GYP53

docking studies. Bioorg Med Chem. 2015;

(15):4264-4276.

Abu-Mejdad N M, Al-Hilfy A A. Biochemical study

for evaluated three culture media to cultivation

Neagleria fowleri. Int J Innov Res Sci Eng Technol.

; 5(3): 4140-4146.

Balthazar C F , Pimentel T C, Ferrão L L, Almada C

N, Santillo A, Albenzio M, et al.Sheep milk:

Physicochemical characteristics and relevance for

functional food development. Compr Rev Food Sci

F. 2017; 16 (2): 247-262.‏

Adeyemi K D, Sabow A B, Aghwan Z A, Ebrahimi

M, Samsudin A A, Alimon A R, et al .Serum fatty

acids, biochemical indices and antioxidant status in

goats fed canola oil and palm oil blend. JAST. 2016;

(6): 1-11.

Zaefarian F, Abdollahi M R, Cowieson A, Ravindran,

V. Avian liver: The forgotten organ. Animals. 2019;

(63): 1-23.‏

Serrano-Luna J, Gutierrez-Meza M, Mejia-Zepeda R,

Galindo-Gomez S .Effect of phosphotidylcholine -

cholestrol liposome of Entamoeba histolytica

virulence. Can J Microbiol. 2010; 56:987-995.

Dinoop K P, Parija S C, Mandal J, Swaminathan R

P.Comparison of nested-multiplex, Taqman & SYBR

Green real-time PCR in diagnosis of amoebic liver

abscess in a tertiary health care institute in India.

IJMR. 2016; 143(1): 49-56

Sidstedt M, Steffen C R, Kiesler K M, Vallone P M.

The impact of common PCR inhibitors on forensic

MPS analysis. Forensic Sci. Int.

Genetics.2019; 40:182-191.‏