Study the Efficiency of Two Concentrations from Algae Cladophora glomerata Extract on the Giardia lamblia parasite

Main Article Content

Raghad J. Fayyad
Siham N. Lefta
Dina Yousif Mohammed
Sara A.J. Maliki


Giardia lamblia parasite was isolated from the diarrhea samples of patients with Giardiasis dysentery and was developed in HSP media, four mice groups have been used to find in vivo efficacy of two concentrations (128,256) mg/ml of chlorophorm extracts from Cladophora glomerata algae against Giardia lamblia parasite  as compared with (Flagyl) by measuring several biochemical markers as ( GPT and GOT) enzymes ,sodium ,potassium and iron concentration as well as counting the number of parasitic cysts in each mice groups. The results demonstrate that levels of GPTA GOT enzymes have been decreased in mice treated with algal extract. As for the concentration of the Sodium, Potassium and Iron increased in mice treated with algal extract. The number of the Giardia cyst is also reduced in orally inoculated mice with both concentrations of algal extract as compared with positive control and the Flagyl treated group. In terms of bioactive compounds, GC-Mass results indicate the presence of many phytochemicals with different biologically active properties This study represents the first attempt to use Cladophora glomerata derived from phytochemicals to treat giardiasis in vivo.


Download data is not yet available.

Article Details

How to Cite
Fayyad RJ, Lefta SN, Mohammed DY, Maliki SA. Study the Efficiency of Two Concentrations from Algae Cladophora glomerata Extract on the Giardia lamblia parasite. Baghdad Sci.J [Internet]. 2022Apr.1 [cited 2022Jun.26];19(2):0271. Available from:


Al-Mozan H D, Daoud Y T, Dakhil K M. Intestinal parasitic infection effect on some blood components. J Contemp Med Sci. 2017; 3(9): 159–162.

Elnazeer A M, ELmalik K H, ELshikh A A. Isolation, excystation and in vitro Culture of Giardia-spp from fecal samples of suspected patients in RPMI media. Eur.J. Acad. Res.2016;3(16): 10670- 10689.

Manning G,Reiner DS,LanweatT,Dacre M, Smith A, zhai Y, et al.The minmal kinome Giardia lamblia illuminates early .kinase evolution and unique parasite biology. Genom Biol. 2011;12:66-85.

Khudier SM.Epidemiological study of Bovine Giardiasis in Thiqar province. M.SC.,Thesis; College Of Vet. 2011.Med. University of Basrah.

Chandini SK, Ganesan P, Suresh P, Bhaskar N. Antioxidant properties of methanol extract and its solvent fractions obtained from selected Indian red seaweeds. J Food Sci Technol. 2008; 45:1–13.

Holdt SL,Kraan S. Bioactive compounds in seaweed; functional food applications and legislation. J Appl Phycol. 2011; 23: 543–597.

Auer MT, Tomlinson L M, Higgins S N, Malkin SY, Howell ET, Bootsma H A. Great Lakes Cladophora in the 21st century: same alga-different ecosystem. J Great Lakes Res.2010;36(2):248-255.

Athbi AM, Almayah S H,Khalaf AK. Antiparasitic activity of macroalgae Cladophora crispate against protoscolicies of hydatid cysts compared with albendazol drug. Afr. J. Biotechnol. 2014;13(30):3038-3080.

Dwaish S, Yousif D Y M, Alwan A H, Lefta S N . Anti-Dermatophytes Activity of Macroalgal Extracts (Chara vulgaris) Isolated from Baghdad City-Iraq. J.Global Pharma technol. 2018 ; 10(03): 759-766.

Tanyuksel M, Yilmaz H, Ulukanligil M, Araz E, Cicek M, Koru O ,et al . Comparison of two methods (microscopy and enzyme-linked immunosorbent assay) for the diagnosis of amebiasis. Exp. Parasitol.2005; 110(3): 322-326.

Sheffiled H G, Bjorvatan B. Ultrastructure of the cyst of Giardia lamblia. Am. J. Trop. Med. Hyg. 1997; 26(1):23-30.

Ekhlas H A, Abdel-Hafeez A, Ahmad K , Amany M, Kamal U, Belal S, et al. Anti-Giardia lamblia activity of ginger (Zingiber officinale) extract in an improved modified axenic culture. P U J.2017; 9:7–12.

Wootton IDP .Micro Analysis in medical biochemistry 4th ed:J and Achurchill,London, 1974:59

Fayyad R J,Mohammed-ali AN,Dwaish AS, Al-aboodi AK.cytotoxic effect of methanolic extract of Morenga oleifera leaves on two cancer cell lines in vitro. Biosci.Res. 2019 ; 16(2): 1214-1222.

Pamela Mason. Blood test used to investigate liver thyroid or kidney function and disease. Pharm J. 2004; 272:446-448.

Abraham P, Chacko B, Abraham O, Priscilla R, Usha S, Matha D. Piatform abstract of Diamond Apicov.JApl. 2005.;53:278-280.

Baranwal A, Rav M, Rupa S, Sunit S. Sodium stiboluconate and polymorphic ventricular tachycardia. Indian J Pediatr. 2005;72(3):261-273.

Darlan D M, Ananda F R, Sari M I , Arrasyid N K, Sari D I . Correlation between iron deficiency anemia and intestinal parasitic infection in school-age children in Medan. IOP Conference Series .Earth Environ. Sci, 2018; 125.

Fayyad R J,Mohammed-ali AN,Dwaish AS, Al-aboodi AK.Anticancer activity of spirulina platensis methanolic extracts L20B and MCF7 human cancer cell lines. Plant Archives. 2019 ; 19 (1): 1419-1426

Abbas S K. Study the effect of Cladophora glomerata algae extract on the parasite of Entamoeba histolytica. Pak. J. Biotechnol. 2017; 14 (3): 405-409.

Abbas S K, Lefta S N, Majeed H A . study the effect of Cladophora glumerata Algae on the Trichomonas vaginalis parasite. J. Pharm. Sci & Res. 2019;11(2):519-522.

Randjelovic P, Veljkovic S, Stojiljkovic N, Sokolovic D, Ilic I, Laketic D, et al.The Beneficial Biological Properties of Salicylic Acid. Review article. Acta Fac. Med. Naissensis. 2015;32(4):259-265.

Arora S, Kumer G, Meena S. Gas chromatography-mass spectroscopy analysis root of an economically important plant , Cenchrus ciliaris l. from thar desert, Rajasthan INDIA. Asian J Pharm Clin Res. 2017; 10 (9): 64-69.

Kavitha1 A M, Uduman M.Identification Of Bioactive Components and Its Biological Activities of Abelmoschas moschatus Flower Extrtact - A Gc-Ms Study. J. Applied Chemistry. .2017; 10(11): 19-22.