Isolation and identification of some bacterial isolates from soil contaminated with crude oil and Testing Their Effectiveness

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Nabila Kridi
Mohammed Said Al-Shater
Mohammed Manhal Al Zoubi


Bacterial strains were isolated from oil-contaminated soil, in 2018, these isolates were identified, and with the aim of finding out the ability of these isolates to degrede the oil compounds, the color change of medium which added to it isolates was read by the method of Pacto Bushnell Hans. Then the change in the petroleum compounds was read by gas chromatography, for the most effective isolates.

The nine isolated bacterial showed different degrees of color change, and the isolates (Pseudomonas, Bacillus, Micrococcus) outperformed the color change amount (78, 78, 77) %, respectively, compared to the control, and the three isolates together showed the best color change of 90.7. % Compared to the control, and the results of separating materials by means of gas chromatography showed that there were changes in the nature of the materials present in the crude oil (control: without isolates) compared to those present in the crude oil That disintegrated by means of the three single isolates. The three isolates together showed a greater ability to change the nature of the materials present in the crude oil. Which confirms the effectiveness of each isolate separately and the ability of these isolates together to degerde crude oil.


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Kridi N, Al-Shater MS, Al Zoubi MM. Isolation and identification of some bacterial isolates from soil contaminated with crude oil and Testing Their Effectiveness. Baghdad Sci.J [Internet]. 2021Dec.20 [cited 2022Jan.20];18(4(Suppl.):1476. Available from:


Pete AJ, Bharti B, Benton MG. Nano-enhanced Bioremediation for Oil Spills: A Review. ACS ES&T Eng. 2021 Apr 29;1(6):928-46.

Osuji, LC, Egbuson EJ, Ojinnaka CM. Chemical reclamation of crude-oil-inundated soils from Niger Delta Nigeria. Chem Ecol. 2005. Feb; 21(1) :1– 10

Oyem IL, Oyem IL. Effects of crude oil spillage on soil physico-chemical properties in Ugborodo community. IJMER. 2013;3(6): 3336-3342.

Smith MJ, Flowers TH, Duncan HJ, Alder J. Effects of polycyclic aromatic hydrocarbons on germination and subsequent growth of grasses and legumes in freshly contaminated soil and soil with aged PAHs residues. Environ Pollut. 2006 Jun 1;141(3):519-25.

Ekundayo Fo, Olukunle Of, Ekundayo E A. Biodegradation of Bonnylight crude oil by locally isolated fungi from oil contaminated soils in Akure. Ondo state, Malays J Microbiol .2012 Jan; 8(1): 42-46.

Hidayat A, Tachibana S. Crude oil and n-octadecane degradation under saline conditions by Fusarium sp. F092, J Environ Sci Technol. 2013 Jan; 6(1):29-40.

Srujana K, Khan AB. Isolation and characterisation of polycyclic aromatic hydrocarbon degrading soil microbes from automobile workshop sediments. J Environ Sci Technol 2012; 5(1): 74-83.

Abramovitch RA, Capracotta M. Remediation of waters contaminated with pentachlorophenol. Chemosphere. 2003 Feb 1; 50(7): 955-7.

Akpoveta OV, Egharevba F, Medjor OW. A pilot study on the biodegradation of hydrocarbon and its kinetics on kerosene simulated soil. Int J Environ Sci. 2011; 2(1): 54-67.

Jebril N M T, In Vitro Bioremediation: A Development Process of Cadmium and Mercury Removal by Environmental Biotechnologies of UV-Mutated Escherichia coli K12 and Bacillus subtilis. Baghdad Sci J 2020.17(1): 244-254. Available from: NMT

AL-Ghamdi AY. Investigation the ability of five fungal species to utilize Gasoline as sole carbon source, Egypt. Acad J Biolog Sci, 2011.3: 7 – 12.

Snape I, Riddle M J, Stark J S, Cole C M, King C K, Duquesne S, Gore D B. Management and remediation of contaminated sites at Casey Station, Antarctica. Polar Rec. Jul 2001. 37(202) : 199 – 214.

Achal V, Kumari D, Pan X, Bioremediation of chromium contaminated soil by a brown-rot fungus, Gloeophyllum sepiarium. Res J Microbiol. 2011; Feb 6(2): 166-171.

Sebiomo A, Banjo FM, Ade-Ogunnowo FE, Fagbemi F. Microbial Population, Dehydrogenase and Urease Activities in Soils Polluted with Spent Engine and Diesel Oil. AJSN. 2017 Sep 2017; 4: 56-65.

Marchand C, St-Arnaud M, Hogland W, Bell TH, Hijri M. Petroleum biodegradation capacity of bacteria and fungi isolated from petroleum-contaminated soil. Int Biodeterior Biodegrad. 2017 Jan 1; 116: 48-57.

Akinnibosun FI, Ashegbare A. Isolation and characterization of hydrocarbon-degrading fungi from Ogbe-Ijoh oil creek, Warri, Delta State, Nigeria.A.S.U.O.C. 2018 Jul. 29(2)72-76.

Sebiomo A, Banjo A, Mary F, Eunice A, Tinuade F. Microbial Population, Dehydrogenase and Urease Activities in Soils Polluted with Spent Engine and Diesel Oil,AJSN. 2017; Sep 15; 4: 56-65.

Almamoori A M, Saleh M M, Salman J M. Bioremediation of polycyclic aromatic hydrocarbons polluted soils using augmentation by inoculating with bacteria (Pseudomonas aeruginosa) and fungi (Penicillium expansum). 2017; Mesopo. Environ j, 3: 3 10-24.

Ojewumi EO. Alkaline pre-treatment and enzymatic hydrolysis of waste papers to fermentable sugar. JEE. 2018; 19(1): 211–217, /https://doi. org/10. /12911/22998993/79404

Nwakanma C, Obih EC, Onyia O. Molecular identification of bacteria involved in degradation of crude oil. Niger J Biotechnol. 2016; 31: 1-8.

Kiama CW. Isolation and Characterization of Hydrocarbon Biodegrading Fungi from oil contaminated soils in Thika, Kenya. Master dissertation: Jomo Kenyatta University of Agriculture and Technology; 2015

Khan S R, Ji N K, Kumar R N, Patel J G. In vitro study on assessment of petrol, kerosene and diesel degrading potential of indigenous fungal isolates from different petroleum product effected soils. Int J Recent Res Rev. 2015; 8(1): 8-15.

Das K, Mukherjee AK. Crude petroleum-oil biodegradation efficiency of Bacillus subtilis and Pseudomonas aeruginosa strains isolated from a petroleum-oil contaminated soil from North-East India. Bioresource technology. 2007 May 1; 98(7): 1339-45.

Staley J T, Bryant M.P, Pfennig N, Holt J G. Bergey's Manual of Systematic Bacteriology, Williams & Wilkins. 1989; 3: 1771-1780

Sneath PH, Mair NS, Sharpe ME, Holt JG. Bergey's manual of systematic bacteriology, Williams and Wilkins, Baltimore USA, 1986; 2

Al-Dhabaan FA. Morphological, biochemical and molecular identification of petroleum hydrocarbons biodegradation bacteria isolated from oil polluted soil in Dhahran, Saud Arabia. Saudi J Biol Sci. 2019 Sep; 26(6): 1247-1252. doi: 10.1016/j.sjbs.2018.05.029. Epub 2018 May 31. PMID: 31516354; PMCID: PMC6733695.

Udgire M, Shah N, Jadhav M. Enrichment, isolation and identification of hydrocarbon degrading bacteria. J Int Curr Microbiol Appl Sci. 2015; 4: 708-13.

Owaied Y. The Effects of Some Single Bacterial Isolates and Combination between them on Biodegradation of 2-Kirkuk Crude Oil. Rafidain, JOS. 2008; May 1;19(2):101-115.

Al-Juboory YH. The Biodegradation for Two Kinds of Crude Oils by the Action of Fusarium moniliforme and Aspergillus flavus. Tikrit j pure sci. 2017; 22(1): 9-17