Main Article Content
This study aims to investigate the possible role of circulating microRNA-142-3p (miR-142-3p) in the
development of graves disease (GD) and its association with the antibody directed against thyroid
stimulating hormone receptor (TSHR-Ab) production in patients with GD. Forty patients with positive
TSHR-Ab enrolled in this study were divided ,based on treatment, into (22 untreated (newly diagnosed) and
18 treated patients) and based on family history (30 with positive family history and 10 with negative family
history). In addition to forty healthy subjects with sex and age matching as a control group. The expression
level of circulating miR-142-3p was determined by two steps reverse transcription polymerase chain reaction
(RT-PCR) technique. Results show that there is a significant elevation (p < 0.01) in the expression of miR-
142-3p in the serum of both treated and untreated patients compared with controls and in patients with
positive family history compared with negative family history. While its expression is non-significantly
lower (P > 0.05) in the serum of treated patients compared with untreated ones. It has been found that miR-
142-3p expression was positively correlated with levels of TSHR-Ab, FT3, and FT4. In addition, the miR-
142-3p expression has a good diagnostic accuracy with sensitivity (82%) and specificity (80%). In
conclusion, the differential expression of miR-142-3p between patients and healthy controls appears as a
potential biomarker for diagnosis of GD and the positive correlation of miR-142-3p with TSHR-Ab
suggesting the contribution of this miRNA in the development of GD.
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Zheng L, Zhuang C, Wang X, Ming L. Serum miR-
a, miR-155, and miR-210 as potential markers of
Graves’ disease. J Clin Lab Anal. 2018;32(2):e22266.
Gonzalez-Amaro R, Marazuela M. T regulatory
(Treg) and T helper 17 (Th17) lymphocytes in thyroid
autoimmunity. Endocrine. 2016;52(1):30-8.
Lombardi A, Menconi F, Greenberg D, Concepcion
E, Leo M, Rocchi R, et al. Dissecting the genetic
susceptibility to Graves’ disease in a cohort of
patients of Italian origin. Frontiers in endocrinology.
Mitchell AL, Pearce SHS. 70 - Autoimmune Thyroid
Diseases. In: Rich RR, Fleisher TA, Shearer WT,
Schroeder HW, Frew AJ, Weyand CM, editors.
Clinical Immunology (Fifth Edition). London:
Content Repository Only!; 2019. p. 947-56.e1.
Kahaly GJ, Bartalena L, Hegedüs L, Leenhardt L,
Poppe K, Pearce SH. 2018 European Thyroid
Association Guideline for the Management of
Graves’ Hyperthyroidism. Eur Thyroid J.
Hiratsuka I, Yamada H, Munetsuna E, Hashimoto S,
Itoh M. Circulating MicroRNAs in Graves' Disease in
Relation to Clinical Activity. Thyroid.
Yau TO, Tang CM, Harriss EK, Dickins B,
Polytarchou C. Faecal microRNAs as a non-invasive
tool in the diagnosis of colonic adenomas and
Open Access Baghdad Science Journal P-ISSN: 2078-8665
, 18(4): 1133-1139 E-ISSN: 2411-7986
colorectal cancer: A meta-analysis. Sci Rep.
Garo LP, Murugaiyan G. Contribution of MicroRNAs
to autoimmune diseases. Cell Mol Life Sci.
Panagal M, Biruntha M, Vidhyavathi RM,
Sivagurunathan P, Senthilkumar SR, Sekar D.
Dissecting the role of miR-21 in different types of
stroke. Gene. 2019;681:69-72.
Abdul-Maksoud RS, Sediq AM, Kattaia A, Elsayed
W, Ezzeldin N, Abdel Galil SM, et al. Serum miR-
and miR-155 expression levels as novel
biomarkers for rheumatoid arthritis diagnosis. Br J
Biomed Sci. 2017;74(4):209-13.
Schmittgen TD, Livak KJ. Analyzing real-time PCR
data by the comparative CT method. Nature
Zhang L, Masetti G, Colucci G, Salvi M, Covelli D,
Eckstein A, et al. Combining micro-RNA and protein
sequencing to detect robust biomarkers for Graves'
disease and orbitopathy. Sci Rep. 2018;8(1):8386.
Allelein S, Diana T, Ehlers M, Kanitz M, Hermsen D,
Schott M, et al. Comparison of a Bridge
Immunoassay with Two Bioassays for Thyrotropin
Receptor Antibody Detection and Differentiation.
Horm Metab Res. 2019;51(6):341-6.
Diana T, Olivo PD, Kahaly GJ. Thyrotropin receptor
blocking antibodies. Horm Metab Res. 2018
Hesarghatta S A, Abraham P. Measuring TSH
receptor antibody to influence treatment choices in
Graves' disease. J Clin Endocrinol Metab.
Chen X, Zhou Y, Zhou M, Yin Q, Wang S.
Diagnostic Values of Free Triiodothyronine and Free
Thyroxine and the Ratio of Free Triiodothyronine to
Free Thyroxine in Thyrotoxicosis. Int J Endocrinol.
Chen X, Huang F, Qi Y, Zhou M, Yin Q, Peng Y, et
al. Serum and thyroid tissue level of let-7b and their
correlation with TRAb in Graves’ disease. J Transl
Mazzone R, Zwergel C, Artico M, Taurone S, Ralli
M, Greco A, et al. The emerging role of epigenetics
in human autoimmune disorders. Clin Epigenetics.
Wang J, Chen J, Sen S. MicroRNA as Biomarkers
and Diagnostics. J Cell Physiol. 2016;231(1):25-30.
Martinez-Hernandez R, Sampedro-Nunez M,
Serrano-Somavilla A, Ramos-Levi AM, de la Fuente
H, Trivino JC, et al. A MicroRNA Signature for
Evaluation of Risk and Severity of Autoimmune
Thyroid Diseases. J Clin Endocrinol Metab.
Long H, Wang X, Chen Y, Wang L, Zhao M, Lu Q.
Dysregulation of microRNAs in autoimmune
diseases: Pathogenesis, biomarkers and potential
therapeutic targets. Cancer Lett. 2018;428:90-103.
Esmailzadeh S, Mansoori B, Mohammadi A,
Baradaran B. Regulatory roles of micro-RNAs in T
cell autoimmunity. Immunol Invest. 2017;46(8):864-
Sharma S. Immunomodulation: A definitive role of
microRNA-142. Dev Comp Immunol. 2017;77:150-
Liu Y, Song X, Meng S, Jiang M. Downregulated
expression of miR-142-3p in macrophages
contributes to increased IL-6 levels in aged mice. Mol
Liu C, Yang H, Shi W, Wang T, Ruan Q.
MicroRNA-mediated regulation of T helper type
/regulatory T-cell balance in autoimmune disease.
Dudics S, Venkatesha SH, Moudgil KD. The Micro-
RNA Expression Profiles of Autoimmune Arthritis
Reveal Novel Biomarkers of the Disease and
Therapeutic Response. Int J Mol Sci. 2018;19(8):
Al-Heety RA, Al-Hadithi HS, Turki KM. Correlation
of circulating miRNA-146a-5p and let-7b expression with thyroid-stimulating hormone receptor antibody
in patients with graves disease. Gene Reports.
Wang B, Shao X, Song R, Xu D, Zhang JA. The
Emerging Role of Epigenetics in Autoimmune
Thyroid Diseases. Front Immunol. 2017;8:396.
Zhu J, Zhang Y, Zhang W, Zhang W, Fan L, Wang L,
et al. MicroRNA-142-5p contributes to Hashimoto's
thyroiditis by targeting CLDN1. J Transl Med.