In Situ Hybridization for Molecular Detection of Human Papilloma Viral 6 / 11 DNA in Adenoctomized Tissues from A group of Iraqi Pediatric Patients
Main Article Content
Abstract
Among more than 200 different human papilloma viral genotypes, the association of low oncogenic risk-HPV genotypes have been recognized with a variety of oral, oropharyngeal, nasopharyngeal benign tumors as well as non-neoplastic polyposis and papillomas and adenoid hypertrophy. This prospective case- control study aims to determine the rate of DNA detection of HPV genotype 6/11 in nasopharyngeal adeno- tonsillar tissues from a group of patients subjected to adenoctomy for adenoid hypertrophy . A total number of nasopharyngeal adeno-tonsillar tissue specimens from pediatric patients with adenoid hypertrophy were enrolled; 40 nasopharyngeal adeno-tonsillar tissues from patients with adenoid hypertrophy, and 20 normal nasal tissue specimens were obtained from pediatric patients following trimming operations of their inferior nasal turbinates' with unremarkable pathological changes (as an apparently healthy control group). The molecular detection methods for HPV detection were performed by using DNA probes via a recent version of chromogenic in situ hybridization specified for low- risk HPV genotypes. Among total adenoid hypertrophied tissue specimens group, 8 out of 40 were found to contain positive results for DNA of HPV 6 / 11 genotype, constituting 20% of the total screened nasopharyngeal adenotonsillar tissues. No positive– CISH reactions were detected in the control nasal tissues. The statistical analysis of results in this research showed significant difference when compared to the control apparently healthy tissues. The significant rate of low- oncogenic HPV genotypes detection in those adenoid hypertrophied tissues could play, in part, a role in their pathogenesis and / or constituting a herald focus for the spread of such important virally transmitted infection.
Received 11/6/2020
Accepted 1/11/2020
Published Online First 20/7/2021
Article Details
This work is licensed under a Creative Commons Attribution 4.0 International License.
How to Cite
References
Bowers I, Shermetaro C. Adenoiditis. InStatPearls [Internet] 2019 Jun 8. StatPearls Publishing.
Proenca-Modena JL, Paula FE, Buzatto GP, Carenzi LR, Saturno TH, Prates MC, et al. Hypertrophic adenoid is a major infection site of human bocavirus 1.J Clin Microbiol. 2014 Aug 1;52(8):3030-7.
Günel C, Kırdar S, Ömürlü İK, Ağdaş F. Detection of the Epstein–Barr virus, Human Bocavirus and novel KI and KU polyomaviruses in adenotonsillar tissues. Int J Pediatr Otorhinolaryngol. 2015; 79(3):423-427.
Assadian F, Sandström K, Bondeson K, Laurell G, Lidian A, Svensson C, et al. Distribution and Molecular Characterization of Human Adenovirus and Epstein-Barr Virus Infections in Tonsillar Lymphocytes Isolated from Patients Diagnosed with Tonsillar Diseases. PLoS ONE. 2016; 11(5): e0154814.
Aboud K, Tarik G, Massimo T, Véronique D, Gary M. Clifford, Jean Lacau St Guily, Christine Clavel, Silvia Franceschi and Jean‐Damien Combes, Prevalence of human herpesviruses infections in nonmalignant tonsils: The SPLIT study. J Med Virol. 2018; 91, 4: 687-697.
Boda D, Docea AO, Calina D, Ilie MA, Caruntu C, Zurac S, et al. Human papilloma virus: Apprehending the link with carcinogenesis and unveiling new research avenues. Int J Oncol. 2018 Feb 28;52(3):637-55.
Taberna M, Mena M, Pavón MA, Alemany L, Gillison ML, Mesía R. Human papillomavirus-related oropharyngeal cancer. Ann Oncol. 2017 Oct 1;28(10):2386-98.
Kazuhiro K, Kenji H, Natsuko S, Akira H, Hiroyuki T, Tatsuhiko M. A Review of HPV-Related Head and Neck Cancer. J Clin Med. 2018 ; 7(9): 241.
Wojtera M, Paradis J, Husein M, Nichols AC, Barrett JW, Salvadori MI, Strychowsky JE. The prevalence of human papillomavirus in pediatric tonsils: a systematic review of the literature. J Otolaryngol Head Neck Surg. 2018; 47: 8.
Syrjänen S. HPV infections and tonsillar carcinoma. J Clin Pathol. 2004 57(5): 449–455.
Ali SHM, Al-Hijazi AY, Khashman BM. P53-tumor suppressor gene overexpression in human papilloma virus-infected patients with oral squamous cell carcinoma. JBCD 2011; 23(sp. issue):70-76.
Ali SHM, Hussien IA, Hussein TA, Kamal MS. Nuclear in Situ Hybridization of High –Risk and Low-Risk Human Papilloma Virus DNA in Tissues from a Group of Iraqi Patients with Sino Nasal and Nasopharyngeal Benign and Malignant Tumors. J. Glob. Pharma Technol. 2017; 10(9):362-370.
Palmer E, Newcombe RG, Green AC, Kelly C, Noel Gill O, Hall G, et al. Human papillomavirus infection is rare in nonmalignant tonsil tissue in the UK: implications for tonsil cancer precursor lesions. Int J Cancer. 2014;135(10):2437–2443.
Sun YF, Wu YD, Wu L, Jiang JJ, Gao R, Xu B, Chen XW, Zhao ZY. [Detection of human papillomavirus in the upper respiratory tract in children without recurrent respiratory papillomatosis]. Zhonghua Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2012 Dec;47(12):974-7. Chinese. PMID: 23328034.
Venuti A, Paolini F. HPV detection methods in head and neck cancer. Head Neck Pathol. 2012;6(suppl 1):S63–S74.
Jordan RC, Lingen MW, Perez-Ordonez B, He X, Pickard R, Koluder M, et al. Validation of methods for oropharyngeal cancer HPV status determination in United States cooperative group trials. Am J Surg Pathol. 2012;36(7):945–954.
Syrjänen K, Syrjänen S. Papillomavirus infections in human pathology. London: Wiley, 2000.
Rosen BJ, Walter L, Gilman RH, Cabrerra L, Gravitt PE, Marks MA. Prevalence and correlates of oral human papillomavirus infection among healthy males and females in Lima, Peru. Sex Transm Infect. 2016;92:149–54.
Brandsma JL, Abramson AL. Association of papillomavirus with cancers of the head and neck. Arch Otolaryngol Head Neck Surg. 1989;115:621-5.
Bercovich JA, Centeno CR, Aguilar OG, Grinstein S, Kahn T. Presence and integration of human papillomavirus type 6 in a tonsillar carcinoma. J Gen Virol. 1991;72:2569–72.
Schwartz SM, Daling JR, Madeleine MM, Doody DR, Fitzgibbons ED, Wipf GC, et al. Oral cancer risk in relation to sexual history and evidence of human papillomavirus infection. .J Natl Cancer Inst 1998;90:1626–36.
Badaracco G, Venuti A, Morello R, Muller A, Marcante ML. Human papillomavirus in head and neck carcinomas: prevalence, physical status and relationship with clinical/pathological parameters. Anticancer Res. 2000;20(2B):1301.
Rieth KKS, Gill SR, Lott-Limbach AA, Merkley MA, Botero N, Allen PD, et al. Prevalence of high-risk human papillomavirus in tonsil tissue in healthy adults and colocalization in biofilm of tonsillar crypts. JAMA Otolaryngol Head Neck Surg. 2018;144:231–7.
Lupato V, Holzinger D, Höfler D, Menegaldo A, Giorgi Rossi P, Del Mistro A, et al. Prevalence and determinants of oral human papillomavirus infection in 500 young adults from Italy. PLoS One.2017;12:e0170091.
Zlobec, R. S. ; René, P .M.;Carolyn, C.; Compton, ;Alessandro Lugl and Jeremy, R . J. Scoring of p53, VEGF, Bcl-2 and APAF-1 immunohistochemistry and interobserver reliability in colorectal cancer. Modern Pathology 2006; 19: 1236–1242.