Main Article Content
Biodiesel production from microalgae depends on the biomass and lipid production. Both biomass and lipid accumulation is controlled by several factors. The effect of various culture media (BG11, BBM, and Urea), nutrients stress [nitrogen (N), phosphorous (P), magnesium (Mg) and carbonate (CO3)] and gamma (γ) radiation on the growth and lipid accumulation of Dictyochloropsis splendida were investigated. The highest biomass and lipid yield of D. splendida were achieved on BG11 medium. Cultivation of D. splendida in a medium containing 3000 mg L−1 N, or 160 mg L−1 P, or 113 mg L−1 Mg, or 20 mg L-1 CO3, led to enhanced growth rate. While under the low concentrations of nutrients caused a marked increase in the lipid content. Cultures exposure to 25 Gy of γ-rays, led to an increase in lipid content up to 18.26 ± 0.81 %. Lipid profile showed the maximum presence of saturated fatty acids (SFAs, 63.33%), and unsaturated fatty acids (UFAs, 37.02%). Fatty acids (FAs) recorded the predominance of C16:0, C18:2, C15:0 and C16:1, which strongly proved D. splendida is a promising feedstock for biodiesel production.
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Mofijur M, Rasul M G, Hassan N M , Nabi M N. Recent development in the production of third generation biodiesel from microalgae. Energy Procedia 2019; 156: 53-58.
Shomal R, Hisham H, Mlhem A, Hassan R, Al-Zuhair S. Simultaneous extraction–reaction process for biodiesel production from microalgae. Energy Rep. 2019; 5: 37-40.
Singh D, Sharma D, Soni SL, Sharma S, Sharma PK, Jhalani A. A review on feedstocks, production processes, and yield for different generations of biodiesel. Fuel. 2020 15;262:116553.
Anwar F, Rashid U, Ashraf M, Nadeem M. Okra (Hibiscus esculentus) seed oil for biodiesel production. Appl. Energy 2010; 87: 779-785.
Chang A, Pan JH, Lai NC, Tsai MC, Mochizuki T, Toba M, et al. Efficient simultaneous esterification/transesterification of non-edible Jatropha oil for biodiesel fuel production by template-free synthesized nanoporous titanosilicates. Catalysis Today. 2019 12.
Yin Z, Zhu L, Li S, Hu T, Chu R, Mo F, et al. A comprehensive review on cultivation and harvesting of microalgae for biodiesel production: environmental pollution control and future directions. Bioresour. Technol. 2020; 122804.
Chozhavendhan S, Singh MV, Fransila B, Kumar RP, Devi GK. A review on influencing parameters of biodiesel production and purification processes. Curr. Opin. Green Sustain. 2020; 1(1):1-6.
Abo-State MA M, Shanab S M M, Ali H E A. Effect of nutrients and gamma radiation on growth and lipid accumulation of Chlorella vulgaris for biodiesel production. J. Rad. Res. Appl. Sci. 2019; 12(1): 332-342.
Ali, H E A, El-fayoumy, E A, Rasmy, W E, Soliman, R M, Abdullah, M A. Two-stage cultivation of Chlorella vulgaris using light and salt-stress conditions for simultaneous production of lipid, carotenoids, and antioxidants. J. Appl. Phycol. 2021; 33: 227–239.
Golz A L, Bradshaw C. Gamma radiation induced changes in the biochemical composition of aquatic primary producers and their effect on grazers. Front. Environ. Sci. 2019; 7: 100.
Gomes T, Xie L, Brede D, Lind O C, Solhaug K A, Salbu B, et al . Sensitivity of the green algae Chlamydomonas reinhardtii to gamma radiation: Photosynthetic performance and ROS formation. Aqu. Toxicol. 2017; 183: 1-10.
Stanier RY, Kunisawa R, Mandel M, Cohen-Bazire G. Purification and properties of unicellular blue-green algae (order Chroococcales). Bacteriol. Rev. 1971; 35(2): 171-205.
Bischoff H W, Bold HC. Some soil algae from enchanted rock and related algal species. Austin, Tex.: University of Texas 1963.
Crofcheck C L, Monstross M, Xinyi E, Shea AP, Crocker M, Andrews R. Influence of media composition on the growth rate of Chlorella vulgaris and Scenedesmus acutus utilized for CO2 mitigation. J. Biochem. Technol. 2012; 4: 589-594.
Vidyashankar S, Shankara Murthy V, Venkata Swarnalatha G MD K, Chauhan V, Ravi R, Bansal AK, et al. Characterization of fatty acids and hydrocarbons of chlorophycean microalgae towards their use as biofuel source. Biomass Bioenergy 2015; 77: 75-91.
Bligh EG, Dyer W J. A rapid method of total lipid extraction and purification. Canadian J. Biochem. Physiol. 1959; 37(8): 911-917.
Hempel N, Petrick I, Behrendt F. Biomass productivity and productivity of fatty acids and amino acids of microalgae strains as key characteristics of suitability for biodiesel production. J. Appl. Phycol. 2012; 24(6): 1407-1418.
Yang F, Long L, Sun X, Wu H, Li T, Xiang W. Optimization of medium using response surface methodology for lipid production by Scenedesmus sp. Mar. Drugs 2014; 12(3): 1245-1257.
Christie W W. Preparation of ester derivatives of fatty acids for chromatographic analysis. Adv. lipid Methodol. 1993; 2(69): e111.
Chandra R, Ghosh UK. Effects of various abiotic factors on biomass growth and lipid yield of Chlorella minutissima for sustainable biodiesel production. Environ. Sci. Poll. Res. 2019; 26(4): 3848-3861.
Zhang Q, HongY. Comparison of growth and lipid accumulation properties of two oleaginous microalgae under different nutrient conditions. Front. Environ. Sci. Eng. 2014; 8(5): 703-709.
Bruland KW, Donat JR, Hutchins DA. Interactive influences of bioactive trace metals on biological production in oceanic waters. Limnol. Oceanogr. 1991; 36(8): 1555-1577
Matsudo M, Bezerra R, Sato S, Perego P, Converti A, Carvalho J C. Repeated fed-batch cultivation of Arthrospira (Spirulina) platensis using urea as nitrogen source. Biochem. Eng. J. 2009; 43: 52-57.
Sajjadi B, Chen W-Y, Raman A A A, Ibrahim S. Microalgae lipid and biomass for biofuel production: A comprehensive review on lipid enhancement strategies and their effects on fatty acid composition. Renew. Sustain. Energy Rev. 2018; 97: 200-232.
Ishika T, Moheimani NR, Bahri PA. Sustainable saline microalgae co-cultivation for biofuel production: a critical review. Renew. Sustain. Energy Rev. 2017; 78: 356–68
Rehman ZU, Anal A K. Enhanced lipid and starch productivity of microalga (Chlorococcum sp. TISTR 8583) with N limitation following effective pretreatments for biofuel production. Biotechnol. Rep. 2019; 21: e00298.
Yodsuwan N, Sawayama S, Sirisansaneeyakul S. Effect of N concentration on growth, lipid production and fatty acid profiles of the marine diatom Phaeodactylum tricornutum. J Agric Nat Resour. 2017 1;51:190-7.
Guschina I A, Harwood J L. Algal lipids and effect of the environment on their biochemistry. In M. Kainz, M. T. Brett & M. T. Arts (Eds.), Lipids in Aquatic Ecosystems 2009; (pp. 1-24). New York, NY: Springer New York
Huang B, Marchand J, Thiriet-Rupert S, Carrier G, Saint-Jean B, Lukomska E, et al. Betaine lipid and neutral lipid production under nitrogen or phosphorous limitation in the marine microalga Tisochrysis lutea (Haptophyta). Algal Res. 2019;40:101506.
Yu S-J, Shen X F, Ge H-Q, Zheng H, Chu F-F, Hu H, et al. Role of sufficient phosphorous in biodiesel production from diatom Phaeodactylum tricornutum. Appl. Microbiol. Biotechnol. 2016; 100:6927-6934
Spijkerman E, Wacker A. Interactions between P-limitation and different C conditions on the fatty acid composition of an extremophile microalga. Extremophiles. 2011 1;15(5):597.
Esakkimuthu S, Krishnamurthy V, Govindarajan R, Swaminathan K. Augmentation and starvation of calcium, magnesium, phosphate on lipid production of Scenedesmus obliquus. Biomass Bioenergy 2016; 88: 126-134.
Goh B H H, Ong HC, Cheah MY, Chen W-H, Yu K L, Mahlia T M I. Sustainability of direct biodiesel synthesis from microalgae biomass: A critical review. Renew. Sustain. Energy Rev. 2019; 107: 59-74.
Finkle Yeh K-L, Chang J-S, Chen W-M. Effect of light supply and carbon source on cell growth and cellular composition of a newly isolated microalga Chlorella vulgaris ESP-31. Eng. Life Sci. 2010; 10: 201-208.
Gorain P C, Bagchi S K, Mallick N. Effects of calcium, magnesium and sodium chloride in enhancing lipid accumulation in two green microalgae. Environ.Technol. 2013; 34: 1887-1894.
Nelson DL, Cox MM. Lipid biosynthesis. In: Principles of biochemistry. 4th ed. New York: W. H. Freeman and Company. 2008; p. 805–845.
Lakshmikandan M, Murugesan AG, Wang S, Abomohra AE, Jovita PA, Kiruthiga S. Sustainable biomass production under CO2 conditions and effective wet microalgae lipid extraction for biodiesel production. J. Clean. Prod. 2020;247:119398.
Yeh K-L, Chang J-S, Chen W-M. Effect of light supply and carbon source on cell growth and cellular composition of a newly isolated microalga Chlorella vulgaris ESP-31. Eng. Life Sci. 2010; 10: 201-208.
Zhao G, Yu J, Jiang F, Zhang X, Tan T. The effect of different trophic modes on lipid accumulation of Scenedesmus quadricauda. Bioresour. Technol. 2012; 114: 466-471.
Li J, Li C, Lan C, Liao D. Effects of sodium bicarbonate on cell growth, lipid accumulation, and morphology of Chlorella vulgaris. Microb. Cell Fact. 2018; 17(1): 111.
Pradhan B, Baral S, Patra S, Behera C, Nayak R, MubarakAli D, et al. Delineation of gamma irradiation (60Co) induced oxidative stress by decrypting antioxidants and biochemical responses of microalga, Chlorella sp. Biocatal. Agric. Biotechnol. 2020; 1:101595.
Agarwal R, Rane S S, Sainis JK. Effects of (60) Co gamma radiation on thylakoid membrane functions in Anacystis nidulans. J. Photochem. Photobiol. B, Biol. 2008; 91: 9-19.
Fuma S, Ishii N, Takeda H, Miyamoto K, Yanagisawa K, Doi K, et al. Effects of acute gamma-irradiation on the aquatic microbial microcosm in comparison with chemicals. J. Environ. Rad. 2009; 100(12): 1027-1033.
Converti A, Casazza A A, Ortiz EY, Perego P, Del Borghi M . Effect of temperature and N concentration on the growth and lipid content of Nannochloropsis oculata and Chlorella vulgaris for biodiesel production. Chem. Eng. Process. 2009; 6:1146-1151.
Knothe G. Improving biodiesel fuel properties by modifying fatty ester composition. Energy Environ. Sci. 2009; 2: 759-766.
Knothe G. A technical evaluation of biodiesel from vegetable oils vs. algae. Will algae-derived biodiesel perform? Green Chem. 2011; 13: 3048-3065.
Ho SH, Chen WM, Chang JS. Scenedesmus obliquus CNW-N as a potential candidate for CO(2) mitigation and biodiesel production. Bioresour. Technol. 2010; 101: 8725-8730.
Knothe, G. Dependence of biodiesel fuel properties on the structure of fatty acid alkyl esters. Fuel Process. Technol. 2005; 86: 1059-1070.