Effect of Alstonia Boonei Stem Bark Extracts on the Activity of Liver Maker Enzymes in Rats Induced by Ccl4

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Victor E. Okpashi
Robert I. Uroko
Joy Ogana
A. Agbafor
C. P. Nwuke


This study investigated the outcome of Alstonia boonei stem bark on liver enzymes after inducing the Wistar albino rats with carbon tetrachloride (CCl4). This effect of plant extract was compared with silymarin – a drug commonly used for the treatment of chronic hepatocyte disorder. The plant sample was extracted with ethanol; acute toxicity study of the extract was performed on eighteen Wistar mice, while 30 rats were sacrificed for liver enzymes assay. The rats were divided into six clusters: each cluster has five rats, culster 1 served as control and was given 2 mL/kg b.w - distilled water; clusters 2 – 6 were CCl4 induced. Cluster 2 was untreated but served as the negative control while cluster 3 was given 0.025 mL or 25kg/kg B.W of Silymarin, which was a regular medicine and aided as the ordinary control. Rats in clusters 4 – 6 were administered - 100, 200 and 500 mg/kg, respectively of ethanol extract for fourteen days. The acute toxicity results of A. boonei extract showed relative fortification due to no death or adversarial responses after 24 hours of the extract administration. A substantial (P ≥ 0.05) surge in ALT action after administering 500 mg/kg proves lesser toxicity was greater dosage. At low dosage of the extract, a non-significant (p ≥ 0.05) rise in AST action specifies that the extract was moderately harmless with no hepatotoxic magnitude at low medication. The substantial reduction of alkaline phosphatase action in cluster 3 rats induced with CCl4 and given with Silymarin, together with clusters 4 – 6 that were CCl4 induced and administered with graded doses of A. boonei stem bark extract suggest hepatoprotective properties.


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Okpashi VE, Uroko RI, Ogana J, Agbafor A, Nwuke CP. Effect of Alstonia Boonei Stem Bark Extracts on the Activity of Liver Maker Enzymes in Rats Induced by Ccl4. Baghdad Sci.J [Internet]. 2022 Oct. 1 [cited 2022 Nov. 30];19(5):1058. Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/6107


Cragg G, Newman DJ. Biodiversity: a continuing source of novel drug leads. Pure Appl Chem. 2005;77(1):7–24. https://doi.org/10.1351/pac200577010007

Rufai SO, Olaniyi MB, Lawal IO. Evaluation of heavy metals in some selected medicinal plants growing within the University of Ibadan Campus. J med plant econ Dev. 2019: 3(1):a63. https://doi.org/10.4102/jomped.v3i1.63.

Derkach T, Khomenko V. Elemental Composition of the Medicinal Plants Hypericum perforatum, Urtica dioica and Matricaria chamomilla Grown in Ukraine: A Comparative Study. J Pharmacogn, 2018: 10(3):486–91. https://doi.org/10.5530/pj.2018.3.80

Akinmoladun AC, Ibukun EO, Afor E. Chemical constituents and antioxidant activity of Alstonia boonei. Afr J Biotechnol. 2007; 6(10); 1197–201.

Zongtai L, Dexi L, Lijie Z, Linghao L. Mineral Elements and Active Ingredients in Root of Wild Paeonia lactiflora Growing at Duolun County, Inner Mongolia. Biol Trace Elem Res. 2019; https://doi.org/10.1007/s12011-019-01725-3 PMID: 30997668.

Koduru S, Grierson DS, Afolayan AJ. Antimicrobial activity of Solanum aculeastrum (Solanaceae). Pharm Biol. 2006;44(4):284–6. https://doi.org/10.1080/13880200600714145.

Aida YT, Irina SD, Eduard VB, Anzhela AG. Bioaccumulation of Heavy Metals by Medicinal Plants of the Inula Genus. J Pharm Sci. Res. 2018;10(5):1263–6.

Igbinosa OO, Igbinosa EO, Aiyegoro OA. Antimicrobial activity and phytochemical gel: A review update. J Ethnopharmacol. 2009; 68, 3–37

Marian AN, Samuel TH, Lawrence SB, James HE. Heavy metal contents of some medicinal herbs from Kumasi, Ghana. Cogent Environ Sci. 2016;2:1234660. https://doi.org/10.1080/23311843.2016.1234660.

Farrugia A. Albumin usage in clinical medicine: tradition or therapeutic? Transfus Med Rev. 2010 Jan;24(1):53–63. https://doi.org/10.1016/j.tmrv.2009.09.005 PMID:19962575

Muhammad A, Muhammad A, Shaheen B, Azizullah A, Ruqia N, Shazia I. A review on the elemental contents of Pakistani medicinal plants: Implications forfolk medicines. J Ethnopharmacol, 2016; 188, 177–192.

Lorke D. A new approach to practical acute toxicity testing. Arch Toxicol. 1983 Dec;54(4):275–87. https://doi.org/10.1007/BF01234480 PMID:6667118

Glavač NK, Djogo S, Ražić S, Kreft S, Veber M. Accumulation of heavy metals from soil in medicinal plants. Arh Hig Rada Toksikol. 2017 Sep;68(3):236–44. https://doi.org/10.1515/aiht-2017-68-2990 PMID:28976884

Olateju DA. deyolanu OJK, Kayode SA, Gabriel AO, Manuel TMoral. Lead and cadmium contents in a medicinal plant/spice grown in an urban city of Nigeria. Cogent Food Agric. 2016; 2:1136016.

Suk KT, Kim MY, Baik SK. Alcoholic liver disease: treatment. World J Gastroenterol. 2014 Sep; 20(36):12934–44. https://doi.org/10.3748/wjg.v20.i36.12934 PMID:25278689

Uroko RI, Sangodare RS, Mohammad KH, Asadu CL. Effects of methanol extract of A. Pretorius leaves on male Wistar albino rats induced liver damage using carbon tetrachloride. J Biol Sci. 2015;15(3):116–23. https://doi.org/10.3923/jbs.2015.116.123

Goda N, Tachibana A, Okazawa G, Komatsu H. Representation of the material properties of objects in the visual cortex of nonhuman primates. J Neurosci. 2014 Feb;34(7):2660–73. https://doi.org/10.1523/JNEUROSCI.2593-13.2014 PMID:24523555

Samali A, Mohammed MI, Ibrahim MB. Analysis of Heavy Metals Concentration in Kano Herbal Preparations for Major Disease Conditions. Chem Search J. 2017;8(2):22–8.

Adie GU, Adekunle A. Evaluation of Potentially Toxic Metal Contamination of Local Medicinal Plants and Extracts Sold in Ibadan, Nigeria. J Health Pollut. 2017 Jun;7(14):23–9. https://doi.org/10.5696/2156-9614-7.14.23 PMID:30524819

Candy CÁ, Ángel JA, Juan JM, Alejandro HM. Quantitation of Cd, Pb and Fe in three medicinal plants (Justicia spicigera, Arnica montana and Hamelia pantens) from environmentally diverse locations of Huasteca Potosina. Mexico: Acta Univ; 2016. https://doi.org/10.15174/au.2016.984

Karahan F, Ozyigit II, Saracoglu IA, Yalcin IE, Ozyigit AH, Ilcim A. Heavy Metal Levels and Mineral Nutrient Status in Different Parts of Various Medicinal Plants Collected from Eastern Mediterranean Region of Turkey. Biol Trace Elem Res. 2019. https://doi.org/10.1007/s12011-019-01974-2 PMID: 31758293.

Uroko RI, Okpashi VE, Bayim PRB, Anthony UO, Ucho KM. Dietary effect of Alstonia Boonei Stem Bark extract on hematological profiles of Wistar albino rats after inducing oxidative stress with CCl4. Af J Bio.Sc. 2(4) (2020) doi:10.33472/AFJBS.2.4.2020.45-56.