The Association of Prothrombin Gene Mutations and Cytomegalovirus Infection with Abortion Among Iraqi Women

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Eman F Turkey
Abbas G Hamad
Abbas K AL-Mansoori


Abortion is categorized as the termination of conception caused by the failure or removal of the embryo from the uterus before the conclusion of pregnancy. Microorganisms and genetic factors are two of the many factors associated with abortion. Cytomegalovirus is a widespread congenital virus infection pathogen that affects a wide variety of people. The prothrombin gene is one of the essential causes that trigger blood clotting and the function of abortion women, therefore the aim of the study is to detect and associate Cytomegalovirus and prothrombin gene mutation (Gene ID: 14061 in NCBI) with abortion through genetic and immunological methods. Five ml of whole blood was collected from an intravenous puncture and divided into two tubes, one with EDTA and one without (plain tube) from 74 women with an abortion history as a patient group and 74 women without an abortion record who had at least one successful fertility as a control group. Allele-specific PCRs are used to amplify gene regions with genetic primers containing prothrombin gene polymorphisms. Current results have shown the greatest risk of abortion was observed in women patients with IgG seropositivity in 65 women with frequency (87.8%) and the lowest rate of abortion was in IgM seropositivity in 3 women with frequency (4.1%) and 6 (8.1%) were positive for IgM-and IgG indicating they have both an old and recent infections. Furthermore, allele-specific PCRs are used to amplify prothrombin G20201A polymorphism. The result of this study demonstrated there is no association between prothrombin genotype level frequency and abortion in CMV-infected women. While, there is a highly significant association between A and G Alleles combinations and abortion in CMV-infected women.


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Turkey EF, Hamad AG, AL-Mansoori AK. The Association of Prothrombin Gene Mutations and Cytomegalovirus Infection with Abortion Among Iraqi Women. Baghdad Sci.J [Internet]. 2022 Aug. 1 [cited 2023 Dec. 8];19(4):0768. Available from:


Mohamed A O, Hamza K M, Babker A M. Physiological changes in some haematological and coagulation profiles among Sudanese healthy pregnant women. Int J Med Sci Public Health. 2016; 5(3), 525-8. ‏

Elamin B K, Omer M O M A. Seroprevalence of cytomegalovirus infection among Sudanese women with recurrent pregnancy loss (RPL). Indian J Dent Res. 2015; 5(5), 4300-4302. ‏

Yalcintepe S, Ozdemir O, Hacivelioglu S O, Akurut C, Koc E, Uludag A, et al., Multiple inherited thrombophilic gene polymorphisms in spontaneous abortions in Turkish population. Int. J. Mol. Cell. Med. 2015; 4(2), 120. ‏

Homer H A. Modern management of recurrent miscarriage. Aust N Z J Obstet Gynaecol. 2019; 59(1), 36-44. ‏

Sultana S, Nallari P, Ananthapur V. Recurrent Pregnancy Loss (RPL): An overview. Int J Women's Health. 2020; 3(3), 302-315. ‏

Aboud R S. Detection of interleukin-6 and interleukin-8 in serum from women with recurrent spontaneous abortion. Baghdad Sci. J. 2010; 7(3), 1181- 1186. ‏

Hendriks E, MacNaughton H, MacKenzie M C. First trimester bleeding: evaluation and management. Am Fam Physician. 2019; 99(3), 166-174. ‏

Bilibio J P, Gama T B, Nascimento I C M, Meireles A J C, de Aguiar A S C, do Nascimento F C, et al. Causes of recurrent miscarriage after spontaneous pregnancy and after in vitro fertilization. Am J Reprod Immunol. 2020; 83(5), e13226. ‏

Leung K K, Hon K L, Yeung A, Leung A K, Man E. Congenital infections in Hong Kong: an overview of TORCH. Hong Kong Med J. 2020; 26(2), 127-138. ‏

Reeves M B. Cell signaling and cytomegalovirus reactivation: what do Src family kinases have to do with it? Biochem Soc Trans. 2020; 48(2), 667-675.

Layla A K Z, Ali K, Zgair M K, Ms F. Estimaion of LgM-anti HEV rubella and cytomegalovirus in the sera of aborted women. Baghdad Sci.J. 2006; 3(3), 445-448. ‏‏

Mohammed M K, Mohammed G S, Tawfeeq M S. Studying the ability of transformation of lymphocytes by using PHA in a patient with Cytomegalovirus. Baghdad Sci.J. 2010; 7(1), 334-340.

McCarty J M, Gierman E C, Bedell L, Lock M D, Bennett S. Safety and immunogenicity of live oral cholera vaccine CVD 103-hgr in children and adolescents aged 6–17 years. Am J Trop Med Hyg. 2020; 102(1), 48-57. ‏

Wang L, Xiang X, Mi B, Song H, Dong M, Zhang S, et al. Association between early prenatal exposure to ambient air pollution and birth defects: evidence from newborns in Xi’an, China. J Public Health (Oxf). 2019; 41(3), 494-501. ‏

Wawrusiewicz-Kurylonek N, Krętowski A J, Posmyk R. Frequency of thrombophilia associated genes variants: a population-based study. BMC Med Genet. 2020; 21(1), 1-5. ‏

Mohammed A I, Abdulqader A M R, Jalal S D, Mahmood S N. ABO Blood Groups and Thrombophilia Markers in Patients with Unstimulated Thrombosis in Kurdistan Region of Iraq. Clin. Appl. Thromb. Hemost. 2020; 26, 1-6. ‏

Kardi M T, Yousefian E, Allahveisi A, Alaee S. Association of factor V Leiden and prothrombin G20210A polymorphisms in women with recurrent pregnancy loss in Isfahan province, Iran. Int. J. Prev. Med. 2018; 9(13), 16-240. ‏

Gawish G, Al-Khamees, O. Molecular characterization of factor V Leiden G1691A and prothrombin G20210A mutations in Saudi females with recurrent pregnancy loss. Int J Blood Res Disord. 2013; 4, 165. ‏

EL Baz R A, Ramadan M M, Fayad E, Shaltot A A, Shershaby E M E. Comparative Study on the Effect of Factor V Leiden and Prothrombin Gene Polymorphism in Preeclampsia Cases. Egypt. J. Hosp. Med. 2014; 56(1), 345-354. ‏

Abdullah W Z, Kumaraguru S, Ghazali S, Yusoff N M. Factor V Leiden and prothrombin G20210A mutations among healthy Indians in Malaysia. Lab. Med. 2010; 41(5), 284-287. ‏

Sorlie D E. Medical biostatistics and epidemiology: Examination and Board review. 1st edition, McGraw-Hill Professional Publishing, the University of California, Appleton and Lange (1995); 47-88.

Vasani A, Kumar M S. Advances in the proteomics of amniotic fluid to detect biomarkers for chromosomal abnormalities and fetomaternal complications during pregnancy. Expert Rev Proteomic. 2019;16(4), 277-286. ‏

Yasir S J, AL-Khafaji Y A R, Al-Kelaby K K A. Cytomegalovirus UL83 Gene as Diagnostic Marker for Preliminary Infection in Pregnant Women in AL-Najaf City. Sys Rev Pharm. 2020; 11(5), 123-129. ‏

Anwar S A, Al-Bayati N S. Prevalence of Toxoplasma gondii and Cytomegalovirus in Sera of Aborted Women in Samarra city. Tikrit J. Pure sci. 2018; 22(6), 34-38. ‏

Dhami R M A, Kadhim B M, Abdullhusein H S. Serological Study to Diagnose the Causes of Recurrent Viral and Immune Miscarriage in Aborted Women Who Attend the Shatrah General Hospital. Int. J. Health Med. Sci. 2020; 3(1), 42-47. ‏

Saad F, Rahman Z U, Khan A, Noushin R, Ullah I, Dawar F U, et at. Detection of Cytomegalovirus in Pregnant Women of Lakki Marwat and Bannu, Khyber Pakhtunkhwa, Pakistan. Pak. J. Zool. 2021; 53(1), 363. ‏

Magnus M C, Wilcox A J, Morken N H, Weinberg C R, Håberg S E. Role of maternal age and pregnancy history in risk of miscarriage: prospective register-based study. Brit. Med. J. 2019; 364: l869, 1-8. ‏

Aslan I, Ozyigit S, Paul L T, Tosun O, Tulay P. Thrombophilia associated gene polymorphisms: Does use of medication, including anti-coagulants, minerals or folic acid, prevent the miscarriages? Am J Reprod Immunol. 2020; 141, 1-4. ‏

Rafiei S T, Mohammadian T, Rasi H. Common Polymorphisms in MTHFR and Prothrombin Gene in Iranian Women with Abortions at Different Ages. Obstet. gynecol. int. j. 2017; 8(2), 00286. ‏

Gawish G E. Molecular characterization of factor V Leiden G1691A and prothrombin G20210A mutations in Saudi newborns with stroke. Biochem. Genet. 2011; 49(9), 601-610. ‏