Toxicity of Nanomulsion of Castor Oil on the Fourth larval stage of Culex quinquefsciatus under Laboratory Conditions

Main Article Content

Soolaf A Kathiar
https://orcid.org/0000-0002-6720-8741
Alla Abd Al Hassan Al Hilfy
Hazim Idan Al Shammari

Abstract

Mosquitoes like Culex quinquefasciatus are the primary vector that transmits many causes of diseases such as filariasis, Japanese encephalitis, and West Nile virus, in many countries around the world. The development in the scientific fields, such as nanotechnology, leads to use this technique in control programs of insects including mosquitoes through the use of green synthesis of nanoemulsions based on plant products such as castor oil.  Castor oil nanoemulsion was formulated in various ratios comprising of castor oil, ethanol, tween 80, and deionized water by ultrasonication. Thermodynamic assay improved that the formula of (10 ml) of castor oil, ethanol (5ml), tween 80 (14 ml) and deionized water (71ml)   was more stable than other formulas. The formulated castor oil nanoemulsion was characterized by transmitting electron microscopy and dynamic light scattering.  Nanoemulsion droplets were spherical in shape and found to have a Z-average diameter of 93 nm. A concentration of castor oil nanoemulsion (250, 350, 450, and 550 ppm) was tested as larvicidal agents and bulk emulsion (1000, 1500, 2000, and 2500 ppm) was tested also and compared against the fourth instar larvae. Our nanoemulsion showed higher activity when compared to bulk emulsion.   LC50 for castor oil nanoemulsion and castor bulk emulsion were found to be 268.21 and 409.37ppm after 72 h, respectively. The biochemical assays were carried out to examine the effect of castor oil nanoemulsion on biochemical characteristics of larvae. The treated larval homogenate showed inhibition in the activity of acetylcholinesterase.

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Toxicity of Nanomulsion of Castor Oil on the Fourth larval stage of Culex quinquefsciatus under Laboratory Conditions. Baghdad Sci.J [Internet]. 2022 Oct. 1 [cited 2024 Mar. 29];19(5):0999. Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/6638
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How to Cite

1.
Toxicity of Nanomulsion of Castor Oil on the Fourth larval stage of Culex quinquefsciatus under Laboratory Conditions. Baghdad Sci.J [Internet]. 2022 Oct. 1 [cited 2024 Mar. 29];19(5):0999. Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/6638

References

Rueda L M. Global diversity of mosquitoes (Insecta: Diptera: Culicidae) in freshwater. Hydrobiologia .2007; 595: 477- 487.

Dahmana H, Mediannikov O. Mosquito-Borne Diseases Emergence/Resurgence and How to Effectively Control It Biologically. Pathogens .2020; 9, 310.

Ibanez-Justicia A, Smits N, Hartog W, Vossenberg B, De Wolf K, Deblauwe I, Van Bortel W, Jacobs F, et al. Detection of Exotic Mosquito Species (Diptera: Culicidae) at International Airports in Europe. Int. Environ. Res. Public Health. 2020 May; 17, 3450.

Oliver J, Tan Y, Haight Jamie D, Tober Keith J, Gall Wayne K , Zink Steven D ,et al . Spatial and temporal expansions of Eastern equine encephalitis virus and phylogenetic groups isolated from mosquitoes and mammalian cases in New York State from 2013 to 2019. Emerg. Microbes Infect. 2020; 9: 1638-1650.

Guo X, Li Chun – xiao, Deng Yong – qiang, Xing Dan, Liu Qin – mei, Wu Qun, et al. Culex pipiens quinquefasciatus: a potential vector to transmit Zika virus. Emerg. Microbes Infect. 2016 Sep; 5; e120.

Benelli G. Review: Managing mosquitoes and ticks in a rapidly changing world – Facts and trends. Saudi J Biol Scie. 2018 Jun; 26: 921 – 929.

Mossa Abdel – Tawab. Green Pesticides: Essential Oils as Biopesticides in Insect-pest Management. J Environ. Sci Tech. 2016 Aug; 9: 354 – 378.

Yaseen A T, Sulaiman K A. Insecticidal Activity of Some Chemicals of Mosquitoes Culex pipiens molestus Forskal. Baghdad Sci. J. 2021 Mar; 18 (1): 716- 721.

Perumalsamy H, Jin Jang M, Kim J R, Kadarkaria M, Ahn Y J. Larvicidal activity and possible mode of action of four flavonoids and two fatty acids identified in Millettia pinnata seed toward three mosquito species. Parasit Vectors. 2015 Apr; 8: 237.

Kumuda S S, Mohan K T, Vijayan V A. Larvicidal, Ovicidal, Oviposition Deterrent Efficacy of Extraction of Two Plant Species Against Culex quinquefasciatus say, at Mysuru , India . Inter J Curr Res. 2019 Feb; 11 (02): 1484 – 1488.

Yaseen Aulfat T. The Effect of Alcoholic and Aqueous Extract of Piper nigrum on the Larvae of Culex pipiens molestus Forskal (Diptera: Culicid). Baghdad J Sci .2020 mar; 17(1): 28-33.

Yeboah A, Ying Sh, Lu Jiannong, Xie Yu, Amoanimaa – Dede H, Boateng K G, et al . Castor oil (Ricinus communis): a review on the chemical composition and physicochemical properties. Food Sci Tech. 2020 Jul. DOI: https: // doi . org / 10 – 1590 / fst. 19620.

Kodjo T A, Gbenonchi M, Sadate A, Komi A, Yaovi G, Dieudonne M, et al. Bio-insecticidal effects of plant extracts and oil emulsions of Ricinus communis L. (Malpighiales: Euphorbiaceae) on the diamondback, Plutella xylostella L. (Lepidoptera: Plutellidae) under laboratory and semi-field conditions. J Appl Bio Sci 2011 Jan; 43: 2899 – 2914.

Ubaid J. Fumigant Toxicity of Ricinus communis L. Oil on Adults and Larva of Some Stored Product Insects. J Natural Sci. Res. 2014; 4 (4).

Sogan N, Kapoor N, Singh H, Kala S, Navak A., Nagbal B.N. Larvicidal activity of Ricinus communis extract against mosquitoes. J. Vector Borne Dis. 2018 Dec; 55: 282 – 290.

Mohamed Abdel – Raheem. Nano Essential Oils against cotton leaf worm, Spodoptera littoralis (Boisduval) (Lepidoptera: Noctuid). J Chem Tech Res. 2019; 12 (05): 123 – 128.

Gupta A, Eral H.B, Hatton TA, Doylep.S. Nanoemulsions: formation, properties and applications. Soft. Matter. 2016; 12: 1-17.

Jesus Flavia L M, Almeida Fernanda B, Ouarte J, Oliveira Anna, Crus R, Souto R, et al. Preparation of a Nanoemulsion with Carapa guianensis Aublet (Meliaceae) Oil by a Low-Energy/Solvent-Free Method and Evaluation of Its Preliminary Residual Larvicidal Activity. Evid.-based Complement Altern Med. 2017 Jul; Doi: https: // doi.org /10.1155/2017/6756793.

Jesser E, Lorenzetti A S, Yeguerman C, Murray A P , Domini C , Wlerdin – Gonzales J . Ultrasound assisted formation of essential oil nanoemulsions: Emerging alternative for Culex pipiens pipiens Say (Diptera: Culicidae) and Plodia interpunctella Hübner (Lepidoptera: Pyralidae) management. Ultrason Sonochem. 2020 Mar; 61:104832. doi: 10.1016/j.ultsonch.2019.104832. Epub 2019 Oct 18. PMID: 31675660.

Pranita S, Amrita B. Nanoemulsions- a Review. Int j res pharm chem. .2016; 6(2): 312 – 322.

Ghnimi W. PORIM Test Methods. Palm Oil Research Institute of Malaysia Test Methods. Ministry of Primary Industries, Malaysia, 1995; pp. 83-121.

WHO. Guidelines for laboratory and field testing of mosquito larvicides. (CDS/WHOPES/GCDPP/05.13), 2005. World Health Organization. https://apps.who.int/iris/handle/10665/69101

Ismail S Mansour. Effect of sublethal doses of some insecticides and their role on detoxication enzymes and protein-content of Spodoptera littoralis (Boisd.) (Lepidoptera: Noctuidae) . Bull Natl Res Cent .2020; 44 (35).

Abbott, W.S. A method of computing the effectiveness of an insecticide. J. Econ. Entomol . 1925; 18: 265-267.

Bali V, Ali M, Ali J. Study of surfactant combinations and development of a novel nanoemulsion for minimising variations in bioavailability of ezetimibe. Colloids Surf. B Biointerfaces .2010; 76, 410–420. doi: 10.1016/j.colsurfb.2009.11.021.

Sogan N, Kapoor N, Kala S, Patanjali P K, Nagpal B N, Vikram K, et al. Larvicidal activity of castor oil Nanoemulsion against malaria vector Anopheles culicifacies. Inter. J. Mosq Res. 2018 Apr; 5 (3): 01 – 06.

Sugumar S, Clarke S K, Nirmala M J, Tryagi B K, Mukherjee A, Chandrasekaran N. Nanoemulsion of eucalyptus oil and its larvicidal activity against Culex quinquefasciatus . "Bull. Entomol. Res. 2014 Jan ;104: 393- 402.

Ramos-López MA, Pérez GS, Rodríguez-Hernández C, Guevara-Fefer P, Zavala-Sánchez MS. Activity of Ricinus communis (Euphorbiaceae) against Spodoptera frugiperda (Lepidoptera: Noctuidae). African J. Biotech. 2010; 9(9):1359-1365.

Attaullah Z, Zahoor M K, Mubarik M S, Rizvi H, Majeed H N, Zulhussnian M, et al. Insecicidal , Biological and Biochemical Response of Musca domestica ( Diptera :Muscidae ) to some Indigenous Weed Plant Extracts . Saudi J. Bio Sci. 2020; 27 (1), 106 – 116.

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