Effect of Secondary Metabolite Crude of Metarhizum anisopliea Fungus on the Second Larval Stage of the Housefly Musca domestica

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Soolaf A Kathiar
Hazim Idan Al Shammari
Aliaa Abdul Aziz Hammed


         The house flies Musca domestica )Diptera:musidae) are the primary carrier of many pathogens such as cholera, typhoid, anthrax, and others. The use of chemical pesticides as a basic method of control leads to many problems at the environmental and health level. The use of safe alternatives to chemical pesticides has become an urgent necessity. The research aims to find biological alternatives that are environment-friendly and non-pathogenic to humans in controlling house flies through the possibility of extracting and diagnosing some secondary metabolites produced by the fungus Metarhizium anisopliae and testing their effects on the second larval stage of house flies using different treatment methods that include direct spraying of the larvae, treating the food environment, and the dipping method. Secondary metabolites and toxins of Metarhizium anisopliae were extracted in liquid media PDB using a mixture of organic solvents such as ethyl acetate and methanol. The secondary metabolites were identified by gas chromatography-mass spectrometry (GC-MS). The results showed the identification of 10 chemical compounds, including phenol, 2,4-bis(1,1-dimethylethyl (C14H22O)., Diethyl Phthalate (C12H14O4),  Hexadecanoic acid, methyl ester (C17H34O2 ),  Phthalic acid, butyl undecyl ester (C23H36O4) , 9,12-Octadecanoic (Z,Z )-, methyl ester ( C19H34O2),  9-Octadecanoic acid, methyl ester, (C19H36O2), 9,12,15-Octadecanoic acid,methyl ester,(Z,Z,Z) (C19H32O2), Octadecanoic acid,methyl ester(C19H38O2), Oleic Acid (C24H38O4), 9-Octadecanoic acid (Z)-,2-hydroxyl (hydroxymethyl) ethyl ester(C21H40O4) ,and Di-n-octyl phthalate (C24H38O4 ). The results showed that the crude extract of the fungus cause the best mortality rate  in the second instar larvae at concentrations of 3 and 5% after 72 hours of treatment when  the mortality rates ranged between 60-100%. The mortality rates were directly proportional to the increase in concentration and time with a significant difference. The results also showed that the treatment of the food media was the most effective in affecting the larvae of flies, recording mortality rates that reached 100%, with a significant difference with direct spraying and dipping methods. These results reveal the significant efficacy of the tested secondary metabolite crude of m.anesopalae against Musca domestica which could be used as an ecofriendly alternative for insect control.


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Kathiar SA, Al Shammari HI, Hammed AAA. Effect of Secondary Metabolite Crude of Metarhizum anisopliea Fungus on the Second Larval Stage of the Housefly Musca domestica. Baghdad Sci.J [Internet]. 2022 Dec. 5 [cited 2023 Jan. 28];19(6(Suppl.):1493. Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/7035


Adenusi AA and Adewoga TO. Prevalence of helminthic ova Human intestinal parasites in non-biting in Shiraz playground and recovering of E. coli synanthropic flies in Ogum State, Nigeria. 2013a. Med Infect Dis. 11: 181–9.

Onyenwe E, Okore OO, Ubiaru PC, Abel C. Housefly-borne helminth parasites of Mouau and its public health implication for the university community. Anim Res Int. 2016; 13(1): 2352–2358.

Geden C J, Nayduch D, Scott J G, Burgess IV E R, Gerry A C, Kaufman P E, et al. House Fly (Diptera: Muscidae): Biology, Pest Status, Current Management Prospects, and Research Needs. J Integr Pest Manag. 2021; 12(1): 39; 1–38

Köhl J, Kolnaar R, Ravensberg WJ. Mode of action of microbial biological control agents against plant diseases: Relevance beyond efficacy. J Front Plant Sci 2019. Jul 19. 10: 845. doi: 10.3389/fpls.2019.00845.

Yaseen AT, Sulaiman K A. Insecticidal Activity of Some Chemicals of Mosquitoes Culex pipiens molestus Forskal. Baghdad Sci J. 2021 Mar; 18 (1): 716- 721.

Yaseen Aulfat T. The Effect of Alcoholic and Aqueous Extract of Piper nigrum on the Larvae of Culex pipiens molestus Forskal (Diptera: Culicid). Baghdad J Sci .2020 mar; 17(1): 28-33.

Rosa E, Ekowati C N, Handayani T T, Widiastuti E L. Isolation and identification entomopathogen fungi as candidate of bioinsecticide from flies and cockroaches’ (Insect

vector’s disease). J Phys: Conf Ser. 2021; 1751: 012049.

Bamisile BS, Akutse KS, Siddiqui JA, Xu Y. Model Application of Entomopathogenic Fungi as Alternatives to Chemical Pesticides: Prospects, Challenges, and Insights for Next-Generation Sustainable Agriculture. Front Plant Sci. 2021; 12: 7 41804. doi: 10.3389/fpls.

Abdullah RRH. The side effect of commonly used chemical pesticides on entomopathogenic Beauveria bassiana and Bacillus thuringiensis as biopesticides. Egypt J Plant Prot Res Inst. 2019. 2019a; 2(1): 1 8.

Ahmad W (eds), Khan Md A. Microbes for Sustainable lnsect Pest Management, Sustainability in Plant and Crop Protection. 2021; 17, Springer Nature Switzerland AG.

Avalos J, Limón M C. Fungal Secondary Metabolism. Encyclopedia. 2022: 2:1–13. https://doi.org/10.3390/ encyclopedia 2010001

Garcia C J. Mycotoxins study: Toxicology, Identification and Control. Toxins. 2021; 13: 242.

Farooq, M, Shoaib F. Infectivity of housefly, Musca domestica (Diptera: Muscidae) to different entomopathogenic fungi. Environmental Microbiology. Braz J Microbiol. 2016 .47 (4): 807–816

Hamamaa H M, Zyaanb O H, Abu Alic O A, Saleh D I, Elakkad H A, El-Saadonye M T, et al. Virulence of entomopathogenic fungi against Culex pipiens: Impact on biomolecules availability and life table parameters . Saudi J Biol Sci. 2022; 29: 385-393.

Hermize F B, Ahmed R F, Abed-Ali H M. Biological and physiological effects of Coriandrum sativum on House fly Musca domestica (Diptera: Muscidae). Baghdad J Sci. 2016, (1):34, 14-19.

Junaid Z, Rana F Sh, Yuxin Z, Shoaib F, Xiaoxia X, Fengliang J. Metarhizium anisopliea Challenges Immunity and Demography of Plutella xylostella. Insects. 2020 Oct; 11(10): 694.

Quesada-Moraga E. Destruxin A production by Metarhizium brunneum strains during transient endophytic colonisation of Solanum tuberosum. Biocontrol Sci Technol. 2016; 26: 1574–1585.

Hoe P K, Bong C F J, Jugah K, Rajan A. Evaluation of Metarhizium anisopliae var. anisopliae (Deuteromycotina: Hyphomycete) isolates and their effects on subterranean termite Coptotermes curvignathus (Isoptera: Rhinotermitidae). Am J Agric Biol Sci. 2009; 4: 289-297.

Ortiz – Urquiza A, Riveiro-Miranda C. Santiago-A.Ivarez, and E. Quesada-Moraga.. Insect –toxic secreted proteins and irulence of the entomopathogenic fungus Beauveria bassiana . J Invertebr Pathol. 2010; 105: 270-278.

Vivekanandhan P, Swathy K, Kalaimurugan D, Ramachandran M, Yuvaraj A, Kumar AN, et al. Larvicidal toxicity of Metarhizium anisopliae metabolites against three mosquito species and nontargeting organisms. PLoS ONE. 2020; 15(5). JiangShiou Hwang, National Taiwan Ocean University, Taiwan.

Vivekanandhan P, Thangaraj K, Sengodan K, Sengottayan S N, Muthugoundar S S. Toxicity of Beauveria bassiana28 Mycelial Extracts on Larvae of Culex quinquefasciatus Mosquito (Diptera: Culicidae). Int J Environ Res Public Health 2018; 15(3): 440; https://doi.org/10.3390/ijerph15030440 .

Xia J, Psychogios N, Young N, Wishart D S. Metabolic Analysis server for metabolomic data analysis and interpretation. Nucleic Acids Res. 2009; 37: 12

Bali V, Ali M, Ali J. Study of surfactant combinations and development of a novel nanoemulsion for minimising variations in bioavailability of ezetimibe. Colloids Surf B Biointerfaces. 2010; 76, 410–420.

Skrobe P, Krishan K M, Padma K. Gas Chromatography -Mass Spectrometric analysis of acetone extract of Marwar dhaman grass for bioactive compounds. Plant Arch. 2015; 15(2): 1065-1074.

Fuqiang Z, Ping W, Rima D L, Zushang Su, Shiyou Li. Natural Sources and Bioactivities of 2,4-Di-Tert-Butylphenol and Its Analogs. Toxins. 2020, 12(1): 35.

Yong-Jiang Xu , Feifei Luo, Bing Li, Yanfang Shang, and Chengshu Wang. Metabolic Conservation and Diversification of Metarhizium Species Correlate with Fungal Host-pecificity. j Front Microbiol. 2016; 7 :12

Abdullah R R H. Insecticidal Activity of Secondary Metabolites of Locally Isolated Fungal Strains against some Cotton Insect Pests. J plant prot Pathol. 2019; 10 (12): 647-653.

Tulika T, Mala A. Phytochemical screening and GC-MS analysis of bioactive constituents in the ethanolic extract of Pistia stratiotes L. and Eichhornia crassipes (Mart.) solms. J Pharmacogn Phytochem 2018; 6 (1): 195-206.

Skrobek, A, Tariq M Butt. Toxicity testing of destruxins and crude extracts from the insect – pathogenic fungus Metarhizium anisopliae. school of biological sciences, university of Wales Swansea, singleton Park, Swansea SA2 8PP, UK. 2005

Farooq M, Shoaib F. Infectivity of housefly, Musca domestica (Diptera:Muscidae) to different entomopathogenic fungi . Braz J Microbiol 2016; 47: 807-816.

Litwin A, Monika N, Sylwia R. Entomopathogenic fungi: unconventional applications. Rev Environ Sci Biotechnol. 2020 19: 23–42.

Molnar I, Gibson D M, Krasnoff S B. Secondary metabolites from Entomopathogenic Hypocrealean fungi. Nat Prod Rep. 2010; 27 (9): 1241–1275.

Keyhani, N. O. Lipid biology in fungal stress and virulence: Entomopathogenic fungi. Fungal Biol. 2018, 122(6): 420–429.

Liang S, Liang K. Millet grain as a candidate antioxidant food resource: a review. Int J Food 2019; 22(1): 1652-1661