In vitro isolation and expansion of neural stem cells NSCs

Authors

  • Maeda H. Mohammad Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetics Research, Al-Mustansiriyah University, Baghdad, Iraq. https://orcid.org/0000-0002-0877-2780
  • Aous Kahtan Almzaien Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetics Research, Al-Mustansiriyah University, Baghdad, Iraq. https://orcid.org/0000-0002-3283-9754
  • Ahmad A. Al-Joubory Neuroscience Hospital, Baghdad, Iraq.
  • Ahmed M. Al-Shammari Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetics Research, Al-Mustansiriyah University, Baghdad, Iraq. https://orcid.org/0000-0002-2699-1514
  • Ayser A. Ahmed Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetics Research, Al-Mustansiriyah University, Baghdad, Iraq.
  • Hiba K. Shaker Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetics Research, Al-Mustansiriyah University, Baghdad, Iraq.
  • Aseel K. Abedalsattar Experimental Therapy Department, Iraqi Center for Cancer and Medical Genetics Research, Al-Mustansiriyah University, Baghdad, Iraq.

DOI:

https://doi.org/10.21123/bsj.2022.7280

Keywords:

Cryopreservation, Isolation, Nestin, Neural stem cells, Neurosphere, Stem cells

Abstract

   Neural stem cells (NSCs) are progenitor cells which have the ability to self‑renewal and potential for differentiating into neurons, oligodendrocytes, and astrocytes. The in vitro isolation, culturing, identification, cryopreservation were investigated to produce neural stem cells in culture as successful sources for further studies before using it for clinical trials. In this study, mouse bone marrow was the source of neural stem cells. The results of morphological study and immunocytochemistry of isolated cells showed that NSCs can be produced successfully and maintaining their self‑renewal and successfully forming neurosphere for multiple passages. The spheres preserved their morphology in culture and cryopreserved to be a ready source for use in experiments as a model for neurological disorders.

Received 2/4/2022

Accepted 28/6/2022

Published Online First 20/11/2022

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Published

2023-06-01

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Vol. 20 No. 3 (2023): Issue 3

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article

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1.
In vitro isolation and expansion of neural stem cells NSCs. Baghdad Sci.J [Internet]. 2023 Jun. 1 [cited 2024 Apr. 27];20(3):0787. Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/7280
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