An Evaluation of the Activity of Prepared Zinc Nanoparticles with Extracted Alfalfa Plant in the Treatment of Heavy Metals

Main Article Content

Zainab Ridha Al-Bahadili
Abbas Ali Salih AL-Hamdani
Farqad Abdullah Rashid
Labeeb Ahmed Al-Zubaidi
Suha Mohamed Ibrahim


The alfalfa plant, after harvesting, was washed, dried, and grinded to get fine powder used in water treatment. We used the alfalfa plant with ethanol to make the alcoholic extract characterized by using (GC-Mass, FTIR, and UV) spectroscopy to determine active compounds. Alcoholic extract was used to prepare zinc nanoparticles. We characterized Zinc nanoparticles using (FTIR, UV, SEM, EDX Zeta potential, XRD, AFM). Zinc nanoparticle with Alfalfa extract and alfalfa powder were used in the treatment of water polluted with inorganic elements such as Cr, Mn, Fe, Cu, Cd, Ag by (Batch processing). The batch process with using alfalfa powder gets treated with Pb (51.45%), which is the highest percentage of treatment. Mn (13.18%), which is the lowest percentage of treatment. The batch process with using Zinc nanoparticles gets the result treated with Pb(98.822%), which is the highest percentage of treatment, and Mn (10.31%), which is the lowest percentage of treatment. When comparing alfalfa powder and zinc nanoparticle, it has been found that the treatment with zinc nanoparticle is more efficient in the removal of inorganic pollutants.


Download data is not yet available.

Article Details

How to Cite
Al-Bahadili ZR, AL-Hamdani AAS, Rashid FA, Al-Zubaidi LA, Ibrahim SM. An Evaluation of the Activity of Prepared Zinc Nanoparticles with Extracted Alfalfa Plant in the Treatment of Heavy Metals. Baghdad Sci.J [Internet]. 2022 Dec. 5 [cited 2023 Dec. 8];19(6(Suppl.):1399. Available from:


Ajmi RN, Sultan M, Hanno SH. Bioabsorbent of Chromium, Cadmium and Lead from Industrial waste water by Waste plant. J Pharm Sci Res. 2018; 10(3): 672-674.

Al-Hadithy AH, Motlag KH, Sharaf ME, Hashim LQ. Using of Rustumiya sewage water for irrigation: 1-its effect on some soil properties and corn growth. Baghdad Sc J. 2011; 8(1): 179-187.‎

Shakir HI, Al-Hamdani AAS, Rashid FA, Al-Zubaidi LA. Estimation of some selected organic and inorganic pollutants in industrial wastewater and tigris river. Turkish J Phys Reh. 2021; 32(3): 16710-16715.

Al Zoubi W, Jirjees V, Suleman V, Al-Hamdani AAS, Ahmed SD, Kim YG, et al. Synthesis and bioactivity studies of novel Schiff bases and their complexes. J Phys Org Chem. 2019; 32(11): e4004.

Xia J, Duan QY, Luo Y, Xie ZH, Liu ZY, Mo XG. Climate change and water resources: Case study of Eastern Monsoon Region of China. Adv Clim Change Res. 2017 Jun 1; 8(2): 63-7.

Risan RS, Al-Hamdani AAS, Hassan ZF. Determination of Metal ions, Phenols, and NO3-, NO2- in Industrial Wastewater of Al-Dura and Al-Najaf Refinery. Int J Psych Rehab. 2020; 24(5): 5132-5141.

Sadiq H, Sher F, Sehar S, Lima EC, Zhang S, Iqbal HM, et al. Green synthesis of ZnO nanoparticles from SyzygiumCumini leaves extract with robust photocatalysis applications. J Mol Liq. 2021Aug 1; 335: 116567.

Jayanthi P, Lalitha P. Reducing power of the solvent extracts of Eichhornia crassipes (Mart.) Sold. Int. J Phar Phar Sci. 2011; 3(3): 126-8.

Matinise N, Fuku XG, Kaviyarasu K, Mayedwa N, Maaza MJ. ZnO nanoparticles via Moringa oleifera green synthesis: Physical properties & mechanism of formation. App Sur Sci. 2017 Jun 1; 406: 339-47.

Christian P, Kammer FV, Baalousha M, Hofmann H. Nanoparticles: Structure, Properties, Preparation and Behaviour in Environmental Media. Ecotoxicology. 2008; 17(5): 326-43.

Wannas NM, Al-Hamdani AAS, Al-Zoubi W. Spectroscopic characterization for new complexeswith 2,2'- (5,5-dimethylcyclohexane-1,3-diylidene) bis (azan-1-yl-1-ylidene) dibenzoic acid. J Phys Org Chem. 2020Jul; 33(11): 1-12.

Jirjees VY, Al‐Hamdani AAS, Wannas NM, Farqad AR, Adnan D, Al-Zoubi W. Spectroscopic characterization for new model from Schiff base and its complexes. J Phys Org Chem. 2021 April, 34(4): e4169.

Kareem MJ, Al-Hamdani AAS, Young GK, Al-Zoubi W, Saad GM. Synthesis, characterization, anddetermination antioxidant activities for new Schiffbase complexes derived from 2-(1H-indol-3-yl)-ethylamine and metal ion complexes. J Mol Struct. 2021 May; 1231: 129669.

Al-Zoubi W, Kim MJ, Yoon DK, Al-Hamdani AA, Kim YG, Young GK. Effect of organic compounds and rough inorganic layer formed by plasma electrolytic oxidation on photocatalytic performance. J Alloys Compd. 2020 May 15; 823: 153787.

Al-Hamdani AAS, Hamoodah RG. Transition metal complexes with tridentate ligand: preparation, spectroscopic characterization, thermal analysis and structural studies. Baghdad Sci. J. 2016; 13(4): 770-781.

Kareem MJ, Al-Hamdani AAS, Vian YJ, Mohammad EK, Abdul WA, Al Zoubi W. Preparation, spectroscopic study of Schiff base derived fromdopamine and metal Ni(II), Pd(II), and Pt(IV)complexes, and activity determination as antioxidants. J Phys Org Chem. 2021 Mar; 34 (3): e4156.

Al-Hamdani AAS. Synthesis, Characterization, Theoretical, thermal Studies and Bio Activities of Metal Complexes with Schiff base mannich ligand. Res J Pharm Biol Chem Sci. 2017 May 1; 8(3): 2119-2132.

Fadhel AM, Al-Hamdani AAS. Synthesis, spectroscopic, characterization and biological studies for new complexes with N1, N5-Bis (3 hydroxyphenyl)- 2-oxopentanediamide. Turkish J Phys Reh 2021; 32(3): 11864-11876.

Hadioui M, Merdzan V, Wilkinson KJ. Detection and characterization of ZnO nanoparticles in surface and waste waters using single particle ICPMS. Sci Tech. 2015; 49(10): 6141–6148.

Hartmann G, Schuster M. Species selective preconcentration and quantification of gold nanoparticles using cloud point extraction and electrothermal atomic absorption spectrometry. Anal Chim Acta 2013; 761: 27-33.

Lingmei L, Yang W, Li Q, Gao S, Shang JK. Synthesis of Cu2O nanospheres decorated with TiO2nanoislands, their enhanced photoactivity stability under visible light illumination, and theirpost-illumination catalytic memory. ACS Appl Mater. Interfaces 2014; 6, 5629–5639.

Daneshvar N, Aber S, Dorraji S, Khataee MS, Rasolifard AR. Preparation and investigation of photocatalytic properties of ZnO nanocrystals: effect of operational parameters and kinetic study. Int J Chem Bio Eng. 2008; 1(1): 24-29.

Al Zoubi W, Al-Hamdani AAS, Sunghun B, Ko YG. A review on TiO2-based composites for superior photocatalytic activity. Rev Inorg Chem. 2021; 41(4): 213-222.

Al-Bahadili ZR, Al-Hamdani AAS, Al-Zubaidi LA, Rashid FA, Ibrahim SM. An Evaluation of the Activity of Prepared Zinc Nano-Particles with Extract Alfalfa Plant in the Treatments of Peptidase and Ions in Water. Chem Methodol. 2022; 6(7): 522-533.

Aljabali AAA, Yazan AY, Al Zoubi MS, Al-Batayneh KhM, Al-Trad KhMB, Alrob OA, et al. Synthesis of Gold Nanoparticles Using Leaf Extract of Ziziphus zizyphus and their Antimicrobial Activity. Nanomaterials. 2018 Mar; 8(3): 174.

Ma H, Williams PL, Diamond SA. Ecotoxicity of manufactured ZnO nanoparticles-A review. Environ Pollut. 2013; 172: 76-85.

Rahman A, De Clippeleir H, Thomas W, Jimenez JA, Wett B, Al-Omari A, et al. A-stage and high-rate contact-stabilization performance comparison for carbon and nutrient redirection from high-strength municipal wastewater. Chemic Eng J. 2019 Feb 1; 357: 737-49.

Irvine RL, Busch AW. Sequencing batch biological reactor: an overview. J Water Poll Con Fed. 1979; 51: 235.