Effect of Green-biosynthesis Aluminum Nanoparticles (Al NPs) on Salmonella enterica Isolated from Baghdad City
DOI:
https://doi.org/10.21123/bsj.2023.7526Keywords:
Al NPs, Biosynthesized Al NPs, Antimicrobial effect of Al NPs, Foodborne disease, Salmonellosis.Abstract
This study is aimed to Green-synthesize and characterize Al NPs from Clove (Syzygium aromaticum
L.) buds plant extract and to investigate their effect on isolated and characterized Salmonella enterica growth.
S. aromaticum buds aqueous extract was prepared from local market clove, then mixed with Aluminum nitrate
Al(NO3)3. 9 H2O, 99.9% in ¼ ratio for green-synthesizing of Al NPs. Color change was a primary confirmation
of Al NPs biosynthesis. The biosynthesized nanoparticles were identified and characterized by AFM, SEM,
EDX and UV–Visible spectrophotometer. AFM data recorded 122nm particles size and the surface roughness
RMs) of the pure S. aromaticum buds aqueous extract recorded 17.5nm particles size, while the results of Al
NPs in the tested sample recorded 21nm particles size with surface roughness RMs about 2.35nm. SEM images
revealed the presence of Al NPs with diameters ranged from 33.5-70.4nm with regular spiracles shape particles
in the prepared biosynthesized nanoparticle sample. The EDX spectrum analysis showed that the Aluminium
weight ratio was 1.75, while it was 50.498 in the Al NPs sample prepared from aqueous extract. UV-Visible
spectroscopy data revealed that biosynthesized Al NPs were absorbed at 213nm while Aluminum nitrate was
absorbed at 258nm. These results indicate the formation of Al NPs. The antibacterial activity showed that Al
NPs exhibited having high antibacterial activity on Salmonella spp. isolates compared to the effect of the
control agent (imipenem) in this experiment. We conclude that biosynthesized Al NPs from clove aqueous
extract can be exploited as natural antibacterial compounds to inhibit the growth of Salmonella.
Received 9/6/2022
Revised 3/10/2022
Accepted 4/10/2022
Published Online First 20/3/2023
References
Gnach A, Lipinski T, Bednarkiewicz A, Rybka J, Capobianco JA. Up converting nanoparticles: assessing the toxicity. Chem Soc Rev. 2015; 44(6): 1561–1584. https://doi.org/10.1039/c4cs00177j.
Bayda S, Adeel M, Tuccinardi T, Cordani M, Rizzolio F. The History of Nanoscience and Nanotechnology: From Chemical–Physical Applications to Nanomedicine. Molecules. 2019; 25(1): 112. https://doi.org/10.3390/molecules25010112.
Kaur B, Markan M, Singh M. Green Synthesis of Gold Nanoparticles from Syzygium aromaticum Extract and Its Use in Enhancing the Response of a Colorimetric Urea Biosensor. Bionanoscience. 2012; 2(4): 251–258. https://doi.org/10.1007/s12668-012-0062-5.
Sharma VK, Yngard RA, Lin Y. Silver nanoparticles: Green synthesis and their antimicrobial activities. Adv Colloid Interface Sci. 2009; 145: 83–96. https://doi.org/10.1016/j.cis.2008.09.002.
Huang J, Li Q, Sun D, Lu Y, Su Y, Yang X, et al. Biosynthesis of silver and gold nanoparticles by novel sundried Cinnamomum camphor leaf. Nanotechnology. 2007; 18(10):105104. https://doi.org/10.1088/0957-4484/18/10/105104.
Sreelakshmy V, Deepa MK, Mridula P. Green synthesis of silver nanoparticles from Glycyrrhiza glabra root extract for the treatment of gastric ulcer. J Develop Drugs. 2016; 5(152): 2(1-5). https://doi.org/10.4172/2329-6631.1000152.
Ju-Nam Y, Lead JR. Manufactured nanoparticles: An overview of their chemistry, interactions and potential environmental implications. Sci. Total Environ. 2008; 400: 396–414. https://doi.org/10.1016/j.scitotenv.2008.06.042.
Ealia SA, Saravanakumar MP. A review on the classification, characterisation, synthesis of nanoparticles and their application. In IOP Conference Series: Mater Sci Eng A. IOP Publishing. 2017; 263(3): 032019. https://doi.org/10.1088/1757-899x/263/3/032019.
Boles MA, Engel M, Talapin DV. Self-assembly of colloidal nanocrystals: From intricate structures to functional materials. Chem Rev. 2016;116(18): 11220-89. https://doi.org/10.1021/acs.chemrev.6b00196.
Sasidharan S, Raj S, Sonawane S, Sonawane S, Pinjari D, Pandit AB, et al. Nanomaterial synthesis: chemical and biological route and applications in Nanomaterials Synthesis. Elsevier sci. 2019: 27-51. https://doi.org/10.1016/b978-0-12-815751-0.00002-x.
Ijaz I, Gilani E, Nazir A, Bukhari A. Detail review on chemical, physical and green synthesis, classification, characterizations and applications of nanoparticles. Green Chem Lett Rev. 2020; 13(3): 223–245. https://doi.org/10.1080/17518253.2020.1802517.
Usman AI, Aziz AA, Noqta OA. Green nonchemical synthesis of gold nanoparticles using palm oil leaves extracts. Mater Today: Proc. 2019; 7: 803–807. https://doi.org/10.1016/j.matpr.2018.12.078.
Rauwel P, Kuunal S, Ferdov S, Rauwel E. A Review on the Green Synthesis of Silver Nanoparticles and Their Morphologies Studied via TEM. Adv Mater Sci Eng. 2015; 2015: 1–9. https://doi.org/10.1155/2015/682749.
Diego CR, Wanderley OP. Clove (Syzygium aromaticum): a precious spice. Asian Pac. J Trop Biomed. 2014; 4(2): 90–96. https://doi.org/10.1016/s2221-1691(14)60215-x.
El-Saber Batiha G, Alkazmi LM, Wasef LG, Beshbishy AM, Nadwa EH, Rashwan EK. Syzygium aromaticum L. (Myrtaceae): Traditional Uses, Bioactive Chemical Constituents, Pharmacological and Toxicological Activities. Biomolecules. 2020; 10(2): 202. https://doi.org/10.3390/biom10020202.
Mittal M, Gupta N, Parashar P, Mehra V, Khatri M. Phytochemical evaluation and pharmacological activity of Syzygium aromaticum: a comprehensive review. Int J Pharm Sci. 2014; 6(8): 67-72.
Sharma N, Sharma P. Review Article On Synthesis and Applications of Aluminium. Int Res J Eng Technol. 2020; 7: 730-733.
Doskocz N, Affek K, Zalęska-Radziwill M. Effects of aluminium oxide nanoparticles on bacterial growth. E3S Web Conf. 2017; 17: 00019. https://doi.org/10.1051/e3sconf/20171700019.
Salem SS, Fouda A. Green Synthesis of Metallic Nanoparticles and Their Prospective Biotechnological Applications: An Overview. Biol Trace Elem Res. 2020; 199(1): 344–370. https://doi.org/10.1007/s12011-020-02138-3.
Bintsis T. Foodborne pathogens. AIMS microbiology. 2017; 3(3): 529–563. https://doi.org/10.3934/microbiol.2017.3.529.
Wei B, Shang K, Cha SY, Zhang, JF, Jang HK, Kang M. Clonal dissemination of Salmonella enterica serovar albany with concurrent resistance to ampicillin, chloramphenicol, streptomycin, sulfisoxazole, tetracycline, and nalidixic acid in broiler chicken in Korea. Poult. Sci J. 2021; 100(7): 101141. https://doi.org/10.1016/j.psj.2021.101141.
Fadilah F, Widodo VA, Priawan RP, Joyo EO, Abdurrohman H, Paramita RI, et al. Synthesis Nanoparticle extract of Clove (Syzygium aromaticum L.) and Characterization by Differential Scanning Colorimetry Profiling and its Activities as Inhibitor of HeLa Cell lines. Res J Pharm Technol. 2019; 12(7): 3355. https://doi.org/10.5958/0974-360x.2019.00566.3.
Hasanpoor M, Fakhr Nabavi H, Aliofkhazraei M. Microwave-assisted synthesis of alumina nanoparticles using some plants extracts. J Nanostruct. 2017; 7(1): 40-46.
Dikshit P, Kumar J, Das A, Sadhu S, Sharma S, Singh S, et al. Green Synthesis of Metallic Nanoparticles: Applications and Limitations. J Catal. 2021; 11(8): 902. https://doi.org/10.3390/catal11080902.
Chicea D. Nanoparticles and nanoparticle aggregates sizing by DLS and AFM. Optoelectron. Adv. Mater. 2010; 4(9): 1310-5.
Selvi KV, Sivakumar T. Isolation and characterization of silver nanoparticles from Fusarium oxysporum. Int J Curr Microbial Appl. sci. 2012; 1(1): 56-62.
Hudzicki J. Kirby-Bauer disk diffusion susceptibility test protocol. ASM. 2009; 15: 55-63.
CLSI. Performance standards for antimicrobial susceptibility testing. 31th (ed.). CLSI document M100. Wayne, USA. 2021; p 7.
Hernandez‐Diaz JA, Garza‐Garcia JJ, Zamudio‐Ojeda A, Leon‐Morales JM, Lopez‐Velazquez JC, Garcia‐Morales S. Plant‐mediated synthesis of nanoparticles and their antimicrobial activity against phytopathogens. J Sci Food Agric. 2021; 101(4): 1270-1287. https://doi.org/10.1002/jsfa.10767.
El-Shafey AM. Green synthesis of metal and metal oxide nanoparticles from plant leaf extracts and their applications: A review. Green Process Synth. 2020; 9(1): 304–339. https://doi.org/10.1515/gps-2020-0031.
Salih TA, Hassan KT, Majeed SR, Ibraheem IJ, Hassan OM, Obaid AS. In vitro scolicidal activity of synthesized silver nanoparticles from aqueous plant extract against Echinococcus granulosus. Biotechnol Rep. 2020; 28: e00545(1-5). https://doi.org/10.1016/j.btre.2020.e00545.
Dhar SA, Chowdhury RA, Das S, Nahian MK, Islam D, Gafur MA. Plant-mediated green synthesis and characterization of silver nanoparticles using Phyllanthus emblica fruit extract. Mater Today: Proc. 2021; 42: 1867–1871. https://doi.org/10.1016/j.matpr.2020.12.222.
Adewale OB, Egbeyemi KA, Onwuelu JO, Potts-Johnson SS, Anadozie SO, Fadaka AO, et al. Biological synthesis of gold and silver nanoparticles using leaf extracts of Crassocephalum rubens and their comparative in vitro antioxidant activities. Heliyon. 2020; 6(11): e05501(1-10). https://doi.org/10.1016/j.heliyon.2020.e05501.
Hammodi H, Rashid I, Oraibi A. Green Biosynthesis, Identification and Characterization of Ag and Zn Nanoparticles Using Ivy (Epipremnum Aureum) Plant Extract. Plant Arch. 2019; 19(2): 959-965.
Ghotekar S. Plant extract mediated biosynthesis of Al₂O₃ nanoparticles-a review on plant parts involved, characterization and applications. Nanochem Res. 2019; 4(2): 163-169. https://doi.org/10.22036/ncr.2019.02.008.
Asano N, Lu J, Asahina S, Takami S. Direct Observation Techniques Using Scanning Electron Microscope for Hydrothermally Synthesized Nanocrystals and Nanoclusters. Nanomaterials (Basel). 2021; 11(4): 908. https://doi.org/10.3390/nano11040908.
Sagadevan S, Koteeswari P. Analysis of structure, surface morphology, optical and electrical properties of copper nanoparticles. J Nanomed Res. 2015; 2(5): 00040-00048. https://doi.org/10.15406/jnmr.2015.02.00040.
Scimeca M, Bischetti S, Lamsira HK, Bonfiglio R, Bonanno E. Energy Dispersive X-ray (EDX) microanalysis: A powerful tool in biomedical research and diagnosis. Eur J Histochem. 2018; 62(1): 2841. https://doi.org/10.4081/ejh.2018.2841.
Iqbal T, Ejaz A, Abrar M, Afsheen S, Batool SS, Fahad M, et al. Qualitative and quantitative analysis of nanoparticles using laser-induced breakdown spectroscopy (LIBS) and energy dispersive x-ray spectroscopy (EDS). Laser Phys. 2019; 29(11): 116001. https://doi.org/10.1088/1555-6611/ab3fa1.
Prashanth PA, Raveendra RS, Hari Krishna R, Ananda S, Bhagya NP, Nagabhushana BM, et al. Synthesis, characterizations, antibacterial and photoluminescencestudies of solution combustion-derived Al₂O₃ nanoparticles, J Asian Ceram Soc. 2015; 18(3): 345-351. https://doi.org/10.1016/j.jascer.2015.07.00.
Agudelo W, Montoya Y, Bustamante J. Using a non-reducing sugar in the green synthesis of gold and silver nanoparticles by the chemical reduction method. DYNA. 2018; 85(206): 69–78. https://doi.org/10.15446/dyna.v85n206.72136.
Panariello L, Radhakrishnan AN, Papakonstantinou I, Parkin IP, Gavriilidis A. Particle Size Evolution during the Synthesis of Gold Nanoparticles Using in Situ Time-Resolved UV–Vis Spectroscopy: An Experimental and Theoretical Study Unravelling the Effect of Adsorbed Gold Precursor Species. J Phys Chem C. 2020; 124(50): 27662–27672. https://doi.org/10.1021/acs.jpcc.0c07405.
Al-nassar SI, KM A, Mahdi ZF. Study the effect of different liquid media on the synthesis of alumina (Al₂O₃) nanoparticle by pulsed laser ablation technique. CIRP J Manuf Sci Technol. 2015; 3(4): 77-81. https://doi.org/10.13189/mst.2015.030401.
Anand U, Carpena M, Kowalska-Goralska M, Garcia-Perez P, Sunita K, Bontempi E, et al. Safer plant-based nanoparticles for combating antibiotic resistance in bacteria: A comprehensive review on its potential applications, recent advances, and future perspective. Sci Total Enviro. 2022; 821: 153472. https://doi.org/10.1016/j.scitotenv.2022.153472.
Bansal H, Kaushal J, Chahar V, Shukla G, Bhatnagar A. Green Synthesis of Silver Nanoparticles Using Syzygium aromaticum (Clove) Bud Extract: Reaction Optimization, Characterization and Antimicrobial Activity. J Bionanosci. 2018; 12(3): 378–389. https://doi.org/10.1166/jbns.2018.1531.
Kavitha R, Valli C, Karunakaran R, Vijayarani K, Amutha R. Evaluation of Antibacterial Activity of some Indian Herbal Extracts. Int J Curr Microbiol. 2020; 9(7): 17–24. https://doi.org/10.20546/ijcmas.2020.907.003.
Afanyibo YG. Antimicrobial Activities of Syzygium aromaticum (L.) Merr. and LM Perry (Myrtaceae) Fruit Extracts on Six Standard Microorganisms and Their Clinical Counterpart. Open Access Libr. 2018; 5(12): 1-12. https://doi.org/10.4236/oalib.1104951.
Abd El-Aziz DM, Yousef NM. Enhancement of antimicrobial effect of some spices extract by using biosynthesized silver nanoparticles. Int Food Res J. 2018; 25(2), 589-596.
Ali D, Ismail M, Hashem MA, Akl MA. Antibacterial Activity of Eco Friendly Biologically Synthesized Copper Oxide Nanoparticles. Egypt J Chem. 2021; 64(8): 4099-4104. https://doi.org/10.21608/EJCHEM.2021.67430.3481.
Matsakova EG, Simakova DI. Nanoparticles Manifesting Antibacterial Effects: Properties, Production, Mechanism of Action, and Applications. Nanotechnologies Russ. 2020; 15(2): 236–240. https://doi.org/10.1134/s1995078020020159.
Radifand HM, Al-Bahrani RM. Green synthesized of silver nanoparticles and study their properties and applications. J Biotech Res. 2019; 13(2): 5–9. https://doi.org/10.24126/jobrc.2019.13.2.575.
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