تأثير جزيئات الألمنيوم النانوية المصنعة الخضراء Al NPsعلى السالمونيلا المعوية المعزولة من مدينة بغداد
DOI:
https://doi.org/10.21123/bsj.2023.7526الكلمات المفتاحية:
جزيئات الألومينا النانوية، Al NPs المركب حيوياً، التأثير المضاد للميكروبات لـ Al NPs، الأمراض المنقولة بالغذاء، السالمونيلا.الملخص
هدفت هذه الدراسة إلى التوليف الأخضر وتوصيف جسيمات الألومينا النانوية Al NPs من مستخلص نبات القرنفل (Syzygium aromaticum L.) وللتحقق من تأثيرها على نمو السالمونيلا المعوية المعزولة والمشخصة. تم تحضير المستخلص المائي لبراعم القرنفل من نبات القرنفل المحلي، ثم خلط مع نترات الألومنيوم Al(NO₃)₃. 9 H₂O، 99.9٪ المحضر بنسبة ¼ لتخليق أخضر لـ Al NPs. تغير اللون كان تأكيدًا أوليًا للتخليق الحيوي لـ Al NPs. تم التعرف على الجسيمات النانوية المركبة حيويا وتشخيصها عن طريق AFMو SEMو EDX ومقياس الطيف الضوئي المرئي للأشعة فوق البنفسجية. أظهرت بيانات AFM حجم جسيمات 122 نانومتر وخشونة السطح RMs للمستخلص المائي لبراعم S. aromaticum النقية التي تسجل حجم جسيمات 121 نانومتر، بينما سجلت نتائج Al NPs في العينة المختبرة حجم جسيمات 21 نانومتر مع خشونة السطح RMs) حوالي 21 نانومتر. وقد حددت صور SEM وجودAl NPs بأقطار تتراوح من 33.5-70.4 نانومتر مع جزيئات منتظمة الشكل في عينة الجسيمات النانوية المحضرة حيوياً. أظهر تحليل طيف EDX أن نسبة وزن الألمنيوم كانت 1.75، وكانت 50.498 في عينة Al NPs المُصنَّعة حيوياً المحضرة من المستخلص المائي لبراعم S. aromaticum. كشفت بيانات التحليل الطيفي المرئي للأشعة فوق البنفسجية أن Al NPs المركب حيوياً قد تم امتصاصه عند 213 نانومتر بينما تم امتصاص نترات الألومنيوم عند 258 نانومتر. تشير هذه النتائج إلى تكوين Al NPs. أظهر النشاط المضاد للبكتيريا أن Al NPs أظهر نشاطًا عاليًا مضادًا للبكتيريا على Salmonella spp. مقارنة بتأثير عامل التحكم (imipenem) في هذه التجربة. نستنتج، بأنه يمكن استخدام Al NPs المركب حيويًا من مستخلص القرنفل المائي كمركبات طبيعية مضادة للجراثيم لمنع نمو بكتيريا Salmonella.
Received 9/6/2022
Revised 3/10/2022
Accepted 4/10/2022
Published Online First 20/3/2023
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