سمية الكلوربيريفوس على ذوات الصدفتين في المياه العذبة (Unio tigridis) وتأثيراتها على المؤشرات الحيوية
محتوى المقالة الرئيسي
الملخص
للذوات الصدفیتین وظيفة هامة في المياه العذبة إذ تقوم بعملية تصفية المياه ولكون مكوثهم في البيئة القعرية يؤثرون على زيادة نسبة الأوكسجين مما يؤدي الى بيئة قاعیه اكثر حيوية. الهدف من الدراسة هو معرفة كيفية تأثير الكلوربيريفوس على الخصائص الكيميائية الحيوية في ذات الصدفيتيين في المياه العذبة Unio tigridis. تم جمع حوالي 180 كائن إضافة إلى عينة من موارود مياه قنديل على نهر الزاب الكبير في محافظة اربيل – العراق. بمجرد وصولهم إلى المختبر ، تم الاحتفاظ بالأفراد في أحواض مع مياه النهر ودرجة حرارة الغرفة المكيفة: 25 ± 2 والضوء: 12 ساعة / 12 ساعة والتأقلم مع ظروف المختبر لمدة سبعة أيام في ماء منزوع الكلور. يتضمن تحديد بلح البحر جزيئيًا وتسلسل الحمض النووي لبلح البحر رقم انضمام بنك الجينات المقدم من U. tigridis ON872361 و ON872362 و ON872363 و ON872364. تم فحص سمية مبيد كلوربيريفوس لمدة ستة و تسعون ساعة وتأثيرها على U. Tigridis من خلال استخدام التراكيز المختلفة تتراوح ما بين 50, 100, 200, 300 and 400 جزء في المليون. تم تقييم الصفات الفيزيائية والكيميائية لمياه النهر والاحواض بما في ذلك درجة حرارة وإ الأس الهيدروجيني ، والتوصيل الكهربائي ، وإجمالي المواد الصلبة الذائبة ، والأكسجين المذاب ، واالقاعدية، و العسرة ، وأيون الكالسيوم ، وأيون المغنيسيوم. و أظهرت نتائج فحص مياه الأحواض بأنها مناسبة لتربية الكائنات، ثم تم ملاحظة الكائنات الميتة وكانت قيمة التركيز المميت النصفي بعد 96 ساعة لذات الصدفيتين 157.99 جزء في المليون. وضمن خياشيم الكائن المدروس تم فحص AChE, GST, CAT, و MDA . و كانت النتيجة بأن تعرض للكلوربيریفوس یۆدی إلی الزيادة في GTS, CAT, MDA ولكن ارتبط ارتفاع مؤشر حيوية للإجهاد التأكسدي عكسياً مع AChE فی الكائن المدروس. و تم استنتاج بأن تلوث المياه بالكلوربيريفوس يؤدي إلى ظروف غير آمنة للكائنات المائية.
Received 14/10/2022
Revised 18/12/2022
Accepted 20/12/2022
Published Online First 20/5/2023
تفاصيل المقالة
هذا العمل مرخص بموجب Creative Commons Attribution 4.0 International License.
كيفية الاقتباس
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