Treatment with Dielectric Barrier Discharge (DBD) plasma restricts Aspergillus niger growth isolated from wheat grain

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Thikra K. Al-Khafaji


Microbiological contamination by fungi impacts the quality and safety of wheat grain storage. This study aimed to evaluate the efficacy of cold plasma in restricting the growth of the fungus, Aspergillus niger, which was isolated from wheat grains. A dielectric barrier discharge (DBD) operating at atmospheric pressure generated cold plasma that was used to treat the fungus, and the impact of this treatment was investigated at various periods  1, 2, 4, 6, and 15 minutes. The results revealed a highly significant decrease in the growth and number of spores of Aspergillus niger compared to the controls. This study revealed an efficient technique for enhancing wheat grain storage that could be a foundation for further large-scale studies.


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Al-Khafaji TK. Treatment with Dielectric Barrier Discharge (DBD) plasma restricts Aspergillus niger growth isolated from wheat grain. Baghdad Sci.J [Internet]. 2023 Aug. 30 [cited 2023 Oct. 4];20(4(SI):1480-8. Available from:
Special Issue - Current advances in anti-infective strategies


Molina-Hernandez JB, Laika J, Peralta-Ruiz Y, Palivala VK, Tappi S, Cappelli F, et al. Influence of Atmospheric Cold Plasma Exposure on Naturally Present Fungal Spores and Physicochemical Characteristics of Sundried Tomatoes (Solanum lycopersicum L.). Foods. 2022; 11(2): 210.

Harlan JR, Zohary D. Distribution of wild wheats and barley: the present distribution of wild forms may provide clues to the regions of early cereal domestication. Science. 1966; 153(3740): 1074-80.

Los A, Ziuzina D, Boehm D, Bourke P. Effects of cold plasma on wheat grain microbiome and antimicrobial efficacy against challenge pathogens and their resistance. Int J Food Microbiol. 2020; 335: 108889.

Ling L, Jiafeng J, Jiangang L, Minchong S, Xin H, Hanliang S, et al. Effects of cold plasma treatment on seed germination and seedling growth of soybean. Sci Rep. 2014; 4(1): 1-7.

Ortiz R, Sayre KD, Govaerts B, Gupta R, Subbarao G, Ban T, et al. Climate change: can wheat beat the heat? Agric Ecosyst Environ. 2008; 126(1-2): 46-58.

Guo Q, Meng Y, Qu G, Wang T, Yang F, Liang D, et al. Improvement of wheat seed vitality by dielectric barrier discharge plasma treatment. Bioelectromagnetics. 2018; 39(2): 120-31.

Chauhan NM, Washe AP, Minota T. Fungal infection and aflatoxin contamination in maize collected from Gedeo zone, Ethiopia. Springer Plus. 2016; 5(1): 1-8.

Awuchi CG, Ondari EN, Eseoghene IJ, Twinomuhwezi H, Amagwula IO, Morya S. Fungal growth and mycotoxins production: Types, toxicities, control strategies, and detoxification. Fungal reproduction and growth. 2021; 100207.

Vasiliki H, Nicholas S. Potentially toxigenic fungi from selected grains and grain products. J Food Saf. 2017; 38(1).

Chandravarnan P, Agyei D, Ali A. Green and sustainable technologies for the decontamination of fungi and mycotoxins in rice: A review. Trends Food Sci Technol. 2022.

Gao Y, Soubani A. Advances in the diagnosis and management of pulmonary aspergillosis. Adv Respir Med. 2019; 87(6): 231-43.

Vojkovská H, Slámová J, Kozáková Z, KRČMA F. Study of sterilization effect of dielectric barrier discharge on eucaryotic microorganisms. Publications of the Astronomical Observatory of Belgrade. 2010: 331-4.

Lee Y, Lee YY, Kim YS, Balaraju K, Mok YS, Yoo SJ, et al. Enhancement of seed germination and microbial disinfection on ginseng by cold plasma treatment. J Ginseng Res. 2021; 45(4): 519-26.

Stolárik T, Henselová M, Martinka M, Novák O, Zahoranová A, Černák M. Effect of low-temperature plasma on the structure of seeds, growth and metabolism of endogenous phytohormones in pea (Pisum sativum L.). Plasma Chem. Plasma Process. 2015; 35(4): 659-76. DOI

Sohbatzadeh F, Mirzanejhad S, Shokri H, Nikpour M. Inactivation of Aspergillus flavus spores in a sealed package by cold plasma streamers. J Theor Appl Phys. 2016; 10(2): 99-106.

Ji SH, Kim T, Panngom K, Hong YJ, Pengkit A, Park DH, et al. Assessment of the effects of nitrogen plasma and plasma‐generated nitric oxide on early development of Coriandum sativum. Plasma Process Polym. 2015; 12(10): 1164-73.

Holubová Ľ, Kyzek S, Ďurovcová I, Fabová J, Horváthová E, Ševčovičová A, et al. Non-thermal plasma—a new green priming agent for plants? Int J Mol Sci. 2020; 21(24): 9466.

Priatama RA, Pervitasari AN, Park S, Park SJ, Lee YK. Current Advancements in the Molecular Mechanism of Plasma Treatment for Seed Germination and Plant Growth. Int J Mol Sci. 2022; 23(9): 4609.

Hameed T, Kadhem S. Plasma diagnostic of gliding arc discharge at atmospheric pressure. Iraqi J Sci . 2019: 2649-55. DOI: 10.24996/ijs.2019.60.12.14

Hameedl T, Kadhem S, editors. Gliding arc discharge for water treatment.

IOP Conf Ser Mater Sci Eng; 2020: IOP Publishing.

Alothman ZA, Bahkali AH, Khiyami MA, Alfadul SM, Wabaidur SM, Alam M, et al. Low cost biosorbents from fungi for heavy metals removal from wastewater. Sep Sci Technol. 2020; 55(10): 1766-75.

Pitt JI, Hocking AD. Fungi and food spoilage: Springer; 2009.

Burmeister L. The antagonistic mechanisms employed by Trichoderma harzianum and their impact on the control of the bean rust fungus Uromyces appendiculatus: Hannover: Gottfried Wilhelm Leibniz Universität Hannover; 2008.

Al-Ameri HA. Defining Mycotoxins Associated with Wheat Grains in Mosul Silo by ELISA. J Hunan Univ Nat Sci. 2022; 49(4): 345-352.

Senanayake I, Rathnayaka A, Marasinghe D, Calabon M, Gentekaki E, Lee H, et al. Morphological approaches in studying fungi: Collection, examination, isolation, sporulation and preservation. Mycosphere. 2020; 11(1): 2678-754.

FEI L-w, LU W-b, XU X-z, YAN F-c, ZHANG L-w, LIU J-t, et al. A rapid approach for isolating a single fungal spore from rice blast diseased leaves. J Integr Agric. 2019; 18(6): 1415-8.

Green MR, Sambrook J. Estimation of cell number by hemocytometry counting. Cold Spring Harb Protoc. 2019; 2019(11): pdb. prot097980.

Murillo D, Huergo C, Gallego B, Rodríguez R, Tornín J. Exploring the Use of Cold Atmospheric Plasma to Overcome Drug Resistance in Cancer. Biomedicines. 2023; 11(1): 208.

Chen Z, Chen G, Obenchain R, Zhang R, Bai F, Fang T, et al. Cold atmospheric plasma delivery for biomedical applications. Mater Today (Kidlington). 2022.

Fricke K. Influence of non-thermal plasma-based biological decontamination processes on the surface properties of plasma-exposed. Polymers. 2012.

Mostofian B, Zhuang T, Cheng X, Nickels JD. Branched-chain fatty acid content modulates structure, fluidity, and phase in model microbial cell membranes. J Phys Chem B. 2019; 123(27): 5814-21.

Waldie S, Sebastiani F, Browning K, Maric S, Lind TK, Yepuri N, et al. Lipoprotein ability to exchange and remove lipids from model membranes as a function of fatty acid saturation and presence of cholesterol. Biochim Biophys Acta Mol Cell Biol Lipids. 2020; 1865(10): 158769.

Sakudo A, Misawa T. Antibiotic-resistant and non-resistant bacteria display similar susceptibility to dielectric barrier discharge plasma. Int J Mol Sci. 2020; 21(17): 6326.

Polčic P, Machala Z. Effects of non-thermal plasma on yeast saccharomyces cerevisiae. Int J Mol Sci. 2021; 22(5): 2247.

Crestale L, Laurita R, Liguori A, Stancampiano A, Talmon M, Bisag A, et al. Cold atmospheric pressure plasma treatment modulates human monocytes/macrophages responsiveness. Plasma. 2018; 1(2): 261-76.

Obileke K, Onyeaka H, Miri T, Nwabor OF, Hart A, Al‐Sharify ZT, et al. Recent advances in radio frequency, pulsed light, and cold plasma technologies for food safety. J Food Process Eng. 2022; 45(108): e14138.