Single Nucleotide Polymorphism (SNP) rs2229569 with L Selectin Gene Expression in Iraqi Female during in vitro Fertilization Program.
DOI:
https://doi.org/10.21123/bsj.2024.8832Keywords:
Gene expression, Gene polymorphism rs2229569, Infertility, In vitro Fertilization, Selectin L.Abstract
Infertility is recognized as one of the leading reproductive health problems in various regions of the world. In vitro fertilization (IVF) is one of the most effective treatments for infertility. In the current research, the role of the selectin -L (SELL) gene, especially the rs2229569 polymorphism, has been determined in terms of implantation and expression level. This work involved 67 females who underwent an in vitro fertilization cycle, divided into two major groups: the implantation failure female group and the implantation success female group. Blood samples were collected from the females. After DNA extraction from blood sample then amplification by polymerase chain reaction, samples were sent for sequence analyses. The SNP rs2229569 was detected and that recorded three genotypes. The failure group had a deviation from H.W.E., and the success group was in agreement with H.W.E. law according to Chi square values (X2 = 11.60, 1.58). Significant differences in the failure group refers to the effects of infertility in the study population. Odds ratio of rs2229569 showed that the T allele was (2.09), so T allele may be considered a risk allele for the failure implantation. Finally, the SELL gene expression had a downregulation in female with failed implantation compared to female in successful implantation groups.
Received 31/03/2023
Revised 14/08/2023
Accepted 16/08/2023
Published Online First 20/02/2024
References
Namdar A, Naghizadeh MM, Zamani M, Yaghmaei F, Sameni MH. Quality of life and general health of infertile women. healthcare. 2017; 15(1): 1-7. https://doi.org/10.1186/s12955-017-0712-y .
Sirait BI, Reviani N, Udjung GI. Factors Affecting Infertility in Women of Reproductive Age in the IVF Programme. Int. J. Trop. Dis. Health. 2023; 44(1): 65-75. https://doi.org/10.9734/ijtdh%2F2023%2Fv44i11386.
Greil AL, Slauson‐Blevins K, McQuillan J. The experience of infertility: a review of recent literature Sociol Health Illn. 2010; 32(1): 140-62. https://doi.org/10.1111/j.1467-9566.2009.01213.x .
Zaami S, Driul L, Sansone M, Scatena E, Andersson KL, Marinelli E. ART innovations: fostering women’s psychophysical health between bioethics precepts and human rights. In Healthcare. 2021; 9 (11) :1486. https://doi.org/10.3390/healthcare9111486 .
Su RW, Fazleabas AT. Regulation of Implantation and Establishment of Pregnancy in Mammals. 6th ed. USA: Springer Cham, 2015. Chapter 10, Implantation and establishment of pregnancy in human and nonhuman primates; p.189-213. https://doi.org/10.1007/978-3-319-15856-3_10 .
6.Massimiani M, Lacconi V, La Civita F, Ticconi C, Rago R, Campagnolo L. Molecular signaling regulating endometrium–blastocyst crosstalk. Int J Mol Sci. 2019; 21(1): 23. https://doi.org/10.3390/ijms21010023 .
Darvishi B, Boroumandieh S, Majidzadeh-A K, Salehi M, Jafari F, Farahmand L. The role of activated leukocyte cell adhesion molecule (ALCAM) in cancer progression, invasion, metastasis and recurrence: A novel cancer stem cell marker and tumor-specific prognosticmarker. Exp.J.Mol.Pathol.2020; 115:104443. https://doi.org/10.1016/j.yexmp.2020.104443 .
Homrich M, Gotthard I, Wobst H, Diestel S. Cell adhesion molecules and ubiquitination—functions and significance. Biology. 2015; 5(1): 1. https://doi.org/10.3390/biology5010001 .
AL-Maini EH, Ali SI. The correlation between serum levels of soluble platelet-selectin in preeclampsia with & without intrauterine growth retardation versus normal pregnancy. J .Fac Med Baghdad. 2017; 59(1): 36-41. https://doi.org/10.32007/jfacmedbagdad.591156 .
Sehring J, Beltsos A, Jeelani R. Human implantation: The complex interplay between endometrial receptivity, inflammation, and the microbiome. Placenta. 2022; 117: 179-86. https://doi.org/10.1016/j.placenta.2021.12.015 .
.Ashary N, Tiwari A, Modi D. Embryo implantation: war in times of love. Gen. Comp. J Endocrinol. 2018; 159(2): 1188-98. https://doi.org/10.1210/en.2017-03082 .
Dai W, Ma L, Cao Y, Wu D, Yu T, Zhai J. In vitro fertilization outcome in women with endometrial tuberculosis and tubal tuberculosis. Gynecol Endocrinol. 2020; 36(9): 819-23. https://doi.org/10.1080/09513590.2019.1702639 .
.Ochoa-Bernal MA, Fazleabas AT. Physiologic events of embryo implantation and decidualization in human and non-human primates. Int J Mol Sci. 2020; 21(6): 1973. https://doi.org/10.3390/ijms21061973 .
Zhong M, Huang J, Wu Z, Chan KG, Wang L, Li J, et al. Potential Roles of Selectins in Periodontal Diseases and Associated Systemic Diseases: Could They Be Targets for Immunotherapy? Int J Mol. 2022; 23(22): 14280. https://doi.org/10.3390/ijms232214280 .
National Center for Biotechnology Information(https://www.ncbi.nlm.nih.gov/).
.Almohaidi AMS, Salman IN, Kaduhm RM. Study ABO/Rh system with Endothelial Inflammatory Factors in Iraqi Arab Female with Diabetes Mellitus Type II. Baghdad Sci J. 2014; 11(1): 93-102. https://doi.org/10.21123/bsj.2014.11.1.93-102 .
.Lai TH, Chang FW, Lin JJ, Ling QD. Gene expression of human endometrial L-selectin ligand in relation to the phases of the natural menstrual cycle. Sci Rep. 2018; 8(1): 1443. https://doi.org/10.1038/s41598-018-19911-z .
Foulk RA, Zdravkovic T, Genbacev O, Prakobphol A. Expression of L-selectin ligand MECA-79 as a predictive marker of human uterine receptivity. J Assist Reprod Genet. 2007 Jul; 24: 316-21. https://doi.org/10.1007/s10815-007-9151-8 .
.Jozefczuk J, Adjaye J. Quantitative real-time PCR-based analysis of gene expression. Methods Enzymol. 2011; 500: 99-109. Academic Press. https://doi.org/10.1016/B978-0-12-385118-5.00006-2 .
Wei YS, Lan Y, Meng LQ, Nong LG. The association of L-selectin polymorphisms with L-selectin serum levels and risk of ischemic stroke. J Thromb. 2011; 32: 110-5. https://doi.org/10.1007/s11239-011-0587-4 .
Al KA, Almohaidi AMS. Polymorphism variation of l-selectin the pro213ser (rs2229569) in Iraqi Arab patient with type2 diabetes mellitus (T2DM). Iraqi journal of biotechnology. 2017; 16(3):1-8. https://doi.org/10.25258/ijpqa.v9i01.11358 .
Zakariya BF, Almohaidi AMS, Şimşek SA, Kamal AM, Al-Dabbagh WH, Al-Waysi SA. The whole wheat effect and refined with E-selectin polymorphism on breast cancer. Analytical Science & Technology. 2022; 35(4): 161-8. https://doi.org/10.5806/AST.2022.35.4.161 .
Alzubadiy MW, Almohaidi AMS, Sultan AA, Abdulhameed LQ. Evaluation of E-selectin rs 5367 C/T Polymorphism in Iraqi Diabetic Foot patients. In Journal of Physics: Conference Series 2019; 1294 (6): 06 2021. IOP Publishing. https://doi.org/10.1088/1742-6596/1294/6/062021 .
Cho SB. Molecular Mechanisms of Endometriosis Revealed Using Omics Data. Biomedicines 2023; 1(11) 2-19. https://doi.org/10.3390/biomedicines11082210 .
Rayat S, Ramezanidoraki N, Kazemi N, Mohammad HM, Falah M, Zardadi S and Morovvati S. Association study between polymorphisms in MIA3, SELE, SMAD3 and CETP genes and coronary artery disease in an Iranian population. BMC Cardiovascular Disorders 2022; 22(298) 1-9. https://doi.org/10.1186/s12872-022-02695-6
Yousif RA, Almohaidi AMS, Al-Musawi BJ. Assessment of ITGB3 gene expression and hormonal status in infertile female undergoing an in vitro fertilization protocol. HIV Nurs. 2023; 23(1): 770-8. https://doi.org/10.31838/hiv23.01.130
Elnaggar A, Farag AH, Gaber ME, Hafeez MA, Ali MS, Atef AM. AlphaVBeta3 Integrin expression within uterine endometrium in unexplained infertility: a prospective cohort study. BMC Women's Health. 2017; 17(1): 1-9. https://doi.org/10.1186/s12905-017-0438-3 .
Wang B, Sheng JZ, He RH, Qian YL, Jin F, Huang HF. High expression of l‐selectin ligand in secretory endometrium is associated with better endometrial receptivity and facilitates embryo implantation in human being. Am J Reprod Immunol. 2008; 60(2): 127-34. https://doi.org/10.1111/j.1600-0897.2008.00604.x .
Zakariya BF, Almohaidi AMS, Şimşek SA, Al-Waysi SA, Al-Dabbagh WH, Kamal AM. The relationship of E-26 selectin single nucleotide polymorphisms with breast cancer in Iraqi Arab women. Genomics Inform. 2022; 20(4). https://doi.org/10.5808/gi.22042 .
Achache H, Revel A. Endometrial receptivity markers, the journey to successful embryo implantation. Hum Reprod Update. 2006; 12(6): 731-46. https://doi.org/10.1093/humupd/dml004 .
.Miller D, Motomura K, Garcia-Flores V, Romero R, Gomez-Lopez N. Innate lymphoid cells in the maternal and fetal compartments. Front Immunol. 2018; 9: 2396. https://doi.org/10.3389/fimmu.2018.02396
Wang B, Sheng JZ, He RH, Qian YL, Jin F, Huang HF. High expression of l‐selectin ligand in secretory endometrium is associated with better endometrial receptivity and facilitates embryo implantation in human being. Am J Reprod Immunol. 2008; 60(2): 12734.https://doi.org/10.1111/j.16000897.2008.00604.x .
Pina Carvalho LF, Hui CY, Agarwal A. Endometriosis and infertility: biomarkers affecting implantation rate. J Obstet Gynaecol. 2013; 8(5): 467-73. https://doi.org/10.1586/17474108.2013.825456
Feng Y, Ma Z, Deng L, Yao B, Xiong Y, Wu Y, Wang L, Ma Q, and Ma F. Role of selectins and their ligands in human implantation stage. Glycobiolog. 2017; 27(5) 385–391 https://doi.org/10.1093/glycob/cwx009 .
Vazgiourakis Vm, Zervou2 Mi, Papageorgiou L, Chaniotis D, Spandidosm Da, Vlachakis D,Eliopoulos E and Goulielmos2 Ga. Association of endometriosis with cardiovascular disease: Genetic aspects. International Journal of Molecular Medicine. 2023; 51(29) 1-16. https://doi.org/10.3892/ijmm.2023.5232 .
Ersuz R, Karapınar O S, Doğan S. Comparison of Serum Levels of Cell Adhesion Molecules (E-selectin, P-selectin, Icam-1, Vcam-1, Lrg-1) in Placental İnvasi̇on and Adhesion Anomalies With Patients With Vaginal Delivery and Former Cesarerean. Research Square; 2-19. https://doi.org/10.21203/rs.3.rs-2059755/v1
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