Spectrophotometric Determination of Some Adrenergic Drugs Using H2O2 and Dichlorophenolindophenol


  • Mohamed Y. Dhamra Department of Chemistry, College of Education for Pure Science, University of Mosul, Mosul, Iraq. https://orcid.org/0000-0002-4816-3904
  • Mohammed salim Al-Enizzi Department of Chemistry, College of Education for Girls, Mosul University, Mosul, Iraq.
  • Reem A. Al-Luhaiby Department of Medical Laboratory Techniques, Al-Hadba University College, Mosul, Iraq.




adrenaline hydrochloride, catalytic response, dopamine hydrochloride, salbutamol sulfate, Spectrophotometric


Based on the catalytic response between hydrogen peroxide and dichlorophenolindophenol, a spectrophotometric method was developed to estimate adrenaline hydrochloride  (ADR), Salbutamol sulfate (sal), and dopamine hydrochloride (DOPA) in pharmaceutical preparations. A calculated value of the oxidizing agent (hydrogen peroxide) was added to the basic medium in the presence of dichlorophenolindophenol to form a blue color, which suffers from a bleaching process due to the oxidation process of the drugs mentioned above by the oxidizing agent at λmax 552 nm. The estimated amounts of adrenaline hydrochloride and Salbutamol sulfate were between 0.25 - 5 μg/ml and 0.5 - 5 μg/ml, respectively. The amount of dopamine hydrochloride was between 1 - 7 μg/ml with good sensitivity, as the molar absorbance was 3.8023×104, 8.3506×104and 2.7213×104, respectively. All other statistical values were thoroughly examined. Good agreement with certified value was observed.



Greene BH, Lalonde DH, Seal SK. Incidence of the “adrenaline rush” and vasovagal response with local anesthetic injection. Plast Reconstr Surg Glob Open 2021; 9(6) :1-5. https://doi.org/10.1097/GOX.0000000000003659.

Filogonio R, Crossley DA. Long term effects of chronic prenatal exposure to hypercarbia on organ growth and cardiovascular responses to adrenaline and hypoxia in common snapping turtles. Comp Biochem Physiol A Mol Integr Physiol 2019; 234: 10-7. https://doi.org/10.1016/j.cbpa.2019.04.009

Tschopp C, Tramèr MR, Schneider A, Zaarour M, Elia N. Benefit and harm of adding epinephrine to a local anesthetic for neuraxial and locoregional anesthesia: a meta-analysis of randomized controlled trials with trial sequential analyses. Anesth Analg. 2018; 127(1): 228-39. https://doi.org/10.1213/ANE.0000000000003417.

Douros K, Moriki D, Sardeli O, Boutopoulou B, Galani A, Papaevangelou V, et al. Assessment and management of asthma exacerbations in an emergency department unit. Allergol. Immunopathol. 2023; 51(1): 74-6. https://doi.org/10.15586/aei.v51i1.720.

Kruizinga MD, Birkhoff WA, van Esdonk MJ, Klarenbeek NB, Cholewinski T, Nelemans T, et al. Pharmacokinetics of intravenous and inhaled salbutamol and tobramycin: An exploratory study to investigate the potential of exhaled breath condensate as a matrix for pharmacokinetic analysis. Br J Clin Pharmacol. 2020; 86(1): 175-81. https://doi.org/10.1111/bcp.14156.

Cassidy CM, Zucca FA, Girgis RR, Baker SC, Weinstein JJ, Sharp ME, et al. Neuromelanin-sensitive MRI as a noninvasive proxy measure of dopamine function in the human brain. Proc Natl Acad Sci. 2019; 116(11): 5108-5117. https://doi.org/10.1073/pnas.180798311.

Demkiv O, Stasyuk NY, Gayda G, Grynchyshyn N, Novikevuch O, Demchuk O, et al. A new spectrophotometric method analysis of adrenaline in pharmaceuticals based on laccase-like nanozymes. LNUVMB Vet Sci 2022; 24(106): 142-8. https://doi.org/10.32718/nvlvet10622.

Jabar FM, Al-Sabha TaN, Ismael SO. Spectrophotometric Determination of Salbutamol and Terbutaline using 9-Chloroacridine Reagent. Egypt J Chem. 2022; 65(2): 61-70. https://doi.org/10.21608/EJCHEM.2021.80175.3967.

Al Abdali ZZ, Habeeb NN, Salih ES. Spectrophotometric Determination of Salbutamol Sulphate and Isoxsuprine Hydrochloride in Pharmaceutical Formulations. Baghdad Sci J. 2023; 20(2): 0262-. https://doi.org/10.21123/bsj.2022.6902.

Hamoudi A T, Spectrophotometric assay of salbutamol sulphate in pharmaceutical preparations by coupling with diazotized ρ-bromoaniline. Baghdad Sci J. 2019; 16(1): 610-615. https://doi.org/10.21123/bsj.2019.16.3.0610.

Dhamra MY, Theia’a N, salim Al-Enizzi M. Spectrofluorimetric determination of adrenaline and dopamine. J Sci Educ. 2022; 31(3): 17-26. https://doi.org/10.33899/edusj.2022.133845.1238.

Pandya HN, Berawala HH, Khatri DM, Mehta PJ. Spectrofluorimetric estimation of salbutamol sulphate in different dosage forms by formation of inclusion complex with β-cyclodextrin. Pharm methods. 2010; 1(1): 49-53. https://doi.org/10.1016/S2229-4708(10)11007-3.

Khot G, Kaboli M, Celikel T, Shirtcliffe N. Electrochemical detection of adrenaline and hydrogen peroxide on carbon nanotubes. Surf Innov. 2022; 10(6): 379-86. https://doi.org/10.1680/jsuin.21.00038.

Rajaji U, Chinnapaiyan S, Chen S-M, Govindasamy M, Alothman AA, Alshgari RA. Bismuth telluride decorated on graphitic carbon nitrides based binary nanosheets: Its application in electrochemical determination of salbutamol (feed additive) in meat samples. J Hazard Mater 2021; 413: 125265. https://doi.org/10.1016/j.jhazmat.2021.125265.

Tran TTT, Doan MD, Dinh QK. Differential pulse voltammetry determination of salbutamol using disulfite tungsten/activated carbon modified glassy carbon electrode. Chemosphere. 2022; 303: 135202. https://doi.org/10.1016/j.chemosphere.2022.135202.

Britto‐Júnior J, Antunes NJ, Campos R, Sucupira M, Mendes GD, Fernandes F, et al. Determination of dopamine, noradrenaline, and adrenaline in Krebs–Henseleit solution by liquid chromatography coupled with tandem mass spectrometry and measurement of their basal release from Chelonoidis carbonaria aortae in vitro. Biomed Chromatogr. 2021; 35(2): e4978. https://doi.org/10.1002/bmc.4978.

Jouyban A, Farajzadeh MA, Khoubnasabjafari M, Jouyban-Gharamaleki V, Mogaddam MRA. Derivatization and deep eutectic solvent-based air–assisted liquid–liquid microextraction of salbutamol in exhaled breath condensate samples followed by gas chromatography-mass spectrometry. J Pharm Biomed Anal. 2020; 191: 113572. https://doi.org/10.1016/j.jpba.2020.113572.

Chan W. Investigation of the chemical structure and formation mechanism of polydopamine from self‐assembly of dopamine by liquid chromatography/mass spectrometry coupled with isotope‐labelling techniques. Rapid Commun Mass Spectrom. 2019; 33(5): 429-36. https://doi.org/10.1002/rcm.8373.

Chang S, Ismail A, Daud Z. Ascorbic acid: properties, determination and uses. In: Encyclopedia of Food and Health. Academic Press, London, United Kingdom,. 2016 ; 16(1): 275-284. https://doi.org/10.3390/cosmetics3040038.

Prodromidis M, Stalikas C, Veltsistas PT, Karayannis M. Spectrophotometric kinetic determination of copper (II) trace amounts based on its catalytic effect on the reaction of the reduced 2, 6-dichlorophenolindophenol and hydrogen peroxide. Talanta. 1994; 41(10): 1645-9. https://doi.org/10.1016/0039-9140(94)E0090-E.





How to Cite

Spectrophotometric Determination of Some Adrenergic Drugs Using H2O2 and Dichlorophenolindophenol. Baghdad Sci.J [Internet]. [cited 2024 May 18];21(11). Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/8911