Study the Effect of Alum Activated by Cold Plasma on Mice Wounds Using Textural Analysis

Authors

  • Neean F. Majeed Department of Physics, College of Science for Women, University of Baghdad, Baghdad, Iraq.
  • Alyaa H. Ali Department of Physics, College of Science for Women, University of Baghdad, Baghdad, Iraq. https://orcid.org/0000-0001-6960-023X
  • Sabah N. Mazhir Department of Physics, College of Science for Women, University of Baghdad, Baghdad, Iraq.
  • Rasha S. Akram Department of Physics, College of Science for Women, University of Baghdad, Baghdad, Iraq.

DOI:

https://doi.org/10.21123/bsj.2024.9026

Keywords:

Gray tone spatial-dependence matrices, Plasma, Potassium alum, Textural analysis, XRF analysis.

Abstract

The objective of this research is to concentrate on the gray-tone spatial-dependence matrices to extract the texture features of the wounded skin treated with an alum solution activated with plasma. This method depends on the manual selection of the region of interest, which results in the dependence of parameter values on the extracted region. Potassium alum is used to clean and treat wounds, as well as to prevent bacteria from spreading, where the aqueous solution has aluminum hydroxide which forms a white precipitate on the wounds that works to coagulate the blood and stop bleeding, its efficiency increases when activated by plasma. A group of mice were used; their ages ranged from 2–3 months and their weight ranged from 200–250 g. Wounds were created in mice, and the experiment included using alum in the form of powder, an aqueous solution, and an aqueous solution activated with plasma for a duration of 5 minutes. The textural analysis investigated the effect of alum powder and alum solution on wounds and skin. According to the results of the textural analysis, the histopathology image and gray-tone spatial-dependence matrices, the skin regained its external appearance (near normal), and the wounds healed quickly as a result of employing the plasma-activated aqueous solution. The results were investigated from the histopathology images. 

References

Michelsen K P. Wounds - from physiology to wound dressing. Dtsch Arztebl Int. 2008; 105 (13): 239-48. https://doi.org/10.3238/arztebl.2008.0239.

Hogen H, Esch O, Hagan DT, Fox CB. Optimizing the utilization of aluminum adjuvants in vaccines: you might just get what you want. npj Vaccines. 2018; 3(51): 1- 11. https://doi.org/10.1038/s41541-018-0089-x

Hogenesch H, Mechanism of immuno potentiation and safety of aluminum adjuvants. Front Immunol. 2013; 10(3): 406. https://doi.org/10.3389/fimmu.2012.00406.

Glenny A, Pope CG, Waddington H, Wallace U. Immunological notes. XVI1–XXIV. J Pathol Bacteriol. 1926; 29:31–40. https://doi.org/10.1002/path.1700290106.

Wen Y, Shi Y. Alum: an old dog with new tricks. Emerg Microbes Infect. 2016; 23(5): e25. https://doi.org/10.1038/emi.2016.40.

Hussein A A. The effects of different concentration of Alum solutions on plaque and bleeding levels. J Pharm Sci Res. 2019; 11(3): 1078-1081.

Oryan A, Alemzadeh E. Moshiri A. Burn wound healing: Present concepts, treatment strategies and future directions. J Wound Care. 2017; 26, 5–19 . https://doi.org/10.12968/jowc.2017.26.1.5 .

Mariyam R. Wajeeha B. A Review on Medical Aspect of Alum in Unani medicine and Scientific Studies. World J Pharm Res. 2015; 4(6): 929-940.

Akhtar A, Hamiduddin M, Zaigham M. Shibb-e-yamani (alum) a unique drug and its utilization in unani medicine: A physicochemical and pharmacological review. Int J Res Ayurveda Pharm. 2017; 8 (2): 17-22. http://dx.doi.org/10.7897/2277-4343.08255.

Sawarkar P. Sawarkar G. Management of Chronic burn wound by compound Ayurvedic preparation Dagdhahar Malahar. Ayuriog NJ-RAS. 2018; 6(4): 1-11. http://dx.doi.org/10.52482/ayurlog.v6i04.137.

Chima N, Amadi LO, Ugboma CJ. Antimicrobial Sensitivity Profile of Mimosa pudica Leaf Extract and its Combination Treatment with Potassium Aluminum Sulphate on Some Bacteria. South Asian J Res Microbiol. 2022; 14(1): 36-45. https://doi.org/10.9734/sajrm%2F2022%2Fv14i1264.

National Center for Biotechnology Information. PubChem Compound Summary for CID 24856, Aluminum potassium sulfate. 2023.

Rafieian N, Abdolsamadi H, Moghadamnia A, Jazayeri M, Seif-Rabiee M, Salmanzadeh M, Radi S. Efficacy of alum for treatment of recurrent aphthous stomatitis. Caspian J Intern Med. 2016; 7(3): 201-205.

Olatomirin O K, Wenbin J, Charbel T, Kenneth D G, Daniel G B. Shear Effects on Aluminum Phosphate Adjuvant Particle Properties in Vaccine Drug Products. J Pharmaceutical Sci. 2015; 104(2); 378-387. https://doi.org/10.1002/jps.24127.

Britannica, The Editors of Encyclopaedia. "astringent". Encyclopedia Britannica. https://www.britannica.com/science/astringent.2023 .

Helmboldt O, Hudson L, Chanakya M, Wefers K, Wolfgang H. Aluminum compounds, inorganic. Ullmann's Encyclopedia of Industrial Chemistry (electronic ed.). Weinheim, DE: Wiley-VCH. 2007; https://doi.org/10.1002/14356007.a01_527.pub2.

Orr M T, Khandhar A P, Seydoux E. Reprogramming the adjuvant properties of aluminum oxyhydroxide with nanoparticle technology. npj Vaccines 2019; 4 (1):1-10 https://doi.org/10.1038/s41541-018-0094-0.

Kwok P Y, Darrell G S, Cliff T J, Stanley L H, Aluminum hydroxide adjuvant produced under constant reactant concentration, J Pharm Sci. 2006; 95(8): 1822-1833. https://doi.org/10.1002/jps.20692.

Chaush S, Husain N, Khalid M. The efficacy of alum sitz baths followed by topical gall ointment in hemorrhoids—a single-arm clinical trial. Adv Trad Med (ADTM) 2022; 23: 56-573 https://doi.org/10.1007/s13596-022-00634-6.

APHA. Standard Methods for the Examination of Water and Wastewater. 23rd Ed.. Washington DC: American Public Health Association 2017.

Noori A S, Majeed N F, Abdalameer N K, The histological effect of activated Aloe Vera extract by microwave plasma on wound healing. Chem Phys Lett. 2022; 807: 140112. https://doi.org/10.1016/j.cplett.2022.140112

Khalaf M K, Taha S K, Mohsin H K. Measurement of plasma electron temperature and density by using different applied voltages and working pressures in a magnetron sputtering system. Int J Eng Technol. 2018; 7 (3): 1177-1180. https://doi.org/10.1142/S0219581X22500211.

Majeed NF, Naeemah M R, Ali AH. Spectroscopic analysis of clove plasma parameters using optical emission spectroscopy. Iraqi J Sci. 2021; 62(8): 2565–2570. https://doi.org/10.24996/ijs.2021.62.8.9.

Taha S K. Harb N H, Khalaf M K. A Study of Plasma parameters in gold sputtering System by Means of Optical Emission Spectroscopy. IOP Conf Seri.: Mater Sci Eng. 2020; 871(1): 012081. http://dx.doi.org/10.1088/1757-899X/871/1/012081.

Ali A H, Shakir Z H, Mezhir A. Influence of Cold Plasma on Sesame Pasteand the Nano Sesame PasteBased on Co-occurrence Matri, Baghdad Sci J. 2022; 19(4): 855-864. http://dx.doi.org/10.21123/bsj.2022.19.4.0855.

Muryoush A Q, Ali A H. Effect of Cold Plasma on Histological Compositions of the Rabbits Fracture Bone Tissue. Iraqi J Sci. 2019; 6(9): 1999-2007. https://doi.org/10.24996/ijs.2019.60.9.12.

Abd Al-hadi S, Ali A H, Naeemah M R, Mazher AN. Classification of Brain Lesion using K- Nearest Neighbor technique and Texture Analysis. J Phys Conf Ser. 2019; 1178: 012018. https://doi.org/10.1088/1742- 6596/1178/1/012018.

Mohammed H. Abdullah, Ali A. Hussein. Kidney failure treatment using extract of nano Indian costus prepared by microwave plasma and laser. AIP ConferenceProceedings2024;3036(1).

Downloads

Issue

Section

article

How to Cite

1.
Study the Effect of Alum Activated by Cold Plasma on Mice Wounds Using Textural Analysis. Baghdad Sci.J [Internet]. [cited 2024 Oct. 13];22(1). Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/9026