Hepatocellular Carcinoma Prediction and early Diagnosis of Hepatitis B and C viral infection using miR-122 and miR-223 in a sample of Iraqi patients.

Main Article Content

Saja Khudhier Essa Al-Juboori
https://orcid.org/0009-0001-2253-9993
Shaymaa I K Al-Juboori

Abstract

Hepatocellular carcinoma (HCC) is the third most common cause of cancer-related death. Therefore, it is critical for researchers to understand molecular biology in greater depth.  In several diseases including cancer, abnormal miRNA expression has been linked to apoptosis, proliferation, differentiation, and metastasis. Many miRNAs have been studied in relation to cancer, including miR-122, miR-223, and others. Hepatitis B and C viruses are the most important global risk factors for HCC. This study is intended to test whether serum miRNAs serve as a potential biomarker for both HCC and viral infections HBV and C. The expression of miRNA in 64 serum samples was analyzed by RT-qPCR. Compared to healthy volunteers, HCC patients' sera expressed significantly lower levels of miR-122 and miR-223. Furthermore, we compared the expression of these miRNAs between early-diagnosed HCC patients and healthy controls. There was a significant difference between miR-122 expression in HCC sera and healthy volunteers' sera (0.000 and 0.253, respectively), with a P value of <0.0001. Early diagnostic patients without treatment had completely deleted miR-122 expression levels, while those treated had slightly elevated levels. Clearly, miR-122 has been identified as a biomarker for early detection and follow-up of HCC treatment. HBV and HCV specimens expressed significantly lower levels of miRNA than normal samples with a P value of <0.0001. It is recommended that these findings be further investigated for diagnostic purposes. Further, these miRNAs are highly specific for diagnosing HCC, HBV, and HCV, making them valuable therapeutic indicators.

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1.
Al-Juboori SKE, Al-Juboori SIK. Hepatocellular Carcinoma Prediction and early Diagnosis of Hepatitis B and C viral infection using miR-122 and miR-223 in a sample of Iraqi patients. . Baghdad Sci.J [Internet]. 2023 Aug. 30 [cited 2023 Oct. 4];20(4(SI):1520. Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/9065
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Special Issue - Current advances in anti-infective strategies

References

Zhang C, Cheng Y, Zhang S, Fan J, International QGL. Changing epidemiology of hepatocellular carcinoma in Asia‏. Wiley Interdiscip Rev. 2022; Aug 1 [cited 2023 May 11]; 42(9): 2029-2041. https://onlinelibrary.wiley.com/doi/abs/10.1111/liv.15251

Ogunwobi Olorunseun O, Harricharran T, Huaman J, Galuza A, Odumuwagun O, Tan Yin, et al. Mechanisms of hepatocellular carcinoma progression. World J Gastroenterol. 2019; 25(19): 2279. https://doi.org/10.3748/wjg.v25.i19.2279

Yonezawa M, Yoshida Y, Takikawa Y. Circular microRNA profiles as biomarkers for early-stage hepatocellular carcinoma in patients infected with hepatitis B virus: a pair-matched case-control study. J Iwate Med Assoc. 2020; 72(2):59-67.‏ https://doi.org/10.24750/iwateishi.72.2_59

Abdulla I M, Goreal A A. Detection of Hepatitis-B virus genotypes among chronic carriers in Duhok-Iraq. J Fac Med Baghdad. 2016; 58(2): 170-175.‏ https://www.iasj.net/iasj/download/e16ced60c963c226

Hussein SA, Al-Azzawi RH, Alshareef DQ. Assessment of hepatitis C viral load and genotypes by molecular determination in Iraqi patients treated with Ledipasvir/sofosbuvir (Harvoni) drug. Baghdad Sci J. 2021; 62(5):1431-1437. https://doi.org/10.24996/ijs.2021.62.5.5

Shamas S, Zafar M, Irum S, Khan L, Sadia H, Roshan S, et al. Prevalence of diabetes mellitus in Hepatitis C patients in wazirabad tehsil of gujranwala district of pakistan. Baghdad Sci J. 2020 Dec 1; 17(4): 1154-1154. http://dx.doi.org/10.21123/bsj.2020.17.4.1154

Al-Heety R A, Al-Hadithi H S. Association of Circulating MicroRNA-142-3p with Graves’ Disease. Baghdad Sci J. 2021; 18(4): 1133-1133. http://dx.doi.org/10.21123/bsj.2021.18.4.1133

Faramin Lashkarian M, Hashemipour N, Niaraki N, Soghala S, Moradi A, Sarhangi S, et al. MicroRNA-122 in human cancers: from mechanistic to clinical perspectives. Cancer Cell Int. 2023; 23(1): 1-25. https://doi.org/10.1186/s12935-023-02868-z

Cione E, Abrego Guandique D M, Caroleo M C, Luciani F, Colosimo. Liver Damage and microRNAs: An Update. Curr Issues Mol Biol. 2022; 45(1): 78-91. https://doi.org/10.3390/cimb45010006

Gu J, Xu H, Chen Y. miR-223 as a regulator and therapeutic target in liver diseases. Front Immunol. 2022; 13: 860661. https://doi.org/10.3389/fimmu.2022.860661

Rao X, Huang X, Zhou Z, Lin X. An improvement of the 2ˆ(-delta delta CT) method for quantitative real-time polymerase chain reaction data analysis. Biostat Bioinforma Biomath. 2013 Aug; 3(3): 71-85. PMID: 25558171; PMCID: PMC4280562.

Wischhusen J C, Chowdhury S M, Lee T. Ultrasound-mediated delivery of miRNA-122 and anti-miRNA-21 therapeutically immunomodulates murine hepatocellular carcinoma in vivo. J Control Release. 2020; 321: 272-284.‏ https://doi.org/10.1016/j.jconrel.2020.01.051

Mahmoudian-Sani M R, Asgharzade S, Alghasi A, Saeedi-Boroujeni. MicroRNA-122 in patients with hepatitis B and hepatitis B virus-associated hepatocellular carcinoma. J Gastrointest Cancer. 2019; 10(4): 789.‏ https://doi.org/10.21037/jgo.2019.02.14

Song C I, Lv J, Liu Y. Associations between hepatitis B virus infection and risk of all cancer types. JAMA Netw Open. 2019 2(6): e195718-e195718. https://doi.org/10.1001/jamanetworkopen.2019.5718

Pratedrat P, Chuaypen N, Nimsamer P. Diagnostic and prognostic roles of circulating miRNA-223-3p in hepatitis B virus–related hepatocellular carcinoma. PloS one. 2020; 15(4): e0232211. https://doi.org/10.1371/journal.pone.0232211

Yuan S, Wu Q, Wang Z. miR-223: An immune regulator in infectious disorders. Front Immunol. 2021; 12: 781815.‏ Volume 12 – 2021. https://doi.org/10.3389/fimmu.2021.781815

Casanova M, Pontis F, Ghidotti P. MiR-223 Exclusively Impairs In Vitro Tumor Growth through IGF1R Modulation in Rhabdomyosarcoma of Adolescents and Young Adults. Int J Mol Sci. 2022; 23(22): 13989.‏ https://doi.org/10.3390/ ijms232213989

Dong Y W, Wang R, Cai Q Q. Sulfatide epigenetically regulates miR-223 and promotes the migration of human hepatocellular carcinoma cells. J Hepatol. 2014; 60(4): 792-801.‏ https://www.sciencedirect.com/science/article/pii/S0168827813008684

Ye D, Zhang T, Lou G. Role of miR-223 in the pathophysiology of liver diseases. Exp Mol Med. 2018; 50(9): 1-12. https://doi.org/10.1038/s12276-018-0153-7

Aziz F, Chakraborty A, Khan I, Monts J. Relevance of miR-223 as potential diagnostic and prognostic markers in cancer. J Biol. 2022; 11(2): 249. https://doi.org/10.3390/biology11020249