Phytochemical Analysis of the Ethanol Extract of Binahong (Anredera cordifolia (Ten.) Steenis) Leaves by UV-Vis Spectroscopy

Authors

  • Rusdiana Rusdiana Department of Biochemistry, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia. https://orcid.org/0000-0002-7308-9291
  • Tri Widyawati Department of Pharmacology, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia.
  • Dina Keumala Sari Department of Nutrition, Faculty of Medicine, Universitas Sumatera Utara, Medan Indonesia.
  • Sry Suryani Widjaja Department of Biochemistry, Faculty of Medicine, Universitas Sumatera Utara, Medan, Indonesia.

DOI:

https://doi.org/10.21123/bsj.2024.9354

Keywords:

Anredera cordifolia, Anti-obesity, Flavonoid, Quercetin, Spectrophotometer

Abstract

Binahong (Anredera cordifolia) leaves contain chemical compounds that can be used to promote anti-obesity. This study aimed to examine the phytochemical and anti-obesity properties of the ethanol extract of binahong leaves. The binahong extract was obtained by maceration of the leaves, followed by ethanol extraction. Phytochemical analysis was performed by UV-Vis spectroscopy to identify and determine the levels of flavonoids, phenolic compounds, alkaloids, and tannins in the extract, using quercetin as a standard for calibration. Absorbance was measured at a wavelength of 430 nm. The flavonoid, glycoside, tannin, saponin, and steroid/terpenoid contents were determined, but alkaloid compounds were not found. The conclusion is, that the large flavonoid content (14.9385 mgQE/g) in the binahong leaf extract has potential to provide anti-obesity effects.

References

Alba TM, Pelegrin CM, Sobottka AM. Ethnobotany, ecology, pharmacology, and chemistry of Anredera cordifolia(Basellaceae): a review. Rodriguesia. 2020;13; 71. https://doi.org/10.1590/2175-7860202071060

Cahyaningsih R, Magos Brehm J, Maxted NI. Gap analysis of Indonesian priority medicinal plant speciesaspartoftheir conservation planning. Glob Ecol Conserv 2021; 26: e01459. https://doi.org/10.1016/j.gecco.2021.e01459

Hasanah Y, Lisa M. Exploration and identification of Anredera cordifolia morphological characters in the highlands and lowlands. Biodiv. 2020; 21(6) : 2759-2766. https://doi.org/10.13057/biodiv/d210652.

Rusdiana R, Hamid R Z, Widjaja SS, Fujiati II, Ilyas S, Munir D, et al . Potential Benefits of Ethanol extract of Anredera cordifolia for Antiobesity of High Fat diet-Induced Obesity in White Male Rat Wistar. Baghdad.Sci J. 2023; online first 3. https://doi.org/10.21123/bsj.2023.8666

Leliqia N P E, Sukandar EY, Fidrianny I RDA. Antibacterial activities of Anredera cordifolia (Ten.) v. Steenis leaves extracts and fractions. Asian J Pharm Clin Res .2017; 10 (12) : 10-13. https://doi.org/10.22159/ajpcr.2017.v10i12.21503

Nasution NA, Artika I M, Safari D. Antibacterial activity of leaf extracts of Anredera cordifolia (Ten.) steenis and Muntingia calabura L. against Streptococcus pneumoniae. Curr Biochem. 2020; 7(1): 1-9. https://doi.org/10.29244/cb.7.1.1

Sulfianti A, Firdausi N, Nurhadi N, Ngatinem N, Agustini K, Ningsih S. Antidiabetic activity of Anredera cordifolia (Ten.) Stennis extracts with different ethanol percentages: an evaluation based on in vitro, in vivo, and molecular studies. Pharmacia.2023; 70(1): 39-47. 7. https://doi.org/10.3897/pharmacia.70.e94899.

Suliska N, Suryani Insanu M, Sukandar EY. Antihypertensive Activity of Combination of Anredera cordifolia (Ten.) V. Steenis and Sonchus arvensis L. Leaves on Epinephrine Induced Male Wistar Rat. J Adv Pharm Technol Res. 2021; 12(4): 384–388. https://doi.org/10.4103/japtr.japtr_91_21

Dwitiyanti D, Harahap Y, Elya B, Bahtiar A. Binahong (Anredera cordifolia (Tenore) Steen.) Leaf Extract Modulates Fatty Acids and Amino Acids to Lower Blood Glucose in High-Fat Diet-Induced Diabetes Mellitus Rats. Adv Pharmacol Pharm Sci. 2021; 28: 8869571. https://doi.org/10.1155/2021/8869571.

Feriyani F, Darmawi D, Balqis U, Lubis RR. The Analysis of Binahong Leaves Potential (Anredera cordifolia) as an Alternative Treatment of Anticataractogenesis. Open Access Maced J Med Sci. 2020;8: 820-824. https://doi.org/10.3889/oamjms.2020.4849

Mali, Priti S., and Pradyuman Kumar. Optimization of Microwave Assisted Extraction of Bioactive Compounds from Black Bean Waste and Evaluation of Its Antioxidant and Antidiabetic Potential in Vitro. Food Chem Adv. 2023;3: p. 100543, https://doi.org/10.1016/j.focha.2023.100543

Omer GA., Mohammed LY. Total Phenolic, Flavonoids and Vitamin C Contents with Antioxidant Activity of Urtica dioica L. Leaves Growing in Zakho, Kurdistan Region-Iraq. Baghdad Sci J.2023; online first 5 https://doi.org/10.21123/bsj.2023.8658

Firdaus MNA, Kardela W, Ifora I. Phytochemical and Anti-Inflammatory Potential of Anredera cordifolia (Ten): A Review. J Drug Deliv Ther.2022; 12 (2), 121-125. https://doi.org/10.22270/jddt.v12i2.5228

Retno M, Endang K, Elena NN. Areca catechu L. and Anredera cordifolia (Ten) Steenis supplementation reduces faecal parasites and improves caecal histopathology in laying hens. Int J Vet Sci Med. 2022; 10:1: 52-63. https://doi.org/10.1080/23144599.2022.2090732

Dwitiyanti, Harahap Y, Elya B, Bahtiar A. Impact of solvent on the characteristics of standardized binahong leaf (Anredera cordifolia (Ten.) Steenis). Pharmacogn J. 2019; 11(6): 1463-1470. http://www.phcogj.com/v11/i6s.

Yuniarti WM, Lukiswanto BS. Effects of herbal ointment containing the leaf extracts of Madeira vine (Anredera cordifolia (Ten.) Steenis) for burn wound healing process on albino rats. Vet world. 2017;10 (7): 808. https://doi.org/10.14202/vetworld.2017.808-813.

Souhoka FA, Kapelle IBD, Sihasale E. Phytochemical and antioxidant test of binahong (Anredera cordifolia (Tenore) Steenis) leaves ethanol extract. Fullerene Journal of Chemistry. 2021; 6 (1): 28-33. https://doi.org/10.37033/fjc.v6i1.248

Salim A, Dandy FK, Felicia S, Janice ES, Putri AJ, Tiara A, et al. Phytochemical Screening and Therapeutic Effects of Binahong (Anredera cordifolia (Ten.) Steenis) Leaves. Indonesian journal of life sciences. 2021; 43-55. https://doi.org/10.54250/ijls.v3i2.125

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Phytochemical Analysis of the Ethanol Extract of Binahong (Anredera cordifolia (Ten.) Steenis) Leaves by UV-Vis Spectroscopy. Baghdad Sci.J [Internet]. [cited 2024 May 18];21(11). Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/9354