Evaluation of Antibiofilm and Cytotoxic Activity of Microalgae Isolated from Different Sites of betwata- Erbil/Iraq
DOI:
https://doi.org/10.21123/bsj.2024.9824Keywords:
Antibiofilm, Cell Line, Cytotoxicity, Cell viability, MicroalgaeAbstract
This in vivo study is aimed to assess the antibiofilm and cytotoxic potential of selected microalgae isolates. Different concentration of microalgal isolates used for each of antibiofilm and cytotoxic studies. A total extract of 10 μL MTT was added to each well with LB broth, plates were read using microtiter plate spectrophotometer at 490 nm and 630 nm, respectively. The cell viability was determined in CCD-18℃ cells (3x103 cells/well) by MTT assay at 590 nm. The microalgae isolates showed antibiofilm affect after 48 hrs on Pseudomonas aeruginosa PA01 and this effect was observed to be higher than those of S. aureus. Chlorella sp. which showed the maximum antibiofilm activity while Arthrospira platensis had no biofilm inhibition activity. Chlorella sp. inhibited about 82.67% of the biofilm at 5 mg/mL concentration. Moreover, the antibiofilm effects of Chara sp. and Spyrogyra sp.1 reached 79.01% and 76.31% at 1 mg/mL, respectively. Among all microalgal isolates, a moderate inhibitory effect was observed on Pseudomonas aeruginosa biofilm with Chlorella sorokiniana. Cell viability was not changed significantly at higher doses extracts. More than 73% of the cells were viable in all concentration of the algae extracts. All of the microalgal samples were found to have biofilm inhibition activity. The findings suggested that future development of microalgal samples as a means of inhibiting Pseudomonas aeruginosa biofilms is possible. Colonic epithelial cells may become cytotoxic in response to algae extracts. Further research is necessary to verify that extracts are safe for human consumption, which may have been hypothesized.
Received 02/10/2023
Revised 11/12/2023
Accepted 13/12/2023
Published Online First 20/04/2024
References
Schulze A, Mitterer F, Pombo J P, Schild S. Biofilms by bacterial human pathogens: Clinical relevance - development, composition and regulation - therapeutical strategies. Microb Cell. 2021 Feb; 8(2): 28–56. https://doi.org/10.15698/mic2021.02.741
Prestinaci F, Pezzotti P, Pantosti A. Antimicrobial resistance: A global multifaceted phenomenon. Pathog Glob Health. 2015 Sep; 109(7): 309–318. https://doi.org/10.1179/2047773215Y.0000000030
Schoenborn AA, Yannarell SM, Wallace ED, Clapper H, Weinstein I C, Shank EA. Defining the expression, production, and signaling roles of specialized metabolites during bacillus subtilis differentiation. J Bacteriol. 2021Oct; 203(22): e0033721. https://doi.org/10.1128/JB.00337-21
Karunakaran E, Biggs CA. Mechanisms of bacillus cereus biofilm formation. An investigation of the physicochemical characteristics of cell surfaces and extracellular proteins. Appl. Microbiol Biotechnol. 2011 Feb; 89(4): 1161–1175. https://doi.org/10.1007/s00253-010-2919-2
Shaima AF, Yasin NH, Ibrahim NH, Takrif MS, Gunasekaran DI, Mahmud YY. Unveiling antimicrobial activity of microalgae Chlorella sorokiniana (UKM2), Chlorella sp. (UKM8) and Scenedesmus sp. (UKM9). Saudi J Biol Sci. 2022 Oct; 29(2): 1043–1052. https://doi.org/10.1016/j.sjbs.2021.09.069
Lauritano C, Andersen J, Hansen E, Albrigtsen ME, Esposito F, Khanssen K, et al. Bioactivity screening of microalgae for antioxidant, anti-inflammatory, anticancer, anti-diabetes, and antibacterial activities. Front Mar Sci. 2016 May; 3(68): 1–2. https://doi.org/10.3389/fmars.00068
Barrera DJ, Rosenberg JN, Chiu JG, Chang YN, Debatis M, Ngoi SM, et al. Algal chloroplast produced camelid VH antitoxins are capable of neutralizing botulinum neurotoxin. Plant Biotechnol J. 2015 Jan; 13(1): 117-124. https://doi.org/10.1111/pbi.12244
Tian F, Li J, Nazir A, Tong Y. Bacteriophage - a promising alternative measure for bacterial biofilm control. Infect Drug Resist. 2021 Jan; 20(14): 205–217. https://doi.org/10.2147/IDR.S290093
Arslan A, Yılancıoğlu K, Kuşoğlu S, Albayrak G. Chemical constituent of Isochrysis galbana microalgae extract and its cytotoxic activities on leukemic cell lines. Istanbul J Pharm. 2022 Apr; 52 (1): 64-68. https://doi.org/10.26650/IstanbulJPharm.2022.105733
Anwer S. Direct analysis of algae for determination of major and minor elements using energy dispersive x-ray fluorescence (EDXRF), Kuwait Journal of Science, 2023 Sep; 50 ( 4): 697-702, https://doi.org/10.1016/j.kjs.2023.04.012.
Shen F, Ge C, Yuan P. Metabolomics Study Reveals Inhibition and Metabolic Dysregulation in Staphylococcus aureus Planktonic Cells and Biofilms Induced by Carnosol. Front Microbiol. 2020 Sep; 18; 11: 538572. https://doi.org/10.3389/fmicb.2020.538572
Sivaranjani M, Gowrishankar S, Kamaladevi A, Pandian SK, Balamurugan K, Ravi AV. Morin inhibits biofilm production and reduces the virulence of Listeria monocytogenes an in vitro and in vivo approach. Int J Food Microbiol. 2016 Nov; 21: 237: 73–82. https://doi.org/10.1016/j.ijfoodmicro.2016.08.021
Feng J, Yee R, Zhang S, Tian L, Shi W, Zhang WH, et al A Rapid Growth-Independent Antibiotic Resistance Detection Test by SYBR Green/Propidium Iodide Viability Assay. Front Med. 2018 May; 5(127): 1-11. https://doi.org/10.3389/fmed.2018.00127
Haney EF, Trimble MJ, Cheng JT, Vall Q, Hancock RE W. Critical assessment of methods to quantify biofilm growth and evaluate antibiofilm activity of host defence peptides. Biomolecules. 2018 May; 8(2)29. https://doi.org/10.3390/biom8020029
Sahin C, Mutlu D, Nasirli F, Mahmoudi G, Zubkov FI, Arslan S, et al. New iridium bis-terpyridine complexes: synthesis, characterization, antibiofilm and anticancer potentials. Biometals. 2021 Apr; 34: 701–713. https://doi.org/10.1007/s10534-021-00307-y
David S, Perkins RS, Fronczek FR, Kasiri S, Mandal SS, Srivastava RS. Synthesis, characterization, and anticancer activity of ruthenium-pyrazole complexes. J Inorg Biochem. 2012 Jun;111:33-9.
http:// doi.org/10.1016/j.jinorgbio.2012.02.022.
Nedji N, Loucif-Ayad W. Antimicrobial activity of Algerian propolisin foodborne pathogens and its quantitative chemical composition. Asian Pacific Journal of Tropical Disease, 2014 4(6): 433–437. http://doi.org/10.1016/S2222-1808(14)60601-0
Wu Z, McGoogan JM. 2020. Characteristics of and important lessons from the Coronavirus Disease 2019 (COVID-19) outbreak in China: Summary of a Report of 72 314 Cases From the Chinese Center for Disease Control and Prevention. Jama-Journal of the American Medical Association 2020 Apr323(13): 1239–1242. https://doi.org/10.1001/jama.2020.2648
Nübel U, Garcia-Pichel F, Muyzer G. PCR primers to amplify 16S rRNA genes from cyanobacteria. Appl. Environ. Microbiol. 1997Aug; 63(8): 3327-3332. https://doi.org/10.1128/aem.63.8.3327-3332.1997
Guchhait KC, Manna T, Barai M, Karmakar M, Nandi SK, Jana D, et al. Antibiofilm and anticancer activities of unripe and ripe Azadirachta indica (neem) seed extracts. BMC Complement Med Ther. 2022 Feb; 22(42): 1-18. https://doi.org/10.1186/s12906-022-03513-4
Elshobary E, El-Shenody A, Ashour M, Zabed M, Qi X. Antimicrobial and antioxidant characterization of bioactive components from Chlorococcum minutum. Food Biosci. 2020 Jan; 35: 100567. https://doi.org/10.1016/j.fbio.2020.100567
Anwer SS, Sdiq KH, Muhammad KR, Aladdin LM. Phenolic compound and fatty acid properties of some microalgae species isolated from Erbil City. Braz J Biol. 2022 Jan; 21; 82: e256927. https://doi.org/10.1590/1519-6984.256927
Gadhi A, El-Sherbiny M, Al-Sofyani A, Ba-Akdah M, Satheesh S. Antibiofilm Activities of Extracts Of The Macroalga Halimeda Sp. From The Red Sea. J Mar Sci Technol. 2018; 26(6): 838-846. https://doi.org/10.6119/JMST.201812_26(6).0008
Ibraheem S, Sadiq AS, Al-TameemIi M, Alias MF. Synthesis, Spectral Identification, Antibacterial Evaluation and Theoretical Study of Co, Fe, Rh and Pd Complexes for 2-benzoylthiobenzimidazol. Baghdad Sci J. 2022 Dec; 19(6): 1326-1334. https://dx.doi.org/10.21123/bsj.2022.6704
Griffin C, Karnik A, McNulty J, Pandey S. Pancratistatin selectively targets cancer cell mitochondria and reduces growth of human colon tumor xenografts. Mol Cancer Ther. 2011 Jan; 10(1): 57-68. https://doi.org/10.1158/1535-7163.MCT-10-0735 . PMID: 21220492.
Toma JJ, Aziz F. Antibacterial Activity of Three Algal Genera against some Pathogenic Bacteria. Baghdad Sci J. 2023 Feb; 20(1): 32-40. https://dx.doi.org/10.21123/bsj.20022.6818
Takawa M, Masuda K, Kunizaki M, Daigo Y, Takagi K, Iwai Y, et.al. Validation of the histone methyltransferase EZH2 as a therapeutic target for various types of human cancer and as a prognostic marker. Cancer Sci. 2011; 102(7): 1298-1305. https://doi.org/10.1111/j.1349-7006.2011.01958.x
Mendanha, D.; Vieira de Castro, J.; Moreira, J.; Costa, B.M.; Cidade, H.; Pinto, M.; Ferreira, H.; Neves, N.M. A New Chalcone Derivative with Promising Antiproliferative and Anti-Invasion Activities in Glioblastoma Cells. Molecules 2021, 26, 3383. https://doi.org/10.3390/molecules26113383
Zong A, Cao H, Wang F. Anticancer polysaccharides from natural resources: a review of recent research. Carbohydr Polym. 2012 Nov; 90(4): 1395–1410. https://doi.org/10.1016/j.carbpol.2012.07.026
Mosaddegh M, Gharanjik BM, Naghibi F, Esmaeili S, Pirani A, Eslami Tehrani B, et al. A survey of cytotoxic effects of some marine algae in the Chabahar coast of Oman Sea. Res j pharmacogn. 2014; 1: 27–31.
Bechelli J, Coppage M, Rosell K, Liesveld J. Cytotoxicity of algae extracts on normal and malignant cells. Leuk Res Treatment. 2011 Jan; 2011: 373519. https://doi.org/10.4061/2011/373519
Kurt O, Özdal-Kurt F, Akçora CM, Özkut M, Tuğlu, MI. Neurotoxic, cytotoxic, apoptotic and antiproliferative effects of some marine algae. Extracts on the NA2B cell line. Biotech. Histochem. 2018 January; 93(1): 59–69. https://doi.org/10.1080/10520295.2017.1381992
El-Sheekh M, Abu-Faddan M, Abo-Shady A, Nassar MZA, Labib W. Molecular identification, biomass, and biochemical composition of the marine Chlorophyte Chlorella sp. MF1 isolated from Suez Bay. J Genet Eng Biotechnol. 2020 Jul; 18(1): 27. https://doi.org/10.1186/s43141-020-00044-8
Yarmohammadi S, Hosseini R, Foshati S, Moradi M, Hemati N, Moradi S, et al. Effect of Chlorella vulgaris on Liver Function Biomarkers: a Systematic Review and Meta-Analysis. Clin Nutr Res. 2021 Jan; 10(1):83-94. https://doi.org/10.7762/cnr.2021.10.1.83
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Copyright (c) 2024 Sewgil Saaduldeen Anwer, Ayad Kareem Ali, Nur Bozbeyoglu Kart, Dogukan Mutlu, Refika Ceyda Beram, Volkan Kuzucu, Sevki Arslan, Nazime Mercan Dogan
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