Study of the Toxicity and Cell Viability of Zirconium Oxide Nanoparticles Prepared from an Extract of the Vitex Agnus Castus plant
DOI:
https://doi.org/10.21123/bsj.2024.9881Keywords:
Antibacterial, Biosynthesis approach, Cell line A549, Nanoparticles, Zirconium oxide.Abstract
In the current study, ZrO2 nanoparticles were synthesized using a plant extract derived from Vitex agnus castus, and an alkaline medium such as sodium hydroxide. A biosynthetic approach was utilized to prepare zirconium oxide nanoparticles for this research project. This method stands out from others due to its cost-effectiveness, simplicity, and lack of potential risks.The prepared samples were characterized using transmission electron microscopy TEM, scanning electron microscopy SEM, Fourier transform infrared spectroscopy FT-IR, ultraviolet-visible spectroscopy UV-VIS, X-ray diffraction, and energy-dispersive X-ray spectroscopy EDX. The size of the crystal was determined using X-ray diffraction in conjunction with the Debye-Scherer equation, resulting in a value of 26.37 nm. Scanning electron microscopy and transmission electron microscopy were employed to ascertain the particle size of ZrO2 nanoparticles.In this study, these nanoparticles exhibited varying levels of activity against two types of gram-positive bacteria(Staphylococcus aurous and Streptococcus pneumonia), two types of gram-negative bacteria (Proteus mirabilis and Escharia coli), and one type of fungus, Candida. Interestingly, synthesized zirconium oxide nanoparticles' anticancer potential has been uncovered with MTT assays at varied concentrations for cell line A549 lung cancer. The percentage of inhibition revealed an increase with increasing concentration. Calculating the inhibition of half of the cells IC50, which was equal to (58.4 mg/ml), suggests that zirconium oxide nanoparticles have the potential for utilization in cancer treatment.
Received 07/10/2023
Revised 12/01/2024
Accepted 14/01/2024
Published Online First 20/09/2024
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