دراسة التنوع الوراثي لعدد من أصناف الرز العراقي المستزرع في وسط وجنوب العراق باستخدام تقنية تسلسل الجيل القادم
DOI:
https://doi.org/10.21123/bsj.2024.10073الكلمات المفتاحية:
جينوم البلاستيدة الخضراء، العلاقات التطورية، أوريزا AA جينوم، تحليل شجرة التطور الوراثي، الأرز (أوريزا ساتيفا).الملخص
نظرًا لأهمية محصول الأرز في العراق ، أجريت هذه الدراسة لتحديد أصول الأصناف الرئيسية وفهم العلاقات التطورية بين أصناف الأرز العراقية وغيرها من انواع الأرز الآسيوية التي يمكن أن تكون ذات اهمية كبيرة في تحسين هذا المحصول. تم الحصول على خمسة أنواع من قسم البحوث الزراعية في العراق, ومن بين هذه الاصناف ( العنبر 33 ، دجلة ، غدير ، البركة ، والأرز الأسود) وقد تم تسلسل الحمض النووي الجيني باكملة باستخدام منصات تسلسل الجيل التالي القائمة على تقنية DNA nanoball (DNB),تم الحصول على تسلسل 26 نوعاً من قاعدة بيانات موارد جينوم NCBI .اظهر التحليل التطوري بنائاً على البلاستيدة الخضراء الانواع قسمت الى مجاميع وفقاً الى موقعهم الجغرافي تم تقسيم الأصناف العراقية إلى مجموعتين المجموعة الاولى تحتوي Amber33 و Japonica NC_001320 بينما تحتوي مجموعة ثانية على Dijla و Ghadir و Baraka و Black Rice و Indica NC_008155.
Received 29/10/2023
Revised 02/01/2024
Accepted 04/01/2024
Published Online First 20/08/2024
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