تقدير مادة الكلورفينيرامين ماليات في مستحضراتها الصيدلانية باستخدام محلل الحقن الجرياني العكر محلي الصنع
DOI:
https://doi.org/10.21123/bsj.2024.10244الكلمات المفتاحية:
مضادات الهستامين، كلوروفينيرامين ماليات، محلل CFI ، الحقن الجرياني المستمر، دقائق الراسب، طريقة قياس الطيف الضوئي للأشعة المرئية و فوق البنفسجيةالملخص
باستخدام تقنية التحليل بالحقن الجرياني المستمر: تم في هذه الدراسة تقديم طريقة تحليلية جديدة لتقدير مادة الكلورفينيرامين ماليات (CPM) في صورتها النقية وفي بعض مستحضراتها الصيدلانية. اعتمدت الدراسة على تفاعل CPM مع كبريتات الأمونيوم السيريوم الرباعي ( (ACS في وسط حمضي، حيث نتج عن التفاعل راسب أبيض مصفر قليلاً. تمت دراسة الراسب المتكون باستخدام محلل الخلايا الشمسية محلي الصنع (Ayah 6SX1-ST-2D solar cell CFI analyzer) عن طريق قياس الضوء المنعكس من سطوح دقائق الراسب عند (0-180 درجة). تم إجراء بعض التجارب لدراسة وتحسين بعض المعلمات الكيميائية والفيزيائية. كان معامل الارتباط (r) = 0.9996، و منحنى المعايرة الخطية ضمن المدى (0.07-5 ملمول/لتر). حد الكشف للطريقة المقترحة (L.O.D) هو 195 نانوغرام/10 ميكرولتر. كانت نسبة الانحراف النسبي RSD% أقل من 0.2% للتراكيز 1و0، 5و1 و0و4 مليمول/لتر من CPM تم تكرار القياس لكل تركيز 8 مرات. تم تطبيق الطريقة بنجاح في تقدير CPM في ثلاث عينات من المستحضرات الصيدلانية المتوفرة في سوق الأدوية العراقي لثلاث شركات مختلفة. تمت مقارنة الطريقة الجديدة مع طريقة قياس الطيف الضوئي للأشعة فوق البنفسجية عند طول موجي اعظم 261 نانومتر. وللتأكد من عدم وجود فرق معنوي مهم بين الطريقة المقترحة وطريقة القياس الطيفي، تم إجراء اختبار t واختبار F. وأظهرت نتائج الاختبارين عدم وجود فروق معنوية عند مستوى ثقة 95%، حيث كانت قيمة t المحسوبة 162و2. وهي أقل من قيمة t الجدولية (4.303) وكذلك قيمة F المحسوبة (31.9770) أقل من قيمة F الجدولية.
Received 20/11/2023
Revised 04/03/2024
Accepted 06/03/2024
Published Online First 20/12/2024
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