تقييم مستوياتSOD  و MDA مع السمية الخلوية لبعض المستخلصات النباتية تجاه خطوط خلايا السرطان العضلية البشرية

المؤلفون

  • كاظم خضير غضيب قسم الكيمياء، كلية العلوم للبنات، جامعة بغداد، بغداد، العراق
  • فيحاء مقداد خليل قسم الكيمياء، كلية العلوم للبنات، جامعة بغداد، بغداد، العراق

DOI:

https://doi.org/10.21123/bsj.2024.10992

الكلمات المفتاحية:

انزيم مضاد الاكسدة، مستخلص درنات الزنجبيل، مستخلص اوراق الزيتون، واسم الاكسدة الفوقية، خطوط خلايا الساركوما.

الملخص

حظيت السمية الخلوية للمستخلصات النباتية باهتمام كبير في السنوات الأخيرة لتقييم نشاطها ضد الأورام. تركز الوقاية الكيميائية من السرطان على السمية الخلوية للمستخلصات النباتية لتكون سامة تجاه خطوط الخلايا السرطانية دون أن تؤثر على الخلايا الطبيعية. الهدف من هذه الدراسة هو تقييم السمية الخلوية لمستخلصي الزنجبيل و أوراق الزيتون ومزيجهما بنسبة 1: 1 تجاه خطوط خلايا RD وتقدير فعالية هذه المسخلصات ضد الاجهاد التاكسدي  بدلالة SOD و MDA. تم الحصول على المستخلصات الكحولية لكل من الزنجبيل وأوراق الزيتون بطريقة السوكسيلت. وحضر المستخلص الثالث بخلط كميات متساوية من مستخلص أوراق الزيتون والزنجبيل بنسبة 1: 1. تم تحضير اطباق من خطوط الخلايا RD وخطوط الخلايا الاعتيادية وتم اختبار تراكيز مختلفة من المستخلصات المحضرة (12.5 - 800 ميكروغرام / مل) لتسجيل فعالياتهم تجاه كل من RD وخطوط الخلايا الطبيعية. . كذلك ، تم تقدير  مستوى انزيم  SOD و MDA اعتمادًا على طرائق العمل الخاصة بهما. اظهرت النتائج  وجود فرق معنوي بين السمية الخلوية للتراكيز المدروسة لكل مستخلص تجاه خطوط الخلايا RD، اذ انخفضت قابلية بقاء خطوط الخلايا RD مع زيادة تراكيز المستخلصات ، وفي نفس الوقت لم تؤثر تلك  المستخلصات على خطوط الخلايا الطبيعية. كذلك بينت النتائج ازدياد مستويات SOD مع زيادة تركيز المستخلص على عكس مستويات MDA التي انخفضت مع زيادة تراكيز المستخلص. يمكن الاستنتاج بان نشاط المستخلصات المحضرة تجاه خطوط الخلايا RD دون التأثير على خطوط الخلايا الطبيعية يعزز مبادئ المنع الكيميائي للسرطان ، إلى جانب الخصائص المضادة للأكسدة التي يمكن أن تقلل من الإجهاد التأكسدي الذي يصاحب الخلايا السرطانية  وتنشيط العوامل الطبيعية المضادة للاكسدة في جسم الانسان.   

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كيفية الاقتباس

1.
تقييم مستوياتSOD  و MDA مع السمية الخلوية لبعض المستخلصات النباتية تجاه خطوط خلايا السرطان العضلية البشرية. Baghdad Sci.J [انترنت]. [وثق 21 ديسمبر، 2024];22(4). موجود في: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/10992