التنميط الجزيئي لاثنين من عزلات اللشمانيا الجلدية المشتبه بهما في بغداد
الشريط الجانبي للمقالة
محتوى المقالة الرئيسي
الملخص
يعتبر داء الليشمانيات من الأمراض الطفيلية التي يسببها طفيلي الليشمانيا ، وهو مرض معدي متوطن في العراق. التشخيص بالطرق التقليدية غير دقيق وغير قادر على تشخيص طفيلي الليشمانيا على مستوى النوع. في هذه الدراسة تم التشخيص بطريقتين جزيئية بالتنميط الجزيئي للاطوار المسوطة لداء الليشمانيات الجلدي ، على مستوى النوع. الأول كان تفاعل البلمرة المتسلسل (PCR) للبادئB6 والثاني ITS1-PCR متبوعًا بتعدد الأشكال المقيدة لطول الجزء باستخدام إنزيم التقييد HaeIII. تم استخلاص الحمض النووي من الاطوار المسوطة خارج الجسم الحي متبوعة بتضخيم kDNA والمنطقة ITS1 باستخدام زوج البادئ B6 و LITSR / L5.8S قبل هضم ناتج تضخيم تفاعل البلمرة ITS1 . نواتج تضخيم تفاعل البلمرة كانت bp359 و bp450 لـ B6 و ITS1 ، على التوالي. كشف ITS1-RFLP عن حزميتن متميزتين يتراوح حجمهما بين bp150 و,300 bp عل التوالي. أثبتت هذه النتائج أن العزلتين تعودان إلى داء الليشمانيا الجلدي ، تحديدا النوع الاستوائي. نوصي بالطريقتين الجزيئيتين للفحص المباشر للعينات المأخوذة من قرحة الجلد للمرضى الذين يشتبه في إصابتهم بداء الليشمانيات الجلدي للتشخيص الحساس والسريع لأكثر السلالات العراقية انتشارًا من داء الليشمانيات الجلدي وهي Leishmania tropica.
تفاصيل المقالة
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كيفية الاقتباس
المراجع
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