عقار الكولستين علاج احادي جيد لكبح جماح بكتريا الراكدة البومانية Acinetobacter baumannii خارج وداخل الجسم الحي

محتوى المقالة الرئيسي

Halah H. Al-Haideri
Nagham Saad Mohammed

الملخص

 


تعد بكتيريا Acinetobacter baumannii من احد مسببات الأمراض الانتهازية الرئيسية التي تقاوم العديد من المضادات الحيوية. الكوليستين هو مضاد حيوي يستخدم في الوقت الحاضر كطريقة  لمعالجة أخيرة للعزلات ذات المقاومة الشديدة. الغرض من هذه الدراسة هو التعرف على أهمية  جين LptD في الطبقه الدهنية LPS  من بكتريا A. baumannii ومعرفة دورها  الاساسي كعامل ضراوة.في الدراسة الحالية،  تم استخدام عزلتين من بكترياA.baumannii ، االعزلة المحلية HHR1 والعزلة العالمية ATCC 17904 ، وتم استخدام ثلاث انواع من المضادات الحياتية  (الكوليستين ،الريفامبيسين و  الفوسفوميسين كعلاج احادي او مزدوج خارج وداخل الجسم الحي.اظهرت النتائج أن A. baumannii HHR1 هي أكثر مقاومة لـ AMPs (Antimicrobial peptides) من السلالات العالمية ، تم ملاحظة ازدياد تأثير AMPs على A. baumannii بزيادة التركيز ووقت الحضانة ، كما تبين أيضًا أن AMPs تعتبر قاتله على بكتريا A. baumannii عند التركيز العالي (µg ml-12 ) في العلاج الأحادي و (1.5 ، 2 ) µg ml-1  في الدمج. علاوة على ذلك ، كانت حساسية العزلات متغايرة ، حيث اظهر الكولستين فعالية متميزة في تثبيط النمو البكتيري كعلاج احادي او عند المزج مع مضاد اخر.أظهرت النتائج أن التعبير الجيني لجينات lptD ، lptA للعزله المحلية أقل من العزلة القياسية بتركيز  2 µg ml-1 من الكوليستين ، بينما جين lptE كانت العزله القياسية  ذات  نمط تنظيم أعلى مما كان عليه في العزلة المحلية. علاوة على ذلك ، يؤثر الكوليستين على التصاق  A. baumannii على الخلايا الظهارية (A-549 خلية سرطان الرئة) ، وأظهر الكولستين تأثيرًا كبيرًا على نمو البكتيريا ، وتكاثر الخلايا ، عن طريق تقليل اعداد البكتيريا.

تفاصيل المقالة

كيفية الاقتباس
1.
عقار الكولستين علاج احادي جيد لكبح جماح بكتريا الراكدة البومانية Acinetobacter baumannii خارج وداخل الجسم الحي. Baghdad Sci.J [انترنت]. 1 أبريل، 2022 [وثق 24 نوفمبر، 2024];19(2):0255. موجود في: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/5706
القسم
article

كيفية الاقتباس

1.
عقار الكولستين علاج احادي جيد لكبح جماح بكتريا الراكدة البومانية Acinetobacter baumannii خارج وداخل الجسم الحي. Baghdad Sci.J [انترنت]. 1 أبريل، 2022 [وثق 24 نوفمبر، 2024];19(2):0255. موجود في: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/5706

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