تقدير التركيب الكيميائي للمركبات الثانوية للفطر البري العراقي Agaricus bellaniae وتشخيصه مورفولوجيا وجزئيا
محتوى المقالة الرئيسي
الملخص
هدفت هذه الدراسة التي تعد الأولى من نوعها في العالم والوطن العربي التي أجريت في مختبر أنتاج الفطر التابع لوحدة بحوث النباتات الطبية/ كليةعلوم الهندسة الزراعية– جامعة بغداد للفترة من 21 تموز 2016 ولغاية 30 كانون الأول 2018 إلى عزل وتنقية الغزل الفطري للعزلة البرية ، ثم إجراء التشخيص المظهري والجيني لها ،إذ اظهر ان العزلة البرية تعود للفطر Agaricus bellaniae بعدها تم ترميز العزلة النقية التي تم الحصول عليها في منظمة NCBI الأمريكية بالرمز MF987843.1وبذا يكون العراق ثاني دولة في العالم ينمو فيها الفطر بعد الولايات المتحدة الامريكية ،كما تم تحديد درجات الحرارة المثلى لسرعة نمو الغزل الفطري مختبريا ،إذ تراوحت مابين50 -60◦م ،كما تم اجراء التشخيص النوعي والكمي لمحتوى الاجسام الثمرية المجففة للفطرالبري لمعرفة محتواه من المركبات الفعالة طبيا ،إذ اظهرت ارتفاع نسبة كل من Linoleic acid47.77 % و مضادات الاكسدةالكليةوالفينولاتالكلية والفلافونويدات الكلية ، فضلا عن محتوى الاجسام الثمرية من المركبات الكيمائية الاساسية وخصوصا ارتفاع نسبة البروتين 44% والعناصر المعدنية خصوصا عنصر السلينيوم0.3691ppm والاحماض الامينية والتي سجل كل من حامضي الكلوتاميكوالاسبارتيك نسبة مرتفعة بلغت 4.02 % و2.26 % على التوالي.
Received 6/3/2022
Revised 12/11/2022
Accepted 13/11/2022
Published Online First 20/4/2023
تفاصيل المقالة

هذا العمل مرخص بموجب Creative Commons Attribution 4.0 International License.
كيفية الاقتباس
المراجع
Rukaibaa A C, Ekhlas MF, Mowafaq M M, Ziena M A. Morphological characterization, molecular diagnosis and enzymatic activity of some wild mushroom in Baghdad province, Iraq. Plant Arch. 2020; 20(2): 7437-7445.
Mowafaq M M, Ziena MA, Jasim M A Rukaibaa A C. Collection, morphological characterization, molecular diagnosis and enzymatic activity of some wild mushroom in Ad diwanya province, Iraq. Biochem. Cell. Arch.2020;20(2): 5107-5114
Marthad A S, Shatha A Sh Rukaibaa AC. Production of spawn with high quality from novel Iraqi strains of edible mushrooms.Plant Arch.2020; 20(1):1188-1193.
Ahlavat OP, Manikandan K, Singh M. Proximate composition of different mushroom varieties and effect of UV. light exposure on vitamin D content in Agaricus bisporus and Volvariellavolvacea. Mushroom Res 2016; 25(1): 1-8. https://www.cabdirect.org/cabdirect/abstract/20193144508
Sabri MA, Shatha A Sh ,Rukaibaa AC.Utilization of agricultural and animal wastes in growth of novel Iraqi strains of edible mushrooms Pleurotus Ostreatus and Brown Agaricus bisporus. Plant Arch. .2019; 19(2): 188-1193.
Ekhlas M F, Rukaibaa A C, Ziena MA Modulate Genotoxicity Effects Of Cyclophosphamide By Local P. Ostreatus (Id:Mf065715.1) Extract In Vivo. Biochem Cell Arch. 2018; 18(2): 2419-2425. https://www.researchgate.net/publication/
Rukaibaa A C, Mohammed O M, Ziena M A, Malak M A. Prepration of new national media for cultivation and effect of some environmental factors on growth rate of oyster mushroom. Iraqi J Agric Sci. 2017; 48(5) :1304- 1312. PMCID: PMC3997530
Calvo MS, Mehrotra A, Beelman RB, Nadkarni G, Wang L, Cai W, et al Retrospective study in adults with metabolic syndrome: Diabetic risk factor response to daily consumption of Agaricus bisporus (white button mushrooms). Plants Foods Hum Nutr.2016; 71(3): 245-251.https://doi.org/ 10.1007/s11130-016-0552-7
Sharma VP, Sudheer K, Yogesh G, Manjit S ,Shwet K. Status of mushroom production in India. Mushroom Res. 2017; 26 (2) : 111-120.
Chechan RA. Optimal Conditions for Production of the Mother Culture for Cultivated Mushrooms Agaricus bisporus (White Iraqi Strain). Indian J Ecol. 2020; 47 (12): 225-230.
Avine FA, Bhassu S, Sabaratnam V. A simple and low-cost technique of DNA extraction from edible mushrooms examined by molecular phylogenetics. Res Crops. 2013; 14 (3) : 897-901.
Sambrook J, Russell DW. Molecular cloning: A laboratory manual. 3rd Ed. Cold Spring Harbor (NY): Cold Spring Harbor Laboratory Press.2001; 132-150.
Albaldawi A M, Chechan RA. Antioxidant Activity of phenolic extract from black tea. ANJS. 2010; 13(3): 99-103. http://doi.org/10.22401/JNUS.13.3.17.
Cho SM, Jang KY, Park HJ , Park JS. Analysis of the Chemical Constituents of Agaricu s brasiliensis. Mycobiology. 2008; 36(1): 50-54.http://doi.org/10.4489/ MYCO 2008.36.1.050
Xu Y, Qian SY . Anti-cancer Activities of ω-6 Polyunsaturated Fatty Acids. Biomed J. 2014; 37(3): 112–119. http:// doi.org/10.4103/2319-4170.131378
Díaz-Martínez YC, Guillén GK, Sánchez JE. Growth-promoting thermophilic microorganisms in self-heating pasteurized substrate improve Agaricusbisporus mycelial growth. Scientra fungorum. 2019; 49: 1261-1272. https://doi.org/10.33885/sf.2019.49.1261
Hsu L, Uen Y, Chen Y, Liang H, Kuo P. Tricetin a dietary flavonoid, inhibits proliferation of human breast adenocarcinoma MCF-7 cells by blocking cell cycle progression and inducing apoptosis. J Agric Food Chem. 2009; 57( 18): 8688–8695.http://doi.org/ 10.1021/jf901053x
Chen X, Cui L, Duan Xi, Ma B, Zhong D. Pharmacokinetics and metabolism of the flavonoid scutella in humans after a single oral administration . Drug Meta Dispos. 2006; 34(8): 1345–1352 .http://doi.org/ 10.1124/dmd.106.009779
Suzuki M , Fumiya N, Emi T, Maho N, Fumi W, Momoka T, et al. 4',6-Dimethoxyisoflavone-7-O-β-D glucopyranoside (wistin) is a peroxisome proliferator-activated receptor α (PPARα) agonist in mouse hepatocytes. Mol Cell Biochem 2018; 46(1-2): 35-41.https://doi.org/ 10.11 11/asj.12552
Zheng W, Wang B, Li X, Wang T , Zou H, Gu J. Zearalenone Promotes Cell Proliferation or CausesCell Death?. Toxins(Basel) . 2018; 10 (184): 1- 17. https://doi. org/10.3390/ toxins10050184
Ono M, Bishop DC, Oliver DL. Identified GABAergic and glutamatergic neurons in the mouse inferior colliculus share similar response properties. J Neurosci. .2017; 37: 8952–8964.http://doi:10.1523/ JNEUROSCI.0745-17.2017
Hamid AH , Ahmed KA., Fakhir RH, Rukaibaa AC .The Impact Of Edible mushroom Sspecies on lipid profiles and Blood picture of male balb- C mice. J Hunan Univ Nat Sci. 2021; 48(9): 338-347.
https://johuns.net/index.php/publishing/117.pdf
Huang Sh, Ning M, Zhiming L, Li G , Lisha D, Bin L, et al. Neuroprotective Effects of TaraxacumofficinaleWigg.Extract on Glutamate-Induced Oxidative Stress in HT22 Cells via HO-1/Nrf2 Pathways. Nutrients. 2018; 10(7): 926-938. https://doi.org/10.3390/nu10070926
Endrini S, Asmah R, Patimah I, Taufiq-Yap YH. Cytotoxic effect of γ-sitosterol from Kejibeling (Strobilanthescrispus) and its mechanism of action towards c-myc gene expression and apoptotic pathway. Med J Indones. 2014; 23(4): 203-208.https://doi.org/10.13181/mji.v23i4.1085
Lin PY, Chang CH, Chong MF, Chen H, Su KP .Polyunsaturated fatty cids in perinatal depression: a systematic review and meta-analysis. Biol Psychiatry. 2017; 82(8): 560-569.https://doi.org/%2010.1016/j%20.biopsych.2017.02.1182
Rizvi S, Syed TR, Faizal A, Absur A, Shania A. The Role of Vitamin E in Human Health and Some Diseases. Sultan Qaboos Univ Med J. 2014 ; 14(2): e157–e165.
Price A, Naomi EA, Paul NA, Francesca LC, Mazda J, Sabina R, et al. Plasma phytanic acid concentration and risk of prostate cancer: results from the European Prospective Investigation into Cancer and Nutrition. Am J Clin Nutr. 2010; 91: 1769–1776. https://doi.org/10.3945/ajcn.2009.28831.
Campia I, Lussiana C, Pescarmona G, Ghigo D, Bosia A, Riganti C..Geranylgeraniol prevents the cytotoxic effects of mevastatin in THP-1 cells, without decreasing the beneficial effects on cholesterol synthesis. Br J Pharmacol. 2009; 158: 1777–1786. https://doi.org/10.1111/j.1476-5381.2009.00465.x
Ning L, Hui M, Zhuyun J, Lu C , Li L , Qianfeng C , et al. Curcumol Suppresses Breast Cancer Cell Metastasis by Inhibiting MMP-9 Via JNK1/2 and Akt-Dependent NF-κB Signaling Pathways. Integr Cancer Ther. 2016; 15(2): 216-25.https://doi.org/ 10.1177/1534735416642865
Panda S, Jafri M, Kar A, Meheta BK.Thyroid inhibitory, antiperoxidative and hypoglycemic effects of stigmasterol isolated from Butea mono sperma. Fitoterapia. 2009; 80 (2): 123–126 . https://doi.org/10.1016/j.fitote.2008.12.002.
Zhang J, Zhao M , Lan Z , Guoyin Z , Liqin W , Mengshi J , et al. Purification and antioxidant activities of intracellular zinc polysaccharides from Pleurotus cornucopiae SS-03. Carbohydr Polym. 2014; 111: 947–954.https://doi.org/10.1016/j.carbpol.2014.04.074
Cheung PCK. The nutritional and health benefits of mushrooms. British Nutrition Foundation. British Nutrition Foundation. 2010; 35: 292– 299. https://doi.org/10.1111/j.1467-3010.2010.01859.x
Maseko T, Kate H, Frank RD, Ken N. Selenium-enriched Agaricus bisporus increases expression and activity of glutathione peroxidase-1 and expression of glutathione peroxidase-2 in rat colon. Food Chem. 2014; 146: 327–333.https://doi.org/10.1016/j.foodchem.2013.09.074
Watford M. Glutamine and glutamate: Nonessential or essential amino acids?.Reviewarticals. Anim Nutr. 2015; 1(3): 119-122.https://doi.org/10.1016/j.aninu.2015.08.008
Roshanzamir F, Seyyed MS. The putative effects of D-Aspartic acid on blood testosterone levels: A systematic review. Int J Reprod Bio Med . 2017; 15( 1): 1-10.https://doi.org/ 10.29252/ijrm.15.1.1
Said M I, Effendi A ,Sitti NS, Jamila T , Abdel Razzaq AT, AbdelRahman MAT. The Effect of white rot fungus (Ganoderma sp) as decomposers on composting using combination of cattle feces and water hyacinth (Eichhornia crassipes) as organic matter. Baghdad Sci J. 2022. 19(4): 775-786 . https://dx.doi.org /10.21123/bsj.202219.4.0775.
Abdullah DHM. Using Pomegranate Peel Extract to Change the Adverse effect of ethephon by enhancing its antioxidant, anti-inflammatory, and anti-apoptotic effects in rats. Baghdad Sci J. 2022. 19(5): 1045-1057. .https:/doi.org/10.21123/bsj.2022.6144.