دراسة تأثير الكركمين) قياسي و مكمل غذائي) والزنك على تراكيز الكلوكوز، الانسولين، مقاومة الانسولين وهرمون مضاد لمولر في نموذج الجرذان المصابة بتكيس المبايض المستحدث
محتوى المقالة الرئيسي
الملخص
الغرض من الدراسة الحالية للتحقق من تأثير الكركمين بشكليه (مكمل غذائي، قياسي) ، والزنك ، ثم استخدامهما معًا لإظهار تأثيرهما على مستويات الجلوكوز، الأنسولين، مقاومة الانسولين، والهرمون المضاد للمولر (AMH) في نموذج إناث الفئران المصابة بمتلازمة تكيس المبايض الناتجة عن تجريع الجرذان 1 ملغ / كجم / يوم من اليتروزول لمدة 21 يومًا تليها فترة علاج مدتها 14 يومًا من علاجات مختلفه شملت الزنك 30 مجم / كجم ، والكركمين القياسي 200 مجم / كجم ، ومكمل الكركمين 200 مجم / كجم ، (الكركمين القياسي مع الزنك) ، (مكمل الكركمين مع الزنك) و الميتفورمين كعلاج معتمد. بعد العلاج ، تم قتل جميع إناث الجرذان ، وجمعت عينات الدم من الوريد الأجوف السفلي لكل الجرذان للقياسات البيوكيميائية. تم قياس تراكيز الأنسولين و AMH باستخدام تقنية الاليزا ELISA. تم تحديد مستوى الجلوكوز باستخدام طريقة الطيفيه بينما تم قياس مقاومة الأنسولين حسابيا. أظهرت النتائج أن إعطاء الليتروزول كمثبط للأروماتاز أدى إلى ارتفاع كبير في الهرمون المضاد للمولر (AMH) ، الجلوكوز، الأنسولين، ومقاومة الأنسولين في النموذج المصمم لمتلازمة تكيس المبايض. اظهر الكركمين (القياسي و المكمل الغذائي) والزنك انخفاضًا معنويًا في مستويات الأنسولين في جميع مجموعات العلاج ، بينما كان التأثير أكثر وضوحًا عند تناول الزنك مع مكمل الكركمين.كما أظهرت النتائج أيضًا انخفاضًا معنويًا في الجلوكوز ومقاومة الانسولين، مما يدل على قدرة مكمل الكركمين والزنك على استعادة الجلوكوز و مقاومة الانسولين إلى مستواهما الطبيعي في مجموعة التحكم الصحية. انخفض هرمون مضاد مولر( AMH) بشكل معنوي لجميع المجموعات التي تناولت الكركمين بكلا الشكلين و الكركمين والزنك معًا ، بينما كان الانخفاض معنويًا للغاية في مكملات الكركمين والزنك.
Received 30/11/2022,
Revised 24/02/2023,
Accepted 26/02/2023,
Published Online First 20/07/2023
تفاصيل المقالة
هذا العمل مرخص بموجب Creative Commons Attribution 4.0 International License.
كيفية الاقتباس
المراجع
Lentscher JA, Decherney AH. Clinical Presentation and Diagnosis of Polycystic Ovarian Syndrome. Clin Obstet Gynecol. 2021 Mar 1; 64(1): 3-11. https://doi.org/10.1097/GRF.0000000000000563 . PMID: 32701517.
Aziz KMA Insulin Resistance, Obesity and Polycystic Ovarian Syndrome in Diabetic Patients. Diabetes Obes Int J. 2018 Jan; (3) ISSN: 2574-7770 Issue 1. https://doi.org/10.23880/doij-16000173 .
Fazel Torshizi F, Chamani M, Khodaei HR, Sadeghi AA, Hejazi SH, Majidzadeh Heravi R. Therapeutic effects of organic zinc on reproductive hormones, insulin resistance, and mTOR expression, as a novel component, in a rat model of polycystic ovary syndrome. Iran J Basic Med Sci. 2020 Jan; 23(1): 36-45. https://doi.org/10.22038/IJBMS.2019.36004.8586 .
Azziz R. Polycystic Ovary Syndrome. Obstet Gynecol. 2018 Aug; 132(2): 321-336. 1 https://doi.org/0.1097/AOG.0000000000002698 .
Azin F, Khazali H. Phytotherapy of polycystic ovary syndrome: A review. Int J Reprod Biomed. 2022 Feb 18; 20(1): 13-20. https://doi.org/10.18502/ijrm.v20i1.10404 .
Rocha AL, Oliveira FR, Azevedo RC, Silva VA, Peres TM, Candido AL, et al. Recent advances in the understanding and management of polycystic ovary syndrome. F1000Res. 2019 Apr 26; 8: F1000 Faculty Rev-565. https://doi.org/10.12688/f1000research.15318.1 .
Shaaban Z, Khoradmehr A, Jafar zadeh Shirazi MR, Tamadon A. Pathophysiological mechanisms of gonadotropins- and steroid hormones-related genes in etiology of polycystic ovary syndrome. Iran J Basic Med Sci. 2019 Jan; 22(1): 3-16. https://doi.org/10.22038/ijbms.2018.31776.7646 .
Al-Fartosy AJM, Awad NA, Mohammed AH. Intelectin-1 and Endocrinological Parameters in Women with Polycystic Ovary Syndrome: Effect of Insulin Resistance. Ewha Med J. 2020; 43(1): 111.https://doi.org/10.12771/emj.2020.43.1.1
Mvondo MA, MzemdemT soplfack FI, Awounfack CF, Njamen D. The leaf aqueous extract of Myrianthusarboreus P. Beauv. (Cecropiaceae) improved letrozole-induced polycystic ovarian syndrome associated conditions and infertility in female wistar rats. BMC Complement Med Ther. 2020 Sep; 20(1): 275. https://doi.org/10.1186/s12906-020-03070-8
Azin F, Khazali H. Neuropeptide galanin and its effects on metabolic and reproductive disturbances in female rats with estradiol valerate (EV) - Induced polycystic ovary syndrome (PCOS). Neuropeptides. 2020 Apr; 80: 102026. https://doi.org/10.1016/j.npep.2020.102026 . Epub 2020 Feb 4.
Mohsen S G. Role of metformin, spironolactone treatment, and their combination in estradiol valerate induced polycystic ovarian female rats. The College of Veterinary Medicine - University of Basra. Ph.D. thesis. 2014.
Jakubczyk K, Drużga A, Katarzyna J, Skonieczna-Żydecka K. Antioxidant Potential of Curcumin-A Meta-Analysis of Randomized Clinical Trials. Antioxidants (Basel). 2020 Nov; 9(11): 1092. DOI: https://doi.org/10.3390/antiox9111092 . PMID: 33172016; PMCID: PMC7694612.
Her C, Venier-Julienne MC, Roger E Improvement of Curcumin Bioavailability for Medical Applications. Med Aromat Plants. Los Angeles. 2018; 7: 326. https://doi.org/10.4172/2167-0412.1000326d
Ng Q X, Soh A Y S, Loke W, Venkatanarayanan N, Lim D Y, Yeo W x-S. A Meta-Analysis of the Clinical Use of Curcumin for Irritable Bowel Syndrome (IBS). J Clin Med. 2018; 7: 298. https://doi.org/10.3390/jcm7100298
Fadus MC, Lau C, Bikhchandani J, Lynch HT. Curcumin: An age-old anti-inflammatory and anti-neoplastic agent. J Tradit Complement Med. 2016 Sep; 7(3): 339-346. https://doi.org/10.1016/j.jtcme.2016.08.002 .
Guerrero-Romero F, Simental -Mendía LE, Martínez-Aguilar G, Sánchez-Meraz MA, Gamboa-Gómez CI. Hypoglycemic and antioxidant effects of five commercial turmeric (Curcuma longa) supplements. J Food Biochem. 2020 Sep; 44(9): e13389. https://doi.org/10.1111/jfbc.13389 . Epub 2020 Jul 21.
Fan C, Song Q, Wang P, Li Y, Yang M, Yu S Y. Neuroprotective Effects of Curcumin on IL-1beta-Induced Neuronal Apoptosis and Depression-Like Behaviors Caused by Chronic Stress in Rats. Front Cell Neurosci. 2018; 12: 516. https://doi.org/10.3389/fncel.2018.00516 .
Mohmmed A H, Awad N A, AL-Fartosy A J. Study of trace elements selenium, copper, zinc and manganese level in polycystic ovary syndrome (PCOS) Int J Res Appli Sci. Biotechnol. 2019; 6 https://doi.org/10.31033/ijrasb.6.6.4 .
Musadak A and Zearah SA. Effects of Serotonin, Melatonin, and Zinc Levels on Prolactin Hormone in Hyperprolactinemic Patients in Basrah Governorate, Iraq. Trop J Nat Prod Res. 2022; 6(5): 740-744 http://www.doi.org/10.26538/tjnpr/v6i5.14
Pokorska-Niewiada K., Brodowska A., Szczuko M. The Content of Minerals in the PCOS Group and the Correlation with the Parameters of Metabolism. Nutrients. 2021; 7: 2214. https://doi.org/10.3390/nu13072214
Reddy PS, Begum N, Mutha S, Bakshi V. Beneficial effect of curcumin in letrozole induced polycystic ovary syndrome. Asian Pac J Reprod. 2016 April; 5: 116–122. https://doi.org/10.1016/j.apjr.2016.01.006
Shen HR, Xu X, Li XL. Berberine exerts a protective effect on rats with polycystic ovary syndrome by inhibiting the inflammatory response and cell apoptosis. Reprod Biol Endocrinol. 2021; 19: 3. https://doi.org/10.1186/s12958-020-00684-y .
Purwar A, Nagpure S, Insulin Resistance in Polycystic Ovarian Syndrome. Cureus. 2022.Oct; 14(10): e30351. https://doi.org/10.7759/cureus.30351
Shukrya H. Alwan, Muna H. Al-Saeed. Biosynthesized Silver Nanoparticles (using CinnamomumZeylanicum Bark Extract) Improve the Fertility Status of Rats with Polycystic Ovarian Syndrome. Biocatal Agric Biotechno. 2021; 38: 102217. DOI: 10.1016/j.bcab.2021.102217
Al-Moziel, M S G, Alkalby J M A, Sawad A A. Relationship Between Insulin Resistance and Serum Concentrations of Resistin and Insulin-Like Growth Factor-I (Igf-I) Associated with Induced Polycystic Ovary Syndrome in Female Rats. Bas J Vet Res. 2013; 12(2): 164-171. https://www.iasj.net/iasj/article/83649
Guler I, Himmetoglu O, Turp A, Erdem A, Erdem M, Onan MA, et al. Zinc and homocysteine levels in polycystic ovarian syndrome patients with insulin resistance. Biol Trace Elem Res. 2014 Jun; 158(3): 297-304. https://doi.org/10.1007/s12011-014-9941-7 . Epub 2014 Mar 26. PMID: 24664271.
Beletate V, El Dib RP, Atallah AN. Zinc supplementation for the prevention of type 2 diabetes mellitus. Cochrane Database Syst Rev. 2007Jan; 1: CD005525. https://doi.org/10.1002/14651858.CD005525.pub2
Karateke A, Dokuyucu R, Dogan H, Ozgur T, Tas ZA, Tutuk O, et al. Investigation of Therapeutic Effects of Erdosteine on Polycystic Ovary Syndrome in a Rat Model. Med Princ Pract. 2018; 27(6): 515-522. https://doi.org/10.1159/000494300 .
Fouad Z HB. Effect of Ethanolic Extract of Triganella Foenum greacum L. on Polycystic Ovary Syndrome Induced by Letrozole in Female Rats. The College of Veterinary Medicine - University of Basra. Ph.D. thesis, 2018.
Guan Y, Wang D, Bu H, Zhao T, Wang H. The Effect of Metformin on Polycystic Ovary Syndrome in Overweight Women: A Systematic Review and Meta-Analysis of Randomized Controlled Trials. Int J Endocrinol. 2020 Sep; 2020: 5150684. https://doi.org/10.1155/2020/5150684
Xing C, Zhang J, Zhao H, He B. Effect of Sex Hormone-Binding Globulin on Polycystic Ovary Syndrome: Mechanisms, Manifestations,Genetics, and Treatment. Int J Womens Health. 2022; 14: 91-105
https://doi.org/10.2147/IJWH.S344542
Prapas N, Karkanaki A, Prapas I, Kalogiannidis I, Katsikis I, Panidis D. Genetics of polycystic ovary syndrome. Hippokratia. 2009 Oct; 13(4): 216-23. PMID: 20011085; PMCID: PMC2776334.
Ding H, Zhang J, Zhang F, Zhang S, Chen X, Liang W, et al. Resistance to the Insulin and Elevated Level of Androgen: A Major Cause of Polycystic Ovary Syndrome. Front Endocrinol (Lausanne). 2021 Oct; 20 (12). https://doi.org/10.3389/fendo.2021.741764 . PMID: 34745009.
Diamanti-Kandarakis E, Dunaif A. Insulin resistance and the polycystic ovary syndrome revisited: an update on mechanisms and implications. Endocr Rev. 2012 Dec; 33(6): 981-1030. https://doi.org/10.1210/er.2011-1034 .
Asadi S, Gholami M S, Siassi F, Qorbani M, Khamoshian K, Sotoudeh G. Nano curcumin supplementation reduced the severity of diabetic sensorimotor polyneuropathy in patients with type 2 diabetes mellitus: A randomized double-blind placebo-controlled clinical trial. Complement Ther Med. 2019; 43: 253–260. https://doi.org/10.1016/j.ctim.2019.02.014 .
Basham S A, Waldman H S, Krings B M, Lamberth J, Smith J W, McAllister M J. Effect of Curcumin Supplementation on Exercise-Induced Oxidative Stress, Inflammation, Muscle Damage, and Muscle Soreness. J Diet Suppl. 2019; 17: 401–414. https://doi.org/10.1080/19390211.2019.1604604 .
de Oliveira TV, Stein R, de Andrade DF, Beck RCR. Preclinical studies of the antitumor effect of curcumin-loaded polymeric nanocapsules: A systematic review and meta-analysis. Phytother Res. 2022; 36(8): 3202-3214. https://doi.org/10.1002/ptr.7538
Dai QD, Zhou D, Xu LP, Song XW. Curcumin alleviates rheumatoid arthritis-induced inflammation and synovial hyperplasia by targeting mTOR pathway in rats. Drug Des Devel Ther. 2018; 12: 4095-4105. https://doi.org/10.2147/DDDT.S175763
Roy A, Das S, Chatterjee I, Roy S, Chakraborty R. Anti-inflammatory Effects of Different Dietary Antioxidants. In: Ekiert, H M, Ramawat, K G, Arora J. (eds) Plant Antioxidants and Health. Reference Series in Phytochemistry. 2022: 1-25. Springer. Cham. https://doi.org/10.1007/978-3-030-45299-5_20-1
Alizadeh M, Kheirouri S. Curcumin reduces malondialdehyde and improves antioxidants in humans with diseased conditions: a comprehensive meta-analysis of randomized controlled trials. Biomedicine (Taipei). 2019 Dec; 9(4): 23. https://doi.org/10.1051/bmdcn/2019090423 . Epub 2019 Nov 14. PMID: 31724938; PMCID: PMC6855189.
Wang MX, Yin Q, Xu X. A Rat Model of Polycystic Ovary Syndrome with Insulin Resistance Induced by Letrozole Combined with High Fat Diet. Med Sci Monit. 2020; 26: e922136. https://doi.org/10.12659/MSM.922136
Norouzi S, Adulcikas J, Sohal SS, Myers S. Zinc stimulates glucose oxidation and glycemic control by modulating the insulin signaling pathway in human and mouse skeletal muscle cell lines. PLoS One. 2018 Jan 26; 13(1): e0191727. https://doi.org/10.1371/journal.pone.0191727 . PMID: 29373583.
Hussain AM, Ali AH, Mohammed HL. Correlation between Serum and Tissue Markers in Breast Cancer Iraqi Patients. Baghdad Sci J. 2022Jun.1 19(3): 0501. https://doi.org/10.21123/bsj.2022.19.3.0501
Aydogan Mathyk B, Cetin E, Yildiz BO. Use of anti-Müllerian hormone for understanding ovulatory dysfunction in polycystic ovarian syndrome. Curr Opin Endocrinol Diabetes Obes. 2022 Dec 1; 29(6): 528-534. https://doi.org/10.1097/MED.0000000000000772 . Epub 2022 Oct 11. PMID: 36218229.
Lomteva S V, Shkurat T P, Bugrimova E S, Zolotykh O S, lexandrova A A, Karantysh G V. Violation of the Hormonal Spectrum in Polycystic Ovaries in Combination with Insulin Resistance. What is the Trigger: Insulin Resistance or Polycystic Ovary Disease? Baghdad Sci J. 2022Oct.1; 19(5): 0990. http://dx.doi.org/10.21123/bsj.2022.6317
Morsy MA, El-Hussieny M, Zenhom NM, Nair AB, Venugopala KN, Refaie MMM. Fenofibrate ameliorates letrozole-induced polycystic ovary in rats via modulation of PPARα and TNFα/CD95 pathway. Eur Rev Med Pharmacol Sci. 2022 Oct; 26(20): 7359-7370. https://doi.org/10.26355/eurrev_202210_30005 .
Ran Y, Yi Q, Li C. The Relationship of Anti-Mullerian Hormone in Polycystic Ovary Syndrome Patients with Different Subgroups. Diabetes Metab Syndr Obes. 2021 Mar; 14: 1419-1424. https://doi.org/10.2147/DMSO.S299558
Tian, FJ Diaz, Zinc depletion causes multiple defects in ovarian function during the periovulatory period in rats, Endocrinology, 2012Feb; 153(2): 873-886, https://doi.org/10.1210/en.2011-1599
Foroozanfard F, Samimi M, Almadani KH, Sehat M. Effect of metformin on the anti-Müllerian hormone level in infertile women with polycystic ovarian syndrome. Electron Physician. 2017; 9(12): 5969-5973. Published 2017 Dec 25. https://doi.org/10.19082/5969
Heshmati J, Moini A, Sepidarkish M, Morvaridzadeh M, Salehi M, Palmowski A et al. Effects of curcumin supplementation on blood glucose, insulin resistance and androgens in patients with polycystic ovary syndrome: A randomized double-blind placebo-controlled clinical trial. Phytomedicine. 2021 Jan; 80: 153395. https://doi.org/10.1016/j.phymed.2020.153395. Epub 2020 Oct 22. PMID: 33137599.