الكشف عن بعض عوامل الضراوة بين المبيضات المعزولة من المرضى وانتشار جين candidalysin ECE1

محتوى المقالة الرئيسي

Safaa A. S. Al-Qaysi
Sokayna R. M. AL-Rubaie AL-Rubaie
https://orcid.org/0009-0000-0342-5459

الملخص

المبيضات البيضاء هي مسبب شائع لعدوى الجهاز التنفسي وداء المبيضات الفموي لدى الناس ، ومسببات أمراض الخميرة الانتهازية والأسباب الرئيسية للمرض والوفيات لدى الأشخاص الذين يعانون من نقص المناعة وتسبب التهابات سطحية على الأسطح المخاطية التي تصيب ملايين الأشخاص في جميع أنحاء العالم.  كان الهدف الرئيسي من هذه الدراسة هو التحقق من انتشار بعض عوامل الضراوة التي لها القدرة على تكوين الأغشية الحيوية (Biofilm)، والبروتينيز  (Proteinase)، وإنتاج الهيمولايسين( Hymolysine) بين  عزلات  C. albicans التي تشمل انتشار جين Candidalysin (ECE1).  تم جمع العينات خلال الفترة من ايار 2022 الى اب 2022  من 280 عينة (مسحة) لمرضى عراقيين  من مختلف الاعمار والاجناس غير مكررين  كانوا يعانون من داء المبيضات الفموي وامراض الجهاز التنفسي في مستشفيات بغداد (مستشفى مدينه الطب و مستشفى اليرموك التعليمي ومستشفى مدينه الامامين الطبية التعليمية).  تم زراعة العينات التي تم جمعها على وسط اكار  السابرويد  دكستروز (SDA) مضاف اليه كلورامفينيكول كمضاد للبكتيريا بتركيز 10 ميكجم / مل ، وتم حضن الاطباق الملقحة على وسط SDA عند 37 درجة مئوية لمدة تتراوح بين 24-48 ساعة.أظهرت نتائج الاستنبات أن 102 عينة كانت موجبه، منها58 (56.86%) من تجويف الفم و 44 (43.14%) من الجهاز التنفسي،  بينما 178 منها كانت سالبة , تم الكشف عن عزلات المبيضات باستخدام الطرق التقليدية والتي تشمل (النمو على وسط HiCrome Candida ، إنتاج الأنبوب الجرثومي Germ tube ، تكوين الابواغ الكلاميديه Clamydospore   وتم تأكيد نتئج التعريف باستخدام نضام VITEK-2  بينت النتائج ان من بين 102 عزلة C. albicans هي الاكثر شيوعا بين الانواع بنسبة 68.63 % ثم تلتها C. tropicalis  بنسبة %10.78  ثم C. kruzei %5.88  وC. kefyr %5.88 C. parapsilosis % 4.9  بينما كانت C. glabrata %  3.9  وتمثل النسبة الاقل بين الانواع . تم فحص حساسية عزلات المبيضات للأدوية المضادة للفطريات بطريقة انتشار القرص ، والتي أجريت على النحو الموصى به بواسطة(CLSI) M44-A -وثيقة  . أظهرت العزلات درجة عالية من الحساسية للأمفوتريسين-ب 93.14%    وكلوتريمازول 90.20% ونسياتين 85.92% ،تم التحقق من انتشار جينCandidalysin (ECE1)  بين 70 عزلة من C. albicans باستخدام تقنية تفاعل البلمرة المتسلسل  PCR  ، وأظهرت النتائج أن 41  58.57%  تحتوي على جين Ece1 في تجويف الفم والجهاز التنفسي. و 34 عزلة  48.57%  من C. albicans كانت منتجة قوية للغشاء الحيوي,  بينما 30 عزلة 42.86%  عزلة انتجت بروتيناز و 20 عزلة  28.57%  عزلة لديها القدرة على تحلل الدم.

تفاصيل المقالة

كيفية الاقتباس
1.
الكشف عن بعض عوامل الضراوة بين المبيضات المعزولة من المرضى وانتشار جين candidalysin ECE1. Baghdad Sci.J [انترنت]. 1 أبريل، 2024 [وثق 21 مايو، 2024];21(4):1173. موجود في: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/8727
القسم
article

كيفية الاقتباس

1.
الكشف عن بعض عوامل الضراوة بين المبيضات المعزولة من المرضى وانتشار جين candidalysin ECE1. Baghdad Sci.J [انترنت]. 1 أبريل، 2024 [وثق 21 مايو، 2024];21(4):1173. موجود في: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/8727

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