Compatibility between Pseudomonas fluorescens and Trichoderma harzianum in disease control of Fusarium tomato wilt under greenhouse condition

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I . A. Jediaa
A . A. Ali
B. A. Abbas
Sh . A. Musa
H.R. Hassan
L. J. Muhamed

Abstract

        This study was conducted to evaluate the efficacy of  6 isolates of Pseudomonas fluorescens and Trichoderma harzianum and there combination against Fusarium tomato wilt disease caused by Fusarium oxysporum F.sp. Lycopersisi under green house condition .The isolates of bacteria (B3) and Trichoderma (T1) were found to be highly effective in reducing the disease incidence to 13.3% , 21% respectively , compared to control treatment (40%).Furthermore, disease severity was reduced to 28 and 30%  respectively in comparison to control (90%) .Colonization of the roots (cfu /g fresh root weight )by the two isolates whether alon or together was extremely high . The combination treatment had a high ability in reducing disease incidenece and severity to 10,3 , 16% respectively compared to control (36.3% , 88 % respectively), and highly effective in increasing some growth parameters .

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1.
Compatibility between Pseudomonas fluorescens and Trichoderma harzianum in disease control of Fusarium tomato wilt under greenhouse condition . Baghdad Sci.J [Internet]. 2009 Mar. 1 [cited 2024 Oct. 19];6(1):1-9. Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/11882
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How to Cite

1.
Compatibility between Pseudomonas fluorescens and Trichoderma harzianum in disease control of Fusarium tomato wilt under greenhouse condition . Baghdad Sci.J [Internet]. 2009 Mar. 1 [cited 2024 Oct. 19];6(1):1-9. Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/11882

References

الدليمي, اسماعيل عباس واياد عبد الواحدالهيتي (2001). المكافحة الاحيائية لمسبب مرض سقوط البادرات aphanidermatum Pythium بالبكتريا fluorescens Pseudomonas تحت ظروف البيت الزجاجي .مجلة العلوم الزراعية .مجلد 32، العدد 6 , ص113- 120 .

جرجيس ,ميسر مجيد ,رقيب عاكف العاني واياد عبد الواحد الهيتي (1993).امراض النبات,دار الحكمة للطباعة والنشر ,العراق ,ص 569.

ALabouvette,C., Lemanceau , P.and Steinberg ,C.(1993).Recent advances in the biolgical control of Fusarium wilts .Pestic.sci.37: 365-373.

Elad, Y.,Chet, J.and Henis,Y. (1981).Aselective medium for improving quantitative isolation of Trichoderma spp. from soil . phytoparasitica 9:59-67.

Datnoff , L .E., Nemec,S. and Pernezny , K. (1994) .Biological control of Fusarium crown and root rot using beneficial fungi. In "proceedings of the florida Tomato Institute, PRO 105 "(C.S.varrina, Ed.),PP,55-64. Hort .Sci. Dept. Univ.Florida, Gainesville.

Datnoff , L.E., Nemec,S.and Pernezny, K. (1995). Biologcal control of Fusarium crown and root rot of tomato in florida using Trichoderma harzianum and Glomus intraradices .Bilogical Control 5:427_431 .

Decal ,S.P.and P.Melgarso (1997) . Infectivity of chlamydospore VS.microconidia of Fusarium oxysporum f.sp.lycopersici on tomato .J .Phytopathology ,145:231-233.

Duijff ,B .J.,Recorbet,G.,Bakker ,P.A.H.M.,Loper ,J.E., and lemanceau,P.(1999).Microbial antagonism at the root level is involved in the suppression of Fusarium oxysporum Fo 47 and Pseudomonas putida WCS 358.Phytopath.89; 1073-1079 .

Gamliel, A. and katan .J .(1993). Influence of seed and root exudates of fluorescent pseudomonads and fungi solarized soil .Phytopath. 82 :320-327 .

Hadar,Y.,I.chet and Henis .Y.(1979).Biological control of Rhizoctonia solani damping-off with wheat bran culture of Trichoderma harzianum Phytopath. 69;64-68.

Harman,G.E.(2000).Myths and dogmas of biocontrol changes in perceptions derived from research on Trichoderma harzianum T.22.PlantDisease,84 (4): 377-393.

Jarvis, W.R. (1988) Fusarium crown and root rot of tomatoes Phytoprotection 69: 49-64.

Jones,J.P., Woltz,S.E., and Scott, J.W.(1991). Fusarium crown rot of tomato , some factors affecting disease development .In : proceedings of the fiorida Tomato Institute ,SS-VEGOL Veg. Creps Special Series ,W.M. stall,ed.,PP.74-79.Veg.Crops Dept .,Univ .Florida,Gainesville .

King ,E.O.,Ward ,M.K.,and Raney ,D.E.(1954).Two simple media for the demonstration of Pyocyanin and fluoresin. J.lab.Clin. Med. 44:301-307 .

Lemanceau,P.(1989). Role of competition for carbon and iron in mechanisms of soil suppressiveness to Fusarium wilts. pages 386-396 In :vascular wilt disease of plants ,basic studies and control ,E.C. Tjamos and C.H. Beckman ,eds Springer Verlag ,Berlin .

Lemanceau, P., and ALabouvette ,C.(1993).Suppressione of Fusarium wilts by fluorescent pseudomonads : Mechanisms and application .Biocontrol Sci. Technol . 3: 219 – 234.

Liu, L .Kloepper, J.W.and Tuzun ,S.(1995).Induction of systemic resistance in cucumber against Fusarium wilt by plant growth- promoting rhizobacteria . Phytopatho.85: 695- 698 .

Mc Govern ,R.J., Datnoff , L.E.and Varina ,C.S., (1993a). Evaluation of seven tomato genotypes for resistance to Fusarium oxysporum f.sp. radicis-lycopersici .Phytopathol .83:1395 .

Mc Govern ,R.J.,Datnoff ,L.E.,Secker,I.,Vavrina,G.S., Capece ,J.C.and Noling ,J.W..(1993 b).New developments in the management of Fusarium crown and root rot of tomato in southwest Florida. PP.45-64.In: proceedings of the Florida Tomato Institute, PRO 105,C.S.Vavrina, ed.,Unive.of Florida , Horti. Sci. Dept ., Institute of food and agricultural sciences.

Nemec,S., Datnoff ,L.and Strand berg ,J.(1996).Efficacy of gents in planting mixes to colonize plant roots and control root diseases of vegetables and ciitrus .Crop Protection 15:735 -742.

Rowe, R.C. and Farley ,J.D. (1981). Strategies for controlling Fusarium crown and root rot in greenhouse tomatoes. Plant Disease 65;107-112 .

Sivan , A.Ucko , O. and Chet , I .(1987).Biological control of Fusarium crown rot of tomato by Trichoderma harzianum under field condition . Plant Disease 71: 587-592 .

Vanloon ,L . C . , Bakker ,P . A . H . M . and Peterse , C . M . J . (1998). Systemic resistance induced by rhizosphere bacteria . Annu . Rev . phytopathol . 36 : 453 -483 .

Weller ,D . M . ( 1988 ) . Biological control of Soil – borne plant pathogens in the rhizosphere with bacteria . Ann . Rev. Phytopathol. 26 : 379 – 407 .