Antibacterial Activity of Silver Nanoparticles Synthesized by Aqueous Extract of Carthamus oxycantha M.Bieb. Against Antibiotics Resistant Bacteria
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Abstract
Antibiotics resistant bacteria have become a global problem as a result of the unprogrammed use of antibiotics, resulting in bacterial strains resistant to many antibiotics, or to all available antibiotics. Plants are a good source of primary and secondary metabolites that have a major role in reducing silver nitrate to silver nanoparticles (AgNPs). The production of these nanoparticles were carried out by using aqueous extract of Carthamus oxycantha M.Bieb. This can be verified by color change of the reaction solution from yellow to dark brown because of the excitation of the surface plasmon resonance. AgNPs were characterized by UV-Vis spectroscopy, where they recorded the peak at 420 nm. Fourier Transformation-infrared (FTIR) was conducted to identify the effective plant group that contributes to the formation of AgNPs and it was found that proteins and phenols have the major role in the formation of those nanoparticles. Shapes and sizes of the synthesized AgNPs were characterized by Scanning Electron Microscope (SEM) with a range of 50-80nm in size and spherical in shapes. Antibacterial activity of AgNPs were tested against Multi-Drug Resistant bacteria (MDR), Extremely antibiotics Resistant (XDR), and Pan drug-resistant (PAN) bacteria, was done in concentrations ranging from 1000-63 µg/ml. The results showed that there were significant variations between the concentrations, the tested bacteria also showed significant differences in its sensitivity to AgNPs. The results recorded a proportional relation between the type of bacterial resistance to antibiotics and it's resistant to AgNPs, therefore the most resistant bacteria to AgNPs in this study Enterobacter cloacae EN2 was resistant to all antibiotics (PAN), while Escherichia coli E11 recorded was the most sensitive bacteria to AgNPs and its resistant only to 3 antibiotics.
unprogrammed use of antibiotics, resulting in bacterial strains resistant to many
antibiotics, or to all available antibiotics. Plants are a good source of primary and
secondary metabolites that have a major role in reducing silver nitrate to silver
nanoparticles (AgNPs). The production of these nanoparticles were carried out by using
aqueous extract of Carthamus oxycantha M.Bieb. This can be verified by color changed
of the reaction solution from yellow to dark brown because of the excitation of the
surface plasmon resonance. AgNPs were characterized by UV-Vis spectroscopy, where
recorded peak at 425 nm. Fourier Transformation-infrared (FTIR) was conducted to
identify the effective plant group that contributes to the formation of AgNPS and it was
found that proteins and phenols have the major role in the formation of those
nanoparticles. Shapes and sizes of synthesized AgNPs were characterized by Scanning
Electron Microscope (SEM) with a range of 50-80nm in size and spherical in shapes.
Antibacterial activity of AgNPs were tested against Multi-Drug Resistant bacteria
(MDR), Extremely antibiotics Resistant (XDR), and Pandrug-resistant (PAN) bacteria,
was done in concentrations ranging from 1000-63 µg/ml. The result showed that the
concentrations from 1000-125 µg/ml inhibited all tested bacterial strains except the S1
strain
Received 11/6/2020, Accepted 11/2/2021, Published Online First 20/11/2021
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References
Baptista PV, McCusker M P, Carvalho A, Ferreira D A, Mohan N M, Martins M, et al. Nano-Strategies to Fight Multidrug Resistant Bacteria—“A Battle of the Titans.” Front Microbiol . 2018 July; 9: 1-26.
Vivas R, Barbosa AA, Dolabela SS, Jain S. Multidrug-resistant bacteria and alternative methods to control them: an overview. Microb Drug Resist. 2019 Jul 1;25(6):890-908.
Zhang Y, Chen XL, Huang AW, Liu SL, Liu WJ, Zhang N, et al. Mortality attributable to carbapenem-resistant Pseudomonas aeruginosa bacteremia: a meta-analysis of cohort studies. Emerg Microbes Infect. 2016 Jan 1;5(1):1-6.
-Yadi M, Mostafavi E, Saleh B, Davaran S, Aliyeva I, Khalilov R, et al. Current developments in green synthesis of metallic nanoparticles using plant extracts: a review. Artif Cells Nanomed Biotechnol . 2018 Nov 12;46(sup3):S336-43.
Yang, X. A study on antimicrobial effects of nanosilver for drinking water disinfection. Springer Theses.2017; 1: 13-36.
Nadkarni, K. M. Indian Materia Medica. Popular prakashan , Bombay, Vol. 1, 1976,pp. 469-470.
Chopra RN, Chopra IC, Handa KL , Kapur LD. Chopra’s Indigenous Drugs of India. 1982,2nd ed, Academic Publishers, Calcutta, New Delhi, pp.505-506.
Anjani K. Genetic variability and character association in wild safflower (Carthamus oxyacantha). Indian J. Agric. Sci. 2005;75(8):516-8.
Dhanani T, Shah S, Gajbhiye NA, Kumar S. Effect of extraction methods on yield, phytochemical constituents and antioxidant activity of Withania somnifera. Arab. J. Chem. 2017 Feb 1;10:S1193-9.
Shankar SS, Rai A, Ahmad A, Sastry M. Rapid synthesis of Au, Ag, and bimetallic Au core–Ag shell nanoparticles using Neem (Azadirachta indica) leaf broth. J Colloid Interface Sci. 2004 Jul 15;275(2):496-502.
Elbeshehy EK, Elazzazy AM, Aggelis G. Silver nanoparticles synthesis mediated by new isolates of Bacillus spp., nanoparticle characterization and their activity against Bean Yellow Mosaic Virus and human pathogens. Front Microbiol . 2015 May 13;6:453.
Islam M, Yesmin R, Ali H, Ayshasiddeka , Karmakar PC, Habib R, et al. Antineoplastic Properties of Phyto-synthesized Silver Nanoparticles from Hibiscus Sabdariffa Linn. Bark Extract. CAJMS. 2018; 4:281-292.
Yu C, Tang J, Liu X, Ren X, Zhen M, Wang L. Green biosynthesis of silver nanoparticles using Eriobotrya japonica (Thunb.) leaf extract for reductive catalysis. Materials. 2019 Jan;12(1):189.
Lalitha A, Subbaiya R, Ponmurugan P. Green synthesis of silver nanoparticles from leaf extract Azhadirachta indica and to study its anti-bacterial and antioxidant property. Int J Curr Microbiol App Sci. 2013; 2: 228–35.
Anil Kumar V, Ammani K, Jobina R, Parasuraman P, Siddhardha B. Larvicidal activity of green synthesized silver nanoparticles using Excoecaria agallocha L. (Euphorbiaceae) leaf extract against Aedes aegypti . IET Nanobiotechnol. 2016; 10(6): 382–388.
Aboutorabi S N, Nasiriboroumand M, Mohammadi P, Sheibani H, Barani H. Biosynthesis of Silver Nanoparticles Using Safflower Flower: Structural Characterization, and Its Antibacterial Activity on Applied Wool Fabric. JIOPM. 2016; 28 (6): 2525-2532.
Mittal A K, Chisti Y, Banerjee U C. Synthesis of metallic nanoparticles using plant extracts. Biotechnol Adv. 2013; 31(2): 346–356.
Masum M, Siddiqa M M, Ali K A, Zhang Y, Abdallah Y, Ibrahim E, et al. Biogenic Synthesis of Silver Nanoparticles Using Phyllanthus emblica Fruit Extract and Its Inhibitory Action Against the Pathogen Acidovorax oryzae Strain RS-2 of Rice Bacterial Brown Stripe. Front Microbiol. 2019 Apr 26; 10:820.
Ayad ZM, Ibrahim OMS, Omar LW. Biosynthesis and characterization of silver nanoparticles by silybum marianum (silymarin) fruit extract. Adv. Anim. Vet. Sci. 2019;7: 122-130.
Kumar D, Chadda S, Sharma J, Surain P. Syntheses, Spectral Characterization, and Antimicrobial Studies on the Coordination Compounds of Metal Ions with Schiff Base Containing Both Aliphatic and Aromatic Hydrazide Moieties. Bioinorg Chem Appl. 2013 Oct 3;2013: 981764.
Shah A T, Din M I, Bashir S, Qadir M A, Rashid F. Green Synthesis and Characterization of Silver Nanoparticles UsingFerocactus echidneExtract as a Reducing Agent. Anal. Lett. 2015 May 3;48(7):1180-9.
Kumar B, Jalodia K, Kumar P, Gautam HK. Recent advances in nanoparticle-mediated drug delivery. J. Drug Deliv. Sci. Technol. 2017; 41: 260–268.
Kumar PV, Pammi SV, Kollu P, Satyanarayana KV, Shameem U. Green synthesis and characterization of silver nanoparticles using Boerhaavia diffusa plant extract and their anti bacterial activity. Industrial Crops and Products. 2014 Jan 1;52:562-6.
Parameshwaran R, Kalaiselvam S, Jayavel R. Green synthesis of silver nanoparticles using Beta vulgaris: Role of process conditions on size distribution and surface structure. Materials Chemistry and Physics. 2013; 140(1): 135–147.
Finley P J, Norton R, Austin C, Mitchell A, Zank S, Durham P. Unprecedented silver resistance in clinically isolated Enterobacteriaceae: major implications for burn and wound management. Antimicrob. Agents Chemother. 2015; 59: 4734–4741.
Pelgrift RY, Friedman AJ. Nanotechnology as a therapeutic tool to combat microbial resistance. Adv. Drug Deliv. Rev. 2013 Nov 30;65(13-14):1803-15.
Graves Jr JL, Tajkarimi M, Cunningham Q, Campbell A, Nonga H, Harrison SH, Barrick JE. Rapid evolution of silver nanoparticle resistance in Escherichia coli. Front. Genet. 2015 Feb 17;6:42.
Hemeg H A. Nanomaterials for alternative antibacterial therapy. Int. J. Nanomed.2017; 12, 8211–8225.
Panáček A, Kvítek L, Smékalová M, Večeřová R, Kolář M, Röderová M, et al. Bacterial resistance to silver nanoparticles and how to overcome it. Nat. Nanotechnol.2017; 13(1): 65–71.
Slavin YN, Asnis J, Häfeli UO, Bach H. Metal nanoparticles: understanding the mechanisms behind antibacterial activity. J Nanobiotechnology. 2017 Oct 3;15(1):65.