Bioethanol Production by Candida tropicalis Isolated from Sheep Dung
DOI:
https://doi.org/10.21123/bsj.2023.6743Keywords:
Bioethanol, Candida tropicalis, Cellulase, Dung, Optimum, tolerateAbstract
Microorganisms have an active role in biotechnology for example yeasts, especially in some genus like Saccharomyces, Pichia, and Candida. C.tropicalis one of the most important species of Candida and despite it is one of the causative agents of candidiasis but it has a major role in the production of many chemical compounds. C.tropicalis in the previous study was isolated from sheep dung and morphologically and molecularly classified the result of sequencing was elucidate 100% similarity between the studied isolate and other isolates inserted in DNA Data Bank of Japan DDBJ, physiologically this isolate tolerated 6% ethanol concentration in broth media with the ability to the production of 4% ethanol under optimum conditions including (YEPDB) medium, incubation temperature 30◦C, PH 6 and dextrose as a carbon source which give more productivity as a carbon source and optimum incubation period was three days . This isolate gives a positive result for the cellulase enzyme-producing test and an exciting feature to remove effluent because it completely adsorbed the Congo red dye from dye solation in a liquid growth medium.
Received 10/11/2021
Accepted 26/7/2022
Published Online First 20/1/2023
References
Nigam PS, Singh A. Production of liquid biofuels from renewable resources. Prog Energy Combust Sci. 2011; 37: 52-68.
Hajar S, Azhar M, Abdulla R, Jambo S, Marbawi H, Gansau J, et al . Yeasts in sustainable bioethanol production, A review. Bioche Biophy Rep. 2017; 10: 52-61.
.Msi Z, Nurcholis M. Molecular Identification and Potential Ethanol Production of Long-term Thermo-Tolerant Yeast Candida Tropicalis. IOP Conf. Ser: Earth Environ. Sci. 2019; 23 9: 012004. https://doi.org/10.1088/1755-1315/239/1/012004
Dien, BS, Cotta MA, Jeffries TW. Bacteria engineered for fuel ethanol production: current status. Appl. Microbiol. Biotechnol. 2003; 63: 258-266.
Zheng J, Negi A, Khomlaem C, Kim BS. Comparison of Bioethanol Production by Candida molischiana and Saccharomyces cerevisiae from Glucose, Cellobiose, and Cellulose. J Microbiol Biotechnol. 2019;29(6):905-912 https://doi.org/10.4014/1904.04014.
Jafar FN. Candidiasis Types, Causative Agents, and Treatment Methods. Sci J Medi Res. 2021; 5(19): 90-93.
Shari M, Sohail M. Application of Candida tropicalis MK-160fot the production of xylanase and ethanol. J King Saud Uni Sci. 2019; 31( 4):1189-1194.
Wiratno EN , Rupilu NS. Isolasi, Identifikasi Dan Produksi Etanol Khamir Indigenous Nira Siwalan (Borassus flabellifer L.) Dari Tuban, Jawa Timur. Indones J Biotechnol 2018; 6 ( 1 ).
Latifa J, Sendide K, Ettayebi K, Errachidi F, Alami O, Tahri-Jouti M, et al . Physiological deference during ethanol fermentation between calcium alginate-immobilized Candida tropicalis and Saccharomyces cerevisiae. FEMS Microbiol. Lett . 2001; 204: 375-379.
Makhuvelea R, Ncubea I, Lukas E, Rensburga J ,Coenrad D, Grangeb L. Isolation of fungi from the dung of wild herbivores for application in bioethanol production. Braz J Microbiol.2017; 48: 648–655.
Sulman S, Rehman A . Isolation and Characterization of Cellulose Degrading Candida tropicalis W2 from Environmental Samples. Pakistan J. Zool. 2013; 45(3): 809-816.
Magalhaes C, Souza-Neto M, Astolfi-Filho S, Thiago I, Matos S. Candida tropicalis able to produce yeast single-cell protein using sugarcane bagasse hemicellulosic hydrolysate as carbon source .Biotechnol. Res. Innov. 2018; 2: 19-21.
Abdulla SK, Azzo NM. Two new records of Chaetomium species isolated from soil under grapevine plantations and a checklist of the genus in Iraq. J Agric Technol.2015; 4(2): 91-103.
Ellis D, Stephan D, Helen A ,Rosmary H, Roben B. Description of medical fungi .2nd ed. Astralia . 2007 ; 23-40.
Shukla P, Vishakarma P, Gawri S. Biotechnological potential of bacterial flora from Cheend juice: alcoholic beverage from Bastar. Indian Nat Sci. 2011; 9: 62-66.
Caputi A, Ueda MT, Brown T; Spectrophotometric determination of ethanol in wine. Am J Enol. Vitic. 1968; 19: 160-165.
Tesfaw A, Oner ET, Assefa F. Optimization of ethanol production using newly isolated ethanologenic yeasts. Biochem Biophys Rep. 2021; 25:100886. https://doi.org/10.1016/j.bbrep.2020.100886
Ireri N, Hamadi BI,Wanjiru W, Kachiru R. Characterization enzymatic activity and secondary metabolites of fungal isolates from lake Sonachi in Kenya. J. Pharm. Biol Sci . 2015 ;10(2): 65-76.
Oliveira A, Cunha M. Comparision of method for detection of biofilm production by coagulase –negative Staphylococci ., BMC.Res.Notes. 2010; 3: 260.
Kim S, Lee J, Sung B. Isolation and Characterization of the stress-tolerant Candida tropicalis YHJ1 and Evaluation of Its Xylose Reductase for Xylitol Production From Acid Pre-treatment Wastewater. Front Bioeng Biotechnol. 2019; 7: 138 https://doi.org/10.3389/fbioe.2019.00138
MakhuveleIgnatious R, Ncube Elbert N, Jansen van L, Daniël R, La Grange C. Isolation of fungi from the dung of wild herbivores for application in bioethanol production. Envir.Microbio. Braz J Microbiol. 2017; 48 (4): 648–655. https://doi.org/10.1016/j.bjm.2016.11.013
Abomughaid, MM, Isolation, and Identification of Fungi from Clinical Samples of Diabetic Patients and Studying the Anti-Fungal Activity of Some Natural Oils on Isolated Fungi; Baghdad Sci J. 2021; 18(3): 462-470.
Mussato SI, Machado EM, Carneiro LM, Teixeira JA. yme and yeast cells followed by pervaporation recovery of product – Kinetic model predictions. J Food Eng. 2007; 82: 618-625.
Rendon AR, Estradab CG, Castrillónc AA. Removal of water-soluble dye (methylene blue) by yeast Saccharomyces cerevisiae ;2020. https://repository.eafit.edu.co/bitstream/handle/10784/17083/Melissa_AcostaRendon_2020.pdf?sequence=2&isAllowed=y
Salem O, Abdelsalam A, Boroujerdi A . Bioremediation Potential of Chlorella vulgaris and Nostoc paludosum on azo Dyes with Analysis of Metabolite Changes. Baghdad Sci J. 2021; 18(3): 445-454.
Sunitha VH, Nirmala D., Srinivas C., Extracellular Enzymatic Activity of Endophytic Fungal Strains Isolated from Medicinal Plants. World J Agric Sci. 2016; 9(1): 1-9.
Touijer H, Benchemsi N, Ettayebi M, Idrissi AJ, Chaouni B, Bekkari H.Thermostable Cellulases from the Yeast Trichosporon sp., Enzyme Res 2019, ID 2790414, 6 pages. https://doi.org/10.1155/2019/2790414
Branda SS, Vik S, Friedman L, Kolter R. Biofilms: the matrix revisited. Trends Microbiol. 2015 ;13: 20. DOI: https://doi.org/10.1016/j.tim.2004.11.006.
Flemming HC, Wingender J. The biofilm matrix. Nat Rev Microbiol. 2015; 8: 623–633.
Yuan L, Hansen MF, Roder HL, Wang N, Burmolle M, He G. Mixed-species biofilms in the food industry: current knowledge and novel control strategies. Crit Rev Food Sci Nutr.2020; 60: 2277–2293.
Rajasekharan SK, Ramesh S. Cellulase inhibits Burkholderia cepacia biofilms on diverse prosthetic materials. Pol J Microbiol. 2013; 62 :327–330.
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