Spectrophotometric Determination of Salbutamol Sulphate and Isoxsuprine Hydrochloride in Pharmaceutical Formulations
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Abstract
A simple, sensitive and accurate spectrophotometric method has been developed for the determination of salbutamol sulphate (SAB) and isoxsuprine hydrochloride (ISX) in pure and pharmaceutical dosage. The method involved oxidation of (SAB) and (ISX) with a known excess of N-bromosuccinamid in acidic medium, and subsequent occupation of unreacted oxidant in decolorization of Evans blue dye (EB). This, in the presence of SAB or ISX was rectilinear over the ranges 1.0-12.0, 1.0-11.0 µg/mL, with molar absorptivity 4.21×104 and 2.58×104 l.mol-1.cm-1 respectively. The developed method had been successfully applied for the determination of the studied drugs in their pharmaceutical dosage resulting in a good agreement with certified value and standard addition procedure.
Received 5/1/2022
Accepted 14/4/2022
Published Online First 20/9/2022
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Tantucci C, Duguet A, Similowski T, Zelter M, Derenne J. Milic-Emili J. Effect of salbutamol on dynamic hyperinflation in chronic obstructive pulmonary disease patients. Eurp Res J. 1998; 12: 799-804.
Berger W. Levalbuterol: pharmacologic properties and use in the treatment of pediatric and adult asthma. Ann. Allergy Asthma In. 2003; 90(6):583-592.
Medina-Ruiz, Medina-Ruiz D, Erreguin-Luna B, Luna-Vazquez B, Romo-Mancillas A, Rojas-Molina A, et al. Vasodilation Elicited by Isoxsuprine, identified by High-Throughput Virtual Screening of Compound Libraries, Involves Activation of the NO/cGMP and H2S/KATP Pathways and Blockade of α1-Adrenoceptors and Calcium Channels. Molecules. 2019; 24(5):987.
Ghazi L, Khanzada M S, Munir A, Khan S, Rizwan M, Mehmood A. Effect of Modified Aspirin and Isoxsuprine Analogs on Ischemic Heart Disease. Adv. pharmacol. pharm. 2020; 8(3): 31-40.
Khoiwal S, Patidar V, Rastogi R, Tailor B. A comparative study between nifedipine and isoxsuprine in the suppression of preterm labor pain. Int J Reprod Contracept Obstet Gynecol. 2020; 9(7): 2886-2890.
HusseinK S, Ahmed A k, Mohammed F Y. Spectrophotometric determination of salbutamol by oxidative coupling reaction with 1-Naphthylamine-4-sulfonic acid in the presence of potassium per sulfate. Med. j. Babylon. 2021; 18(3): 249-256.
Al-Hashemi A H, AL-Mtwaiti S M. Spectrophotometric determination of salbutamol sulphate and mefenamic acid using azur-A dye in presence of oxidizing agent N-bromosuccinimide. J Edu Sci. 2020; 29(4): 222-244.
Hamoudi A T, Spectrophotometric assay of salbutamol sulphate in pharmaceutical preparations by coupling with diazotized ρ-bromoaniline. Baghdad Sci J. 2019; 16(1): 610-615.
Al-Salim L A, Al-Sharook M M. Indirect spectrophotometric determination of diclofenac and isoxsuprine drugs using N-bromosuccinimide and malachite green dye. J Edu Sci. 2019; 28(4): 18-32.
Nagle L C, Wahl A, Ogourstov V, Seymour I, Barry F, Rohan J F. Electrochemical discrimination of salbutamol from its excipients in ventolin TM at nanoporous gold microdisc arrays. Sensor. 2021; 21,3975.
Talay TP, Ali HS, Abdullah AA, Yardım Y, Şentürk Z. Electroanalytical determination of salbutamol in pharmaceutical formulations using cathodically pretreated boron-doped diamond electrode. Marmara Pharm J. 2018; 22 (3): 460-468.
Kelani K M, Elzanfaly E S, Halimc M K, Saad A S. Computational optimization of a novel solid-state sensor for stable assay of isoxsuprine hydrochloride in the presence of its nephrotoxic/hepatotoxic photothermal degradation products: application in different sampling matrices. New J Chem. 2020; 44(35): 15260-15269.
Shahrokhian S, Hafezi-Kahnamouei M. Glassy carbon electrode modified with a nanocomposite of multi-walled carbon nanotube decorated with Ag nanoparticles for electrochemical investigation of isoxsuprine. J Electroanal Chem. 2018; 825(15): 30-39.
Rele R V, Tiwatane P P. A validated potentiometric titration method for quantitative determination of isoxsuprine hydrochloride from pharmaceutical preparation. Asian J Res Chem. 2020; 13(6).
Kowsar S, Rubesh K S, Duganath N, Bharath R K P, Devanna N. A new RP-HPLC method development and validation for the simultanious estimation of salbutamol sulphate and theophyline in Pharmaceutical dosage form. Int J Pharm Res Sch. 2013; 2(3): 218-226.
Yang Y, Dong X Y, Li J, Ni H, Xue M, Zhao Z. Determination of Salbutamol Residues in Goat Various Tissues After Exposure to Growth-promoting Doses. J Anal Toxicol 2017; 42(1):55-62.
Korany M A, Fahmy O T, Mahgoub H, Maher H M. High performance liquid chromatographic determination of some guaiphenesin-containing cough-cold preparations. J Adv Res. 2011; 2:121-130.
Kelani K M, Elzanfaly E S, Saad A S, Halim M K, El-Zeiny M B. Different greenness assessment perspectives for stability-indicating RP-HPLC method used for the assay of isoxsuprine hydrochloride and four nephrotoxic and hepatotoxic photothermal degradation products. Microchem. J. 2021;171.
Aghwan M T, Salih E S. Indirect Spectrophotometric Determination of Cefalexin monohydrate, Ceftriaxone Sodium and Cefotaxim Sodium in Pharmaceuticals Using N-Bromosuccinimde and Evans Blue Dye. J Edu Sci.2020; 29(3):11-31.
Khopkar S M, Basic of Concepts of Analytical Chemistry. 2nd Ed.New Age Int. Ltd, New Delhi, India. 2004 :240P.
Little T A. Method Validation Essentials, Limit of Blank, Limit of Detection, and Limit of Quantitation. Bio Pharm Int. 2015; 28(4): 48-51.