GC-MS Quantification and Identification of Phytochemical Profiling, Potential Antioxidant Activity by DPPH, and Mineral Elements of Eggplant Powder (Solanum Melongena) in Sulaymaniyah City, Iraq

Authors

DOI:

https://doi.org/10.21123/bsj.2024.8766

Keywords:

Antioxidant activity, DPPH, Eggplant powder, GC/MS, ICP/OES, Mineral element.

Abstract

Solanum melongena, the plant used as a food and medical remedy known as eggplant, has anti-inflammatory, antioxidant, and anti-cancer properties. It is one of the vegetables that is most widely cultivated worldwide and has a wide range of vegetable sizes, colors, and shapes. This study looked at thirteen chemical compounds in the plant using gas chromatography and mass spectrometric detection (GC-MS). The gathered information verified that the object included thirteen chemical components, likes 2 — pentanone-4-hydroxy 4- methyl% 2.30, 1,3-dioxolane-4-methanol %0.17, formic acid %0.86, methenamine-methoxy %4.528,1,2,3-propanetriol (glycerin) %40.86, tetradecane%0.16, hexadecane%0.27, octadecane %0.16, myristic acid %3.14, nonadecane%0.44,9,12-octadecadienoic acid linolenic acid %0.43, oleic acid%2.36, and octacosane %0.62. These substances have been found to have strong anti-free radical properties, with eggplant powder's antioxidant activity standing out. These compounds are chemically characterized as antioxidants. Using the effective concentration EC50 method, the EC50 of eggplant powder (Solanum melongena) was 209 µg/mL. The extracts' DPPH-free radical scavenging activity was assessed, and it increased with concentration, going from 4.8828 (µg/mL) to 1250 (µg/mL). The radical scavenging activity of eggplant powder (Solanum melongena) increased from 0.87 to 76.04 indicating a high correlation between concentration and radical scavenging activity. The composition of the mineral elements in the powdered eggplant was ascertained using an (ICP/OES Perkin Elmer 2100), (Inductivity Coupled Plasma Emission Spectrometer). Al, Cu, Mn, Fe, Ba, Cr, Co, and Ni were found to be the principal minerals with high concentrations. However, the concentrations of (Cd 0.8974 µg/L and Pb not detectable) were incredibly low.

References

Wei Q, Wang J, Wang W, Hu T, Hu H, Bao C. A high-quality chromosome-level genome assembly reveals genetics for important traits in eggplant. Hortic Res. 2020; 7: 153. https://doi.org/10.1038/s41438-020-00391-0.

Mwinuka, P R, Mbilinyi BP, Mbungu W B, Mourice S K, Mahoo H F, Schmitter P. Optimizing water and nitrogen application for neglected horticultural species in tropical sub-humid climate areas: A case of African eggplant (Solanum aethiopicum L.). Sci Hortic. 2021; 276: 109756. https://doi.org/10.1016/j.scienta.2020.109756.

Sharma M, Kaushik P. Biochemical composition of eggplant fruits: A review. Appl Sci. 2021; 11(15): 7078. https://doi.org/10.3390/app11157078.

Naser J, Kadhim L, Sareea AR, Ahmed AR, Joseph C. Chemical Composition and antioxidant capacity of eggplant part during vegetative and flowering stage. J Phys Conf Ser. 2019; 1294. 092013. https://doi.org/10.1088/1742-6596/1294/9/092013

Maria JA , Isabel C F , Ferreira R, Joana D, Vânia T, Anabela M. A review on antimicrobial activity of mushroom (Basidiomycetes) extracts and isolated compounds. J Planta Med. 2012 ; 78(16): 1707-1718. https://doi.org/10.1055/s-0032-1315370

Jianqing L, Hongkun X, Junling L. Extraction of phenolics and anthocyanins from purple eggplant peels by multi-frequency ultrasound Effects of different extraction factors and optimization using uniform design. Ultrason Sonochem. 2022; 90 : 106174. https://doi.org/10.1016/j.ultsonch.2022.106174

Sofia C. Lourenço, Margarida Moldão-Martins and Vítor D. Alves. Antioxidants of Natural Plant Origins: From Sources to Food Industry Applications. Molecules. 2019; 24: 4132. https://doi.org/10.3390/molecules24224132

Akhbari M, Hamedi S, Aghamiri Z S. Optimization of total phenol and anthocyanin extraction from the peels of eggplant (Solanum melongena L.) and biological activity of the extracts. J Food Meas Charact. 2019; 13(4): 31833197. https://doi.org/10.1007/s11694-019-00241-1

Niño-medina G, Muy-rangel D, Gardea-béjar A, González-aguilar G, Heredia B, Báez-sañudo M. Nutritional and Nutraceutical Components of Commercial Eggplant Types Grown in Sinaloa Mexico. Not Bot Horti Agrobo Cluj Napoca, 2014; 42(2): 538-544. https://doi.org/10.15835/nbha4229573.

Scorsatto M, De- Castro P A, Da Silva A.J.R, Sabally K, Rosa G. Assessment of Bioactive Compounds, Physicochemical Composition, and In Vitro Antioxidant Activity of Eggplant Flour. Int J Cardiovasc Sci. 2017; 30(3): 235–242. https://doi.org/10.5935/2359-4802.20170046.

Hamzah RU, Agboota AR, Busari MB, Omogu EH, Umar MB, Abubakar A N. Evaluation of Hepatoprotective Effect of Methanol Extract of Solanum melongena on Carbon Tetrachloride Induced Hepatotoxic Rats. Eur J Med Plant. 2016; 13(3): 1-12. https://doi.org/10.9734/EJMP/2016/23473.

Mauro R P, Agenello M, Rizzo V, Graziani G, Fogliano V, Leonadi C. Recovery of Eggplant Field Waste as a Source of Phytochemical. Sci Hortc. 2020; 261: 109023. https://doi.org/10.1016/j.scienta.2019.109023.

Akanitapichat P, Phraibung K, Nuchklang K, Prompitakkul S. Antioxidant and Hepatoprotective Activities of Five Eggplant Varieties. Food Chem Toxicol. 2020 ; 48(10): 3017-3021. https://doi.org/10.1016/j.fct.2010.07.045

Laura Aracely CA, Aldo Moreno U,. Rommel A. Carballo C, Josefina León F, José Geovanni RQ. Metabolomic Analysis of Phytochemical Compounds from Agricultural Residues of Eggplant Solanum melongena L. Molecules. 2022; 27: 7013.https://doi.org/10.3390/molecules27207013.

Philippi K, Tsamandouras N, Grigorakis S, Makris D P. Ultrasound-Assisted Green Extraction of Eggplant Peel (Solanum melongena) Polyphenols Using Aqueous Mixtures of Glycerol and Ethanol Optimisation and Kinetics. Environ Process. 2016; 3(2): 369-386. https://doi.org/10.1007/s40710-016-0140-8.

Kalliopi I. Kadoglidou KK, Parthenopi R, Ifigeneia M, Apostolos K , Maria I. Assessing Physicochemical Parameters, Bioactive Profile and Antioxidant Status of Different Fruit Parts of Greek Eggplant Germplasm. Horticulturae . 2022; 8(12): 1113.https://doi.org/10.3390/horticulturae8121113.

Nino-Medina.G, Urias-Orona V, Muy-Rangel MD, Heredia JB. Structure and Content of Phenolics in Eggplant (Solanum Melongena) a review S Afr J Bot. 2017; 111: 161-169. https://doi.org/10.1016/j.sajb.2017.03.016.

Joo YK, Soo I L, Jin A K, Muthusamy M, Mi-Jeong J. Specific audible sound waves improve flavonoid contents and antioxidative properties of sprouts. Sci Hortic. 2021; 276: 109746. https://doi.org/10.1016/j.scienta.2020.109746.

Xian-kui M , Xiao-fei L, Jian-Yong ZL J, Wei-Wei L, Wang G. Analysis of the volatile components in Selaginella doederleinii by headspace solid phase micro extraction-gas chromatography-mass spectrometry. Molecules. 2020; 25(1): 115.https://doi.org/10.3390/molecules25010115

Batiha GE-S, Beshbishy AM, Adeyemi OS, Nadwa EH, Rashwan EM, Alkazmi LM. Phytochemical screening and antiprotozoal effects of the methanolic Berberis vulgaris and acetonic Rhus coriaria extracts. Molecules. 2020; 25(3): 550. https://doi.org/10.3390/molecules25030550.

Siddartha B, Riya M, Anjali P, Arpana P, Archana G, Ramendra P. Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Ficus religiosa. Molecules. 2022; 27(4): 1326. https://doi.org/10.3390/molecules27041326.

Trifa A O, Srwa NM, Trifa K J F, Suzan N A. Determination of Total Phenol, antioxidant and Antimicrobial Activity of Beetroot and Strawberry in Sulaimani City - Kurdistan Region Iraq. Egypt J Chem. 2022; 65(11): 583 -593.https://doi.org/10.21608/EJCHEM.2022.135832.5997.

Anitha IA, Rosaline V, David TT. Isolation and Characterization of Flavonoids (Rutin) from the Roots of Cadaba aphylla (Thunb) and Adenia glauca of Potential in Anti-Oxidant activity. Orient J Chem. 2022; 38(6): 1404-1413. http://dx.doi.org/10.13005/ojc/380610.

Silva D I J S, Lavorante A F, Paim A P S, Maria JDS. Microwave-assisted digestion employing diluted nitric acid for mineral determination in rice by ICP OES. Food Chem. 2020; 319: 126435. https://doi.org/10.1016/j.foodchem.2020.126435.

Bill L. Highly Unsaturated Fatty Acid (HUFA) Mediate and Monitors Impact on Health. J of Prostaglandins other Lipid Mediators. 2017; 133: 4-10. https://doi.org/10.1016/j.prostaglandins.2017.05.002.

Soobratte M A, Neergheen V S, Luximon-Ramma A, Aruoma O I, Bahorun T. Phenolics Potential Antioxidant Therapeutic Agents Mechanism and Action. Mutat Res. 2005; 579(1-2): 200-13. https://doi.org/10.1016/j.mrfmmm.2005.03.023.

Rosario PM, Michele A, Valeria R, Giulia G, Vincenzo F, Cherubino L. Recovery of eggplant field waste as a source of phytochemicals. Sci Hortic. 2020; 261: 109023. https://doi.org/10.1016/j.scienta.2019.109023.

Hanson PM, Yang R Y, Tsou SC S, Ledesma D, Engle L, Lee TC. Diversity on Eggplant (Solanum Melongena) for Superoxide Scavenging Activity, Total Phenolics and Ascorbic Acid. J Food Comps Anal. 2006; 19(6-7): 594-600. https://doi.org/10.1016/j.jfca.2006.03.001.

Ahmed A J, Kamaran KA, Parween A, Sharoukh M, Güldal M, Abdullah S S. Phytochemical profile, Antioxidant, Enzyme inhibitory and acute toxicity activity of Astragalus Bruguier. Baghdad Sci J. 2023; 20(1): 157-165. https://dx.doi.org/10.21123/bsj.2022.6769.

Monier M W, Wafaa M. H, Nesreen H. A. Using some Natural Minerals to Remove Cadmium from Polluted Water. Baghdad Sci J. 2022; 19(5): 1008-1013. http://dx.doi.org/10.21123/bsj.2022.5385.

Prohens J, Rodrıґguez-Burruezo A, Raigon M.D, Nuez F. Total phenolic concentration and browning susceptibility in a collection of different varietal types and hybrids of eggplant: implications for higher nutritional quality and reduced browning. J Am Soc Hortic Sci..2007; 132(5): 638–646. https://doi.org/10.21273/JASHS.132.5.638.

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GC-MS Quantification and Identification of Phytochemical Profiling, Potential Antioxidant Activity by DPPH, and Mineral Elements of Eggplant Powder (Solanum Melongena) in Sulaymaniyah City, Iraq. Baghdad Sci.J [Internet]. [cited 2024 Dec. 2];22(1). Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/8766