The expression of genes TLR2 and TLR10 in the gastric tissue of patients with gastroduodenal disorders caused by Helicobacter pylori
DOI:
https://doi.org/10.21123/bsj.2024.8962Keywords:
H. pylori, Gastritis, Gastric cancer, TLR2, TLR10 genes, Peptic ulcerAbstract
The bacterium Helicobacter pylori (H. pylori) is the key etiological agent in gastritis, peptic ulcer, and gastric cancer disease. Toll-like receptors (TLRs) contribute to the innate immune response through pathogen-associated molecular patterns (PAMPs) derived from H. pylori and other microorganisms as well as damage-associated molecular patterns (DAMPs) derived from host cells, such as tumour cells, dead or dying cells, or products released from cells in response to signals such as hypoxia. The study involved gastric biopsies taken from 88 gastroduodenal disorder patients and 21 healthy individuals. The clinical diagnoses of these groups after endoscopy examination included gastritis, peptic ulcers, and gastric cancer. The detection of H. pylori infection was performed using PCR to amplify ureC and ureA genes, then the bands were visualized by gel electrophoresis. The expression of TLR2 and TLR10 genes in gastric biopsies was evaluated using the relative method. The current findings indicate that susceptibility to gastroduodenal disorder increases with age, as most of the patients were over 30 years old (68 out of 88 patients) representing 77.2% of patients. The bacterium induced the expression of TLR2 and TLR10 genes, the gene TLR2 was recorded 11.11-fold in patients with gastritis, 7. 65-fold in those with peptic ulcer, and 6.25-fold in patients suffering from gastric cancer, the folding decreased with the prognosis of the disease. On the other hand, the TLR10 gene recorded the highest value 5.01-fold in peptic ulcer patients, followed by 4.73-fold with gastritis, and 3.10-fold with gastric cancer. The expression of the TLR2 and TLR10 genes was significantly higher (p ≤ 0.01) in the patients’ group compared with the control one. In gastritis, peptic ulcer, and gastric cancer patients, the TLR2 and TLR10 gene expression was higher than in the healthy group, and TLR2 was higher than TLR10.
Received 19/04/2023
Revised 12/08/2023
Accepted 14/08/2023
Published Online First 20/01/2024
References
Kuipers EJ. Exploring the link between Helicobacter pylori and gastric cancer. Review article: Aliment. Pharmacol. Ther. 1999; 13 Suppl 1: 3–11. https://doi.org/10.1046/j.1365-2036.1999.00002.x.
Saqari IML, Al-Ameri LMH. Biosensing technique for detection of H.pylori bacteria. Iraqi J Laser 2022; 21(2): 1-8.
Piscione M, Mazzone M, Di Marcantonio MC, Muraro R, Mincione G. Eradication of Helicobacter pylori and Gastric Cancer: a Controversial Relationship. Front Microbiol. 2021; 12: 1-18. https://doi.org/10.3389/fmicb.2021.630852.
Ferrero RL. Innate immune recognition of the extracellular mucosal pathogen, Helicobacter pylori. Mol Immunol. 2005; 42(8): 879–885. https://doi.org/10.1016/j.molimm.2004.12.001.
Uno K, Kato K, Shimosegawa T. Novel role of toll-like receptors in Helicobacter pylori-induced gastric malignancy. World J Gastroenterol. 2014; 20(18): 5244–5251. https://doi.org/10.3748/wjg.v20.i18.5244.
Mustafa AJ, Ismail PA. Association of potent inflammatory cytokine and oxidative DNA damage biomarkers in stomach cancer patients. Baghdad Sci J. 2022; 19(6): 1313-1325. https://doi.org/10.21123/bsj.2022.6589
Al-Sultan SASH, Al-Jubouri SHK. Haemagglutination (HA) test of Helicobacter pylori. Baghdad Sci J. 2014; 11(3): 1382-1386. https://doi.org/10.21123/bsj.2014.11.3.1382-1386
Kusters JG, van Vliet AH, Kuipers EJ. Pathogenesis of Helicobacter pylori infection. Clin Microbiol Rev. 2006; 19(3): 449–490. https://doi.org/10.1128/CMR.00054-05.
Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative CT method. Nat Protoc. 2008; 3(6): 1101-1108. https://doi.org/10.1038/nprot.2008.73.
Daniel WW, Cross, CL. Biostatistics, A Foundation for Analysis in the Health Sciences 2013; John Wiley and Sons. New York, P: 958.
Kalaf EA, Al-Khafaji ZM, Yassen NY, Al-Abbudi FA, Sadwen SN. Study of the cytotoxin-associated gene a (CagA gene) in Helicobacter pylori using gastric biopsies of Iraqi patients. Saudi J Gastroenterol. 2013; 19(2): 69–74. https://doi.org/10.4103/1319-3767.108474.
Salman, KD, Al-Thwaini AN, Askar BA. Evaluation of glmM Gene in Diagnosis of Helicobacter pylori with Another Invasive Method. IJB. 2019; 18(3): 16-25.
Shehab MJ, Abdul-Hassan IA, Mahdi BM. Prediction of risk factors in sample of Iraqi patients with developing gastritis. IJB. 2021; 1(20): 54–62.
Majumdar AP, Fligiel SE, Jaszewski R. Gastric mucosal injury and repair: effect of aging. Histol Histopathol. 1997; 12(2): 491–501. https://pubmed.ncbi.nlm.nih.gov/9151138/.
Schurz H, Salie M, Tromp G, Hoal EG, Kinnear CJ, Möller M. The X chromosome and sex-specific effects in infectious disease susceptibility. Hum Genomics. 2019; 13(1): 1-9. https://doi.org/10.1186/s40246-018-0185-z.
Zhu HM, Li BY, Tang Z, She J, Liang XY, Dong LK, et al. Epidemiological investigation of Helicobacter pylori infection in elderly people in Beijing. World J Clin Cases. 2020; 8(11): 2173–2180. https://doi.org/10.12998/wjcc.v8.i11.2173.
Majeed PD, Khoshnaw KJS. Seroprevalence of Helicobacter pylori infection among patients with gastroduodenal disorders in Erbil city. DJM. 2020; 18(1): 91-101.
Lafta SA, AL-Faisal AM. Association of Helicobacter pylori Infection and Gastric Cancer. IJB. 2017; 16(1): 1-9.
Mobley HL. Urease. Helicobacter pylori: physiology and genetics. 2001.21:177-91. https://doi.org/10.1128/9781555818005.ch16
Allen MG, Bate MY, Tramonte LM, Avalos EY, Loh J, Cover TL, et al. Regulation of Helicobacter pylori Urease and Acetone Carboxylase genes by nitric oxide and the CrdRS two-component system. Microbiol Spectr. 2023; 11(1), Suppl 1: 56–62. https://doi.org/10.1128/spectrum.04633-2.
De Reuse H, Labigne A, Mengin-Lecreulx D. The Helicobacter pylori ureC gene codes for a phosphoglucosamine mutase. J Bacteriol. 1997; 179(11): 3488–3493. https://doi.org/10.1128/jb.179.11.3488-3493.1997.
Nagashima H, Iwatani S, Cruz M, Jiménez Abreu JA, Uchida T, Mahachai V, et al. Toll-like Receptor 10 in Helicobacter pylori Infection. J Infect Dis. 2015; 212(10): 1666–1676. https://doi.org/10.1093/infdis/jiv27.
McClure R, Massari P. TLR-dependent human mucosal epithelial cell responses to microbial pathogens. Front Immunol. 2014; 5: 386-392. https://doi.org/10.3389/fimmu.2014.00386.
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