In Vivo Study the Cytogenetic Effect of Ammi majus Methanolic Extract on Mitotic Index, Micronucleus Formation and DNA Damage on Mitoxantrone Treated Albino Male Mice
DOI:
https://doi.org/10.21123/bsj.2024.9034Keywords:
Anticancer, Male mouse, Medicinal plant, Metaphase index, Methanolic extractAbstract
Medicinal plants or their secondary metabolites have shown different biological effects with a wide range of pharmacological properties; for instance, immune stimulator, anti-bacterial, anti-viral, anti-inflammatory, anti-oxidant, anti-mutagenic, anticancer, hepatoprotective, and many other properties. One of these medicinal plants is Ammi majus. The purpose of this research was to evaluate the cytogenetic impact of a methanolic extract of the Ammi majus plant on micronucleus formation, mitotic index and DNA damage in mice that had been given intraperitoneally mitoxantrone medicine (anticancer drug). Mice were divided into four groups, each group consisting of four mice: group I (positive control), mice treated with 0.008 mg/mouse mitoxantrone, group II (negative control), mice not given any treatment, group III, and group IV (interaction groups), mice treated with 50 mg/kg and 100 mg/kg of plant extract three through seven, respectively, after receiving injections of the mitoxantrone drug on days one and two. The findings showed that, in comparison to positive and negative controls, the plant has the ability to induce the mitotic index to 10.67±2.33% and 12.65± 3.01% for 50 and 100 mg\kg respectively, compared to 5.31±1.33% for mitoxantrone treated mice. In addition, the results of micronucleus formation indicated the capacity of the plant to reduce its formation to 2.63 ±0.011 and 2.47±0.012 micronucleus\cell after treating the mice with Ammi majus two doses (50 and 100 mg\kg), in comparison to the drug that enhances micronucleus formation to (4.28± 0.00 micronucleus\cell). DNA damage results explained the ability of the plant to reduce DNA tail damage to 134 ±22.3 and 117 ±16.1% for 50 and 100 mg/kg of Ammi majus extract, respectively which increased to (176 ±11.23%) after mice were treated with drugs. All these findings may be attributed to plant-active compounds such as flavonoids and other constituents.
Received 06/05/2023
Revised 08/09/2023
Accepted 10/09/2023
Published Online First 20/01/2024
References
Shalash WK, Al-Ezzy RM, Ahmaed AS. Antitumor activity of dextran produced from localized leuconostoc mesenteroides isolates. Biochem. Cell Arch. 2021 Oct; 21(1): 2371-2374: 2021,
Fathallah N, Raafat MM, Issa MY, Abdel-Aziz MM, Bishr M, Abdelkawy MA, et al. Bio-guided fractionation of prenylated benzaldehyde derivatives as potent antimicrobial and antibiofilm from Ammi majus L. fruits-associated Aspergillus amstelodami. Molecules. 2019 Nov; 24(22): 118. https://doi.org/10.3390/molecules24224118.
Miara M, Bendif H, Rebbas K, Rabah B, Hammou M, Maggi F. Medicinal plants and their traditional uses in the highland region of Bordj Bou Arreridj (Northeast Algeria). J Herb Med. 2019 June; 16: 100262. https://doi.org/10.1016/j.hermed.2019.100262.
Kausar H, Abidin L, Mujeeb M, Aqil M, Alam O. Factorial design-guided optimization of extraction of therapeutically active furanocoumarin khellin from Ammi majus L. fruits. Pharmacogn Mag. 2020 Jan; 16(72): 835. https://doi.org/10.4103/pm.pm_295_20.
Hossain MA, Al Touby S. Ammi majus an Endemic Medicinal Plant: A Review of the Medicinal Uses, Pharmacological and Phytochemicals. Ann Toxicol. 2020 Oct; 2(1): 9–14. https://doi.org/10.36959/736/634.
Arafah MW, Almutairi B, Al-Zharani M, Alkahtane AA, Al-Otibi FO, Ali D, et al. The protective effect of Ammi visnaga extract against human hepatic cancer. J King Saud Univ Sci. 2021 Sep; 33(6):101540. https://doi.org/10.1016/j.jksus.2021.101540.
Usmani OI, Jahan N, Aleem M, Hasan SA. Aatrilal (Ammi majus L.), an important drug of Unani system of medicine: A review. J Ethnopharmacol. 2021 Aug; 276:114-144. https://doi.org/10.1016/j.jep.2021.114144.
Hussain H, Al-Harrasi A, Al-Rawahi A, Green IR, Csuk R, Ahmed I, et al.A fruitful decade from 2005 to 2014 for anthraquinone patents. Expert Opin Ther Pat. 2015 Jun; 25(9): 1053-64. https://doi.org/10.1517/13543776.2015.1050793.
Enache M, Toader AM, Enache MI. Mitoxantrone-Surfactant Interactions: A Physicochemical Overview. Molecules.2016 Oct 13; 21(10): 1356. https://doi.org/10.3390/molecules21101356.
Taskeen A, Naeem I, Mubeen H, Mehmood T. Reverse phase high performance liquid chromatographic analysis of flavonoids in two Ficus species. New York Sci J. 2009 Sep; 2(5): 32-35. https://doi.org/10.7537/marsnys020509.06
Al-ezzy RM, Alanee R SH, Khalaf HM. Cytogenetic and Cytotoxic Potentials of Borago Officinalis on Albino Male Mice. Iraqi J Sci. 2022 Sep; 63(9): 3703-3710. https://doi.org/10.24996/ijs.2022.63.9.4.
Allen JW, CV Shuler, RW Mendes, Latt SA. A simplified technique for in vivo analysis of sister chromatid exchanges using 5-bromo-deoxy uridine tablets. Cytogenet Cell Genet. 1977; 18 (4): 231–237. https://doi.org/10.1159/000130765.
Schmid, W. The cell micronucleus test for cytogenes analysis in: Hollaender, A. (Ed.) Chemical Mutagens principles and Methods for their Detection. . Mutat Res.1976 Feb; 4: 31-53. https://doi.org/10.1016/0165-1161(75)90058-8
Al-Shmgani HS, Moate RM, Sneyd J, Macnaughton P, Moody A. Hyperoxia-induced ciliary loss and oxidative damage in an in vitro bovine model: the protective role of antioxidant vitamins E and C. Biochem Biophys Res Commun, 2012 Dec; 429(3-4): 191-196. https://doi.org/10.1016/j.bbrc.2012.10.113
Hossain MA, Touby SA. Ammi majus an endemic medicinal plant: a review of the medicinal uses, pharmacological and phytochemicals. Ann Toxicol. 2020 Oct; 2(1): 9–14. https://doi.org/10.36959/736/634
Mirzaei SA, Gholamian DN, Ghamghami M, Amiri AH, Dalir Abdolahinia E, Elahian F. ABC-transporter blockage mediated by xanthotoxin and bergapten is the major pathway for chemosensitization of multidrug-resistant cancer cells. Toxicol Appl Pharmacol. 2017 Oct; 15 (337): 22–29. https://doi.org/10.1016/j.taap.2017.10.018.
El-Sharkawy E, Selim Y. Three new coumarin types from aerial parts of Ammi majus L. and their cytotoxic activity. Z Naturforsch C J Biosci. 2018 Jan 1; 73(1-2): 1-7. https://doi.org/10.1515/znc-2017-0068.
Deng X, Zhao X, Lan Z, Jiang J, Yin W, Chen L. Anti-tumor effects of flavonoids from the ethnic medicine Docynia delavayi (Franch.) Schneid. and its possible mechanism. J Med Food. 2014 Jul; 17(7): 787-94. https://doi.org/10.1089/jmf.2013.2886. Epub 2014 Jun 18. PMID: 24940817.
Rathee P, Chaudhary H, Rathee S, Rathee D, Kumar V, Kohli K. Mechanism of action of flavonoids as anti-inflammatory agents: a review. Inflamm Allergy Drug Targets. 2009 Jul; 8(3): 229-35. https://doi.org/10.2174/187152809788681029. PMID: 19601883.
Zhang Z, Yang L, Hou J, Tian S, Liu Y. Molecular mechanisms underlying the anticancer activities of licorice flavonoids. J Ethnopharmacol. 2021 Mar; 267(1):113635. https://doi.org/10.1016/j.jep.2020.113635. Epub 2020 Nov 25. PMID: 33246112.
Jacobs H, Moalin M, Bast A, van der Vijgh WJ, Haenen GR. An essential difference between the flavonoids monoHER and quercetin in their interplay with the endogenous antioxidant network. PLoS One. 2010 Nov 8; 5(11): e13880. https://doi.org/10.1371/journal.pone.0013880.
Takemura H, Sakakibara H, Yamazaki S, Shimoi K. Breast cancer and flavonoids - a role in prevention. Curr Pharm Des. 2013; 19(34): 6125-32. https://doi.org/10.2174/1381612811319340006. PMID: 23448447.
Eliaa SG, Al-Karmalawy AA, Saleh RM, Elshal MF. Empagliflozin and Doxorubicin Synergistically Inhibit the Survival of Triple-Negative Breast Cancer Cells via Interfering with the mTOR Pathway and Inhibition of Calmodulin: In Vitro and Molecular Docking Studies. ACS Pharmacol Transl Sci. 2020 Dec; 3 (6): 1330–1338.https://doi.org/10.1021/acsptsci.0c00144.
Khalil N, Bishr M, Desouky S, Salama O. Ammi Visnaga L. a Potential Medicinal Plant: A Review. Molecules. 2020 Jan; 25(2): 301. https://doi.org/10.3390/molecules25020301
Mirzaei SA, Gholamian DN, Ghamghami M, Amiri AH, Dalir Abdolahinia E, Elahian F. ABC-transporter blockage mediated by xanthotoxin and bergapten is the major pathway for chemosensitization of multidrug-resistant cancer cells. Toxicol Appl Pharmacol. 2017 Dec; 337: 22–29. https://doi.org/10.1016/j.taap.2017.10.018.
Ad’hiah, A H, Ibraheem, RM, Abbood, KW. Modulation of cytokine production from cultured mononuclear cells of leukemia patients by Hypericum triquetrifolium Turra methanolic extract. Arab J Basic Appl Sci. 2018 Apr; 25(1): 3–19. https://doi.org/10.1080/25765299.2018.1449413
Issa MY, Elshal MF, Fathallah N, Abdelkawy M, Bishr M , Salama O, et al. Potential Anticancer Activity of the Furanocoumarin Derivative Xanthotoxin Isolated from Ammi majus L. Fruits: In Vitro and In Silico Studies. Molecules. 2022 Jan; 27(3): 943. https://doi.org/10.3390/molecules27030943
Mohammed MM, El-Sharkawy ER. Cytotoxic new furoquinoline alkaloid isolated from Ammi majus L. growing in Egypt. Nat Prod Res. 2017 Mar; 31(6):645-652. https://doi.org/10.1080/14786419.2016.1217858
Adımcılar V, Beyazit N, Bedia EF. Khellin and visnagin in different organs of Ammi visnaga and Ammi majus. Nat Prod Res. 2023 Jan; 37(1): 164-166. https://doi.org/10.1080/14786419.2021.1956924
Koriem KMM, Asaad GF, Megahed H A, Zahran H, Arbid MS. Evaluation of the antihyperlipidemic, anti-inflammatory, analgesic, and antipyretic activities of ethanolic extract of Ammi majus seeds in albino rats and mice. Int J Toxicol. 2012 Jun; 31(3): 294-300. https://doi.org/10.1177/1091581812440889
Al-Ezzy R M, Alshanon A F, Khalaf H M. Studying some cytotoxic and cytogenetic potentials of Dandelion methanolic extract on MCF-7 cancer cell line: an in vitro study. Iraqi J Sci. 2023 Fab ;64(3): 1160–1170. https://doi.org/10.24996/ijs.2023.64.3.12.
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