دراسة التاثير الوراثي لنبات الخله داخل جسم الكائن الحي على معامل الانقسام الخلوي,تكوين النوى الصغيرة و تحطم الدنا الوراثي (DNA )

المؤلفون

  • Ruqaya Mohammed Al-ezzy قسم التقنيات الحيوية الجزيئيه والطبية، كلية التقنيات الاحيائية، جامعه النهرين، بغداد، العراق. https://orcid.org/0000-0002-7168-7327
  • Maysaa Hamid Ahmed قسم التقنيات الحيوية النباتية ، كلية التقنيات الاحيائية ,جامعه النهرين، بغداد، العراق. https://orcid.org/0000-0002-8779-897X
  • Hadeel Mohamed Khalaf قسم التقنيات الحيوية الجزيئيه والطبية، كلية التقنيات الاحيائية، جامعه النهرين، بغداد، العراق. https://orcid.org/0000-0001-8684-0905

DOI:

https://doi.org/10.21123/bsj.2024.9034

الكلمات المفتاحية:

مضادات السرطان، المستخلص الميثانولي، النباتات الطبية، ذكر الفأر، معامل الانقسام الاستوائي

الملخص

صممت هذه الدراسة للكشف عن التاثير الوراثي للمستخلص الميثانولي لنبات الخلة على معامل الانقسام الخلوي,تكون النوى الصغيرة و مدى تاثيره على تحطم الدنا الوراثي في الفئران المستحثة بعقار الميتوزانترون (المضاد للسرطان). استخدمت في هذه الدراسة اربعه مجاميع للفئران: المجموعه الاولى(السيطرة الموجبة )الفئران المعاملة بعقار الميتوزانترون بتركيز (0,008 ملغم\فارة) والمجموعه الثانية (السيطرة السالبة) الفئران الغير معامله اما المجوعه الثالثة والرابعه كانت الفئران المعاملة بالعقار لليومين الاول والثاني ومن اليوم 3 الى 7 عوملت الفئران بالمسخلص النباتي يتركيزي (50 و 100 ملغم\كغم) على التوالي. اظهرت النتائج قابلية النبات على زيادة الانقسام الخلوي بالمقارنه مع السيطرة الموجبة والسالبة كما اظهرت قابلية النبات على خفض نسبة تكون النوى الصغيرة وتحطم الدنا بالمقارنه ايضا مع السيطرتين الموجبة والسالبة.

المراجع

Shalash WK, Al-Ezzy RM, Ahmaed AS. Antitumor activity of dextran produced from localized leuconostoc mesenteroides isolates. Biochem. Cell Arch. 2021 Oct; 21(1): 2371-2374: 2021,

Fathallah N, Raafat MM, Issa MY, Abdel-Aziz MM, Bishr M, Abdelkawy MA, et al. Bio-guided fractionation of prenylated benzaldehyde derivatives as potent antimicrobial and antibiofilm from Ammi majus L. fruits-associated Aspergillus amstelodami. Molecules. 2019 Nov; 24(22): 118. https://doi.org/10.3390/molecules24224118.

Miara M, Bendif H, Rebbas K, Rabah B, Hammou M, Maggi F. Medicinal plants and their traditional uses in the highland region of Bordj Bou Arreridj (Northeast Algeria). J Herb Med. 2019 June; 16: 100262. https://doi.org/10.1016/j.hermed.2019.100262.

Kausar H, Abidin L, Mujeeb M, Aqil M, Alam O. Factorial design-guided optimization of extraction of therapeutically active furanocoumarin khellin from Ammi majus L. fruits. Pharmacogn Mag. 2020 Jan; 16(72): 835. https://doi.org/10.4103/pm.pm_295_20.

Hossain MA, Al Touby S. Ammi majus an Endemic Medicinal Plant: A Review of the Medicinal Uses, Pharmacological and Phytochemicals. Ann Toxicol. 2020 Oct; 2(1): 9–14. https://doi.org/10.36959/736/634.

Arafah MW, Almutairi B, Al-Zharani M, Alkahtane AA, Al-Otibi FO, Ali D, et al. The protective effect of Ammi visnaga extract against human hepatic cancer. J King Saud Univ Sci. 2021 Sep; 33(6):101540. https://doi.org/10.1016/j.jksus.2021.101540.

Usmani OI, Jahan N, Aleem M, Hasan SA. Aatrilal (Ammi majus L.), an important drug of Unani system of medicine: A review. J Ethnopharmacol. 2021 Aug; 276:114-144. https://doi.org/10.1016/j.jep.2021.114144.

Hussain H, Al-Harrasi A, Al-Rawahi A, Green IR, Csuk R, Ahmed I, et al.A fruitful decade from 2005 to 2014 for anthraquinone patents. Expert Opin Ther Pat. 2015 Jun; 25(9): 1053-64. https://doi.org/10.1517/13543776.2015.1050793.

Enache M, Toader AM, Enache MI. Mitoxantrone-Surfactant Interactions: A Physicochemical Overview. Molecules.2016 Oct 13; 21(10): 1356. https://doi.org/10.3390/molecules21101356.

Taskeen A, Naeem I, Mubeen H, Mehmood T. Reverse phase high performance liquid chromatographic analysis of flavonoids in two Ficus species. New York Sci J. 2009 Sep; 2(5): 32-35. https://doi.org/10.7537/marsnys020509.06

Al-ezzy RM, Alanee R SH, Khalaf HM. Cytogenetic and Cytotoxic Potentials of Borago Officinalis on Albino Male Mice. Iraqi J Sci. 2022 Sep; 63(9): 3703-3710. https://doi.org/10.24996/ijs.2022.63.9.4.

Allen JW, CV Shuler, RW Mendes, Latt SA. A simplified technique for in vivo analysis of sister chromatid exchanges using 5-bromo-deoxy uridine tablets. Cytogenet Cell Genet. 1977; 18 (4): 231–237. https://doi.org/10.1159/000130765.

Schmid, W. The cell micronucleus test for cytogenes analysis in: Hollaender, A. (Ed.) Chemical Mutagens principles and Methods for their Detection. . Mutat Res.1976 Feb; 4: 31-53. https://doi.org/10.1016/0165-1161(75)90058-8

Al-Shmgani HS, Moate RM, Sneyd J, Macnaughton P, Moody A. Hyperoxia-induced ciliary loss and oxidative damage in an in vitro bovine model: the protective role of antioxidant vitamins E and C. Biochem Biophys Res Commun, 2012 Dec; 429(3-4): 191-196. https://doi.org/10.1016/j.bbrc.2012.10.113

Hossain MA, Touby SA. Ammi majus an endemic medicinal plant: a review of the medicinal uses, pharmacological and phytochemicals. Ann Toxicol. 2020 Oct; 2(1): 9–14. https://doi.org/10.36959/736/634

Mirzaei SA, Gholamian DN, Ghamghami M, Amiri AH, Dalir Abdolahinia E, Elahian F. ABC-transporter blockage mediated by xanthotoxin and bergapten is the major pathway for chemosensitization of multidrug-resistant cancer cells. Toxicol Appl Pharmacol. 2017 Oct; 15 (337): 22–29. https://doi.org/10.1016/j.taap.2017.10.018.

El-Sharkawy E, Selim Y. Three new coumarin types from aerial parts of Ammi majus L. and their cytotoxic activity. Z Naturforsch C J Biosci. 2018 Jan 1; 73(1-2): 1-7. https://doi.org/10.1515/znc-2017-0068.

Deng X, Zhao X, Lan Z, Jiang J, Yin W, Chen L. Anti-tumor effects of flavonoids from the ethnic medicine Docynia delavayi (Franch.) Schneid. and its possible mechanism. J Med Food. 2014 Jul; 17(7): 787-94. https://doi.org/10.1089/jmf.2013.2886. Epub 2014 Jun 18. PMID: 24940817.

Rathee P, Chaudhary H, Rathee S, Rathee D, Kumar V, Kohli K. Mechanism of action of flavonoids as anti-inflammatory agents: a review. Inflamm Allergy Drug Targets. 2009 Jul; 8(3): 229-35. https://doi.org/10.2174/187152809788681029. PMID: 19601883.

Zhang Z, Yang L, Hou J, Tian S, Liu Y. Molecular mechanisms underlying the anticancer activities of licorice flavonoids. J Ethnopharmacol. 2021 Mar; 267(1):113635. https://doi.org/10.1016/j.jep.2020.113635. Epub 2020 Nov 25. PMID: 33246112.

Jacobs H, Moalin M, Bast A, van der Vijgh WJ, Haenen GR. An essential difference between the flavonoids monoHER and quercetin in their interplay with the endogenous antioxidant network. PLoS One. 2010 Nov 8; 5(11): e13880. https://doi.org/10.1371/journal.pone.0013880.

Takemura H, Sakakibara H, Yamazaki S, Shimoi K. Breast cancer and flavonoids - a role in prevention. Curr Pharm Des. 2013; 19(34): 6125-32. https://doi.org/10.2174/1381612811319340006. PMID: 23448447.

Eliaa SG, Al-Karmalawy AA, Saleh RM, Elshal MF. Empagliflozin and Doxorubicin Synergistically Inhibit the Survival of Triple-Negative Breast Cancer Cells via Interfering with the mTOR Pathway and Inhibition of Calmodulin: In Vitro and Molecular Docking Studies. ACS Pharmacol Transl Sci. 2020 Dec; 3 (6): 1330–1338.https://doi.org/10.1021/acsptsci.0c00144.

Khalil N, Bishr M, Desouky S, Salama O. Ammi Visnaga L. a Potential Medicinal Plant: A Review. Molecules. 2020 Jan; 25(2): 301. https://doi.org/10.3390/molecules25020301

Mirzaei SA, Gholamian DN, Ghamghami M, Amiri AH, Dalir Abdolahinia E, Elahian F. ABC-transporter blockage mediated by xanthotoxin and bergapten is the major pathway for chemosensitization of multidrug-resistant cancer cells. Toxicol Appl Pharmacol. 2017 Dec; 337: 22–29. https://doi.org/10.1016/j.taap.2017.10.018.

Ad’hiah, A H, Ibraheem, RM, Abbood, KW. Modulation of cytokine production from cultured mononuclear cells of leukemia patients by Hypericum triquetrifolium Turra methanolic extract. Arab J Basic Appl Sci. 2018 Apr; 25(1): 3–19. https://doi.org/10.1080/25765299.2018.1449413

Issa MY, Elshal MF, Fathallah N, Abdelkawy M, Bishr M , Salama O, et al. Potential Anticancer Activity of the Furanocoumarin Derivative Xanthotoxin Isolated from Ammi majus L. Fruits: In Vitro and In Silico Studies. Molecules. 2022 Jan; 27(3): 943. https://doi.org/10.3390/molecules27030943

Mohammed MM, El-Sharkawy ER. Cytotoxic new furoquinoline alkaloid isolated from Ammi majus L. growing in Egypt. Nat Prod Res. 2017 Mar; 31(6):645-652. https://doi.org/10.1080/14786419.2016.1217858

Adımcılar V, Beyazit N, Bedia EF. Khellin and visnagin in different organs of Ammi visnaga and Ammi majus. Nat Prod Res. 2023 Jan; 37(1): 164-166. https://doi.org/10.1080/14786419.2021.1956924

Koriem KMM, Asaad GF, Megahed H A, Zahran H, Arbid MS. Evaluation of the antihyperlipidemic, anti-inflammatory, analgesic, and antipyretic activities of ethanolic extract of Ammi majus seeds in albino rats and mice. Int J Toxicol. 2012 Jun; 31(3): 294-300. https://doi.org/10.1177/1091581812440889

Al-Ezzy R M, Alshanon A F, Khalaf H M. Studying some cytotoxic and cytogenetic potentials of Dandelion methanolic extract on MCF-7 cancer cell line: an in vitro study. Iraqi J Sci. 2023 Fab ;64(3): 1160–1170. https://doi.org/10.24996/ijs.2023.64.3.12.

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كيفية الاقتباس

1.
دراسة التاثير الوراثي لنبات الخله داخل جسم الكائن الحي على معامل الانقسام الخلوي,تكوين النوى الصغيرة و تحطم الدنا الوراثي (DNA ). Baghdad Sci.J [انترنت]. [وثق 17 مايو، 2024];21(8). موجود في: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/9034