Evaluation of Retinol Binding Protein 4 Level in Iraqi Patients with Type 2 Diabetes and Pre-diabetes Status as a Predictive Factor

Authors

  • Heba Mohammed Hatem Department of Chemistry, College of Science for Women, University of Baghdad, Baghdad, Iraq. https://orcid.org/0009-0004-8122-8142
  • Kadhim K. Ghudhaib Department of Chemistry, College of Science for Women, University of Baghdad, Baghdad, Iraq.

DOI:

https://doi.org/10.21123/bsj.2024.9088

Keywords:

Diabetes mellitus II, HOMA-IR, Insulin, Pre-diabetes, Retinol binding protein 4.

Abstract

The prevalence of type 2 diabetes has doubled in recent years due to a defect in insulin production, which can develop to form diabetes complications that affect the kidneys, nerves, and eyes. As a result, early diagnosis and classification of Type II diabetes are critical to aiding physician assessments. Accordingly, the current study aimed to determine Retinol binding protein 4 (RBP4) levels in patients with T2DM and pre-DM as early predictors for disease cases. The current study included 138 subjects, divided into three groups, on the basis of FBG and HbA1c criteria, including (DM, 46 patients, pre-DM, 46 participants, and healthy subject,46 persons), when they enrolled in the National Diabetes Center- Al-Mustansiriya University. Enzyme-linked immunosorbent assays (ELISA) were used to detect the levels of Retinol Binding Protein 4 and Insulin, and spectrophotometric techniques were used to determine the levels of FBG and lipid profile.  Retinol binding protein 4 results revealed significant differences (P≤0.0001) among the studied groups. Also, the insulin results showed significant differences (P≤0.0001) between the diabetic and control groups. The results of HOMA-IR showed significant differences (P≤0.0001). It also showed a lipid profile significant effect (P≤0.0022), (P≤0.0001), (P≤ 0.050) between the studied groups, except HDL that showed no significant difference(P≤.0.148). In conclusion, retinol-binding protein 4 can serve as an early indicator of T2DM, and this conclusion can be reinforced by the results of the ROC analysis, which indicated that Retinol binding protein 4 is an excellent indicator for diagnosis of the studied condition.

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Evaluation of Retinol Binding Protein 4 Level in Iraqi Patients with Type 2 Diabetes and Pre-diabetes Status as a Predictive Factor. Baghdad Sci.J [Internet]. [cited 2024 Dec. 6];22(1). Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/9088