Endothelin-1 Gene Polymorphism with Interleukin-1β Expression in Infertile Iraqi Women under In Vitro Fertilization Program
DOI:
https://doi.org/10.21123/bsj.2024.9275Keywords:
EDN1 gene, EDN1 gene expression, IL-1β gene, Polymorphism, Infertile Iraqi Women, IVF programAbstract
Endometrial receptivity is the limiting phase in the success of IVF treatment, Invasion of human trophoblast cells to the endometrial is regulated by many factors such as EDN1 and IL-1β. This study aims to verify the relationship between polymorphism and gene expression of EDN1 and IL-1β genes with embryo implantation in infertile women under the IVF program. The peripheral blood samples were collected on the day of embryo transfer an hour after embryo transfer, after approval from 60 women, and divided according to the outcome of embryo implantation outcomes, the failure implantation group included 35 women, and the success implantation group included 25 women. The results of sequencing revealed two genetic variants in the EDN1 gene, SNP rs2070699 showed no association with the embryo implantation outcome in infertility women according to Fisher probability. SNP rs2070699 shows a higher frequency of genotypes GG and TT than the GT genotype in the study groups. Variant No.7196T>G on exon 2 shows that the TT genotype had the highest frequency in the success group, while the TG genotype recorded the highest frequency in the failure group. The results of EDN1 and IL-1β gene expression fold change showed non-significant differences between groups. The correlation between EDN1, and IL-1β gene expression showed a significant positive relationship. In conclusion, Genetic variants of the SNPs rs2070699 and No.7196 in EDN1 do not affect the gene expression level in infertility women under the IVF program. EDN1 and IL-1β gene expression have a significant positive relationship in the luteal phase and both are upregulated in women with successful implantation.
Received 27/06/2023
Revised 22/09/2023
Accepted 24/09/2023
Published Online First 20/07/2024
References
Daud N, Gustia K. In-Vitro Fertilizatiion (IVF) Concept According to Modern Medical. IJ-ARBSS. 2023; 13; 6: 1254-1260. http://dx.doi.org/10.6007/IJARBSS/v13-i6/17571.
Afnan MA, Rudin C, Conitzer V, Savulescu J, Mishra A, Liu Y, et al. Ethical implementation of artificial intelligence to select embryos in in vitro fertilization. In Proceedings of the 2021 AAAI/ACM Conference on AI, Ethics Soc. 2021; 316-326. https://doi.org/10.1145/3461702.3462589.
Karizbodagh MP, Rashidi B, Sahebkar A, Masoudifar A, Mirzaei H. Implantation window and angiogenesis. J Cell Biochem. 2017; 118(12): 4141-51. https://doi.org/10.1002/jcb.26088.
Hertig AT, Rock J, Adams EC. A description of 34 human ova within the first 17 days of development. Am J Anat. 1956; 98(3): 435-93. https://doi.org/10.1002/aja.1000980306.
Maia Bezerra NK, de Menezes Galvao AC, Martins Leite NE, Leão Barbalho Sant’anna A, de Medeiros Garcia Torres M, Galvao Pinto Coelho MC, et al. Success of in vitro fertilization and its association with the levels of psychophysiological stress before and during the treatment. Health Care Women Int. 2021; 42(4-6): 420-45. https://doi.org/10.1080/07399332.2020.1787415.
Singh M, Chaudhry P, Asselin E. Bridging endometrial receptivity and implantation: network of hormones, cytokines, and growth factors. J Endocrinol. 2011; 210(1): 5-14. https://doi.org/10.1530/joe-10-0461.
Hassab ZS, Almohaidi AMS, Alsaidi FS. Association between the Genetic Variation of LIF R Gene with Its Gene Expression and Implantation Outcomes of IVF-Iraqi Arab Females. J Pharm Negat. 2022; 13(4): 1665- 1671. http://dx.doi.org/10.47750/pnr.2022.13.04.230
Yousif RA, Almohaidi AMS, Al-Musawi BJ. Assessment of ITGB3 gene expression and hormonal status in infertile female undergoing an in vitro fertilization protocol. HIV Nursing. 2023; 23(1): 770-8.
Yousif RA, Almohaidi AM. Identification of ITGB3 Polymorphisms with implantation outcome during in vitro fertilization program. HIV Nursing. 2023; 23(1): 257-64. https://doi.org/10.31838/hiv23.01.42.
Mirza SF, Saeed RH, Almohaidi AMS, Al-Janabi IJ. Evaluation mucin 1 polymorphism and expression with infertility in Iraqi females. Res Mil. 2022; 14; 12(2): 6916-27.
Mihanfar A, Sadigh AR, Fattahi A, Latifi Z, Hasanzadeh‐Moghadam M, Samadi M, et al. Endothelins and their receptors in embryo implantation. J Cell Biochem. 2019; 120(9): 14274-84. https://doi.org/10.1002/jcb.28983.
Mubarak HJ, Ahmed SA. Histomorphometric and endothelin-1 immunohistochemical study of human placental villi correlated with umbilical cord coiling index. Iraqi JMS. 2017; 15 (2): 198-205.
Khidhir SM, Mahmud AM, Maulood IM. Association of Endothelin-I and A symmetric Dimethylarginine Levels with Insulin Resistance in Type-2 Diabetes Mellitus Patients. Baghdad Sci J. 2022; 19(1): 0055. http://dx.doi.org/10.21123/bsj.2022.19.1.0055.
Farhan TM. Endothelin-1 Expression in Placental Tissue Vasculature in Normal Vaginal Delivery and Cesarean Sections. Muthanna Med J. 2019; 6(1): 26-36.
Arinami T, Ishikawa M, Inoue A, Yanagisawa M, Masaki T, Yoshida MC, et al. Chromosomal assignments of the human endothelin family genes: the endothelin-1 gene (EDN1) to 6p23-p24, the endothelin-2 gene (EDN2) to 1p34, and the endothelin-3 gene (EDN3) to 20q13. 2-q13. 3. Am J Hum Genet. 1991; 48(5): 990.
National Center for Biotechnology Information. National Library of Medicine. EDN1 endothelin 1 - Homo sapiens (human).. (Accessed: 01 March 2023).
Mashkina EV, Kovalenko KA, Miktadova AV, Shkurat MA. Association of Gene Polymorphisms of Antioxidants with Reproductive Losses. Russ J Genet. 2020; 56: 354-62. https://doi.org/10.1186/1741-7015-11-154.
Flores JA. Gene expression of endothelin-1 in the porcine ovary: follicular development. Biol Reprod. 2000; 63(5): 1377-82. https://doi.org/10.1093/biolreprod/63.5.1377.
Apa R, Miceli F, Feo DD, Pierro E, Ayala G, Mancuso S, et al. Endothelin‐1: expression and role in human corpus luteum. Am J Reprod Immunol. 1998; 40(5): 370-6. https://doi.org/10.1111/j.1600-0897.1998.tb00068.x.
Riley SC, Findlay JK, Salamonsen LA. Endothelin-1 and endothelin receptors are present in the sheep uterus and conceptus at implantation. J Endocrinol. 1995; 147(2): 235-44. https://doi.org/10.1677/joe.0.1470235.
Inoue A, Yanagisawa M, Takuwa Y, Mitsui Y, Kobayashi M, Masaki T. The human preproendothelin-1 gene: complete nucleotide sequence and regulation of expression. J Biol Chem. 1989; 264(25): 14954-9. https://doi.org/10.1016/S0021-9258(18)63795-0.
Bulun SE. Steroids, cytokines, and implantation. Endocrinology. 2017; 158(6): 1575-6. https://doi.org/10.1210/en.2017-00407.
Telford JL, Macchia G, Massone A, Carinci V, Palla E, Melli M. The murine interleukin 1β gene: structure and evolution. Nucleic Acids Res. 1986; 14(24): 9955-63. https://doi.org/10.1093/nar/14.24.9955.
National Center for Biotechnology Information. National Library of Medicine. IL1B interleukin 1 beta- Homo sapiens (human). (Accessed: 01 March 2023).
Fontana VA, Sanchez M, Cebral E, Calvo JC. Interleukin-1β regulates metalloproteinase activity and leptin secretion in a cytotrophoblast model. Biocell. 2010 34(1): 37-43. https://doi.org/10.32604/BIOCELL.2010.34.037
Rébé C, Ghiringhelli F. Interleukin-1β and cancer. Cancers. 2020; 12(7): 1791. https://doi.org/10.3390/cancers12071791.
Sharma S, Godbole G, Modi D. Decidual control of trophoblast invasion. Am J Reprod Immunol. 2016; 75(3): 34150. https://doi.org/10.1111/aji.12466.
Schmittgen TD, Livak KJ. Analyzing real-time PCR data by the comparative CT method. Nat Protoc. 2008; 3(6): 1101-8. https://doi.org/10.1038/nprot.2008.73
Bińkowski J, Miks S. Gene-Calc [Computer software]. 2018. may, 2023.
Mastrogiannis DS, O'Brien WF, Krammer J, Benoit R. Potential role of endothelin-1 in normal and hypertensive pregnancies. J Obstet Gynaecol Res. 1991; 165(6) :1711-6. https://doi.org/10.1016/0002-9378(91)90020-R
Merviel P, Lourdel E, Cabry R, Boulard V, Brzakowski M, Demailly P, et al. Physiopathology of human embryonic implantation: clinical incidences. Folia Histochem. Cytobiol. 2009; 47(5): 25-34. https://doi.org/10.2478/v10042-009-0058-3
Yang H, Pang H, Miao C. Ovarian IL-1α and IL-1β levels are associated with primary ovarian insufficiency. Int J Clin Exp Path. 2018; 11(9): 4711.
Zhu X, Niu Z, Ye Y, Xia L, Chen Q, Feng Y. Endometrium cytokine profiles are altered following ovarian stimulation but almost not in subsequent hormone replacement cycles. Cytokine. 2019; 114: 6-10. https://doi.org/10.1016/j.cyto.2018.11.002.
Liang PY, Diao LH, Huang CY, Lian RC, Chen X, Li GG, et al. The pro-inflammatory and anti-inflammatory cytokine profile in peripheral blood of women with recurrent implantation failure. Reprod Biomed Online. 2015; 31(6): 823-6. https://doi.org/10.1016/j.rbmo.2015.08.009.
Boomsma CM, Kavelaars A, Eijkemans MJ, Lentjes EG, Fauser BC, Heijnen CJ, et al. Endometrial secretion analysis identifies a cytokine profile predictive of pregnancy in IVF. Hum Reprod. 2009; 24(6): 1427-35. https://doi.org/10.1093/humrep/dep011.
Rikimaru T, Awano S, Mineoka T, Yoshida A, Ansai T, Takehara T. Relationship between endothelin-1 and interleukin-1β in inflamed periodontal tissues. Biomed Res. 2009; 30(6): 349-55. https://doi.org/10.2220/biomedres.30.349.
Lin Z, Kubota T, Masuda M, Aso T. Role of nitric oxide synthase in release of endothelin from cultured human endometrial cells. Eur J Endocrinol. 1998; 138(4): 467-71. https://doi.org/10.1530/eje.0.1380467.
Downloads
Issue
Section
License
Copyright (c) 2024 Zainab Jalil Abdulkareem, Asmaa M. Salih Almohaidi
This work is licensed under a Creative Commons Attribution 4.0 International License.
How to Cite
