DNA barcoding of seven cyprinid fish species in the Iraqi Inland waters using mitochondrial COI gene sequence


  • Mustafa Sami Faddagh Ziyadi Department of Marine Vertebrate, Marine Science Centre, University of Basrah, Basra, Iraq. https://orcid.org/0000-0002-1609-0544




Cytochrome C Oxidase, Cyprinidae, DNA barcode, Iraq, Luciobarbus


Family Cyprinidae is the largest fish family in the Iraqi inland waters. The cyprinid fish species were described by traditional biometry. Family Cyprinidae fish species in Iraq are important because of ecological and economic aspects. While the morphological similarity among the cyprinid species made the identification not easy.  DNA barcoding was chosen to confirm the taxonomy and ensure genetic diversity. Seven cyprinid fish species, Luciobarbus barbulus, L. xanthopterus, L. kersin, L. esocinus, Arabibarbus grypus, Cyprinus carpio, and Acanthobrama marmaid were collected from the Shatt Al-Arab River, the Marshes, and the Mosul Dam reservoir. The mitochondrial Cytochrome C Oxidase gene of the specimens was amplified and sequenced. Universal primers were chosen for this purpose. Chromas software was used for processing the sequences. The result showed that the sequence ranged from 600-657 bp. While the neighbor-joining tree created by Clustal Omega software revealed the four Luciobarbus species clustering into two central branches, while the other three diverged. Nucleotide distribution statistically for the studied fish species was compared. The results of DNA barcoding using COI gene sequence proved the four independent Luciobarbus fish species. The COI gene sequence was successful as a DNA barcode which is accurate in species identification. The sequences were deposited in the gene bank under OM669701, OM669699, OM669702, OM669705, OM669700, OM669703 and OM669704. This study represents the starting line for the DNA barcoding project to detect all fish fauna sequences in the Iraqi inland waters. In addition, it will be very useful in the conservation program of native species in Iraqi inland waters.


Khalaf KT. The Marine and freshwater fishes of Iraq. Baghdad: Ar-Rabitta Press; 1961; 164 pp.

Martinez-Brown JM, Navarro-Flores J, Garcia-Rodriguez FJ, Ibarra-Castro L, Vargas-Peralta CE, Rio-Portilla M, et al. Revision of the diagnostic characters of two morphologically similar snook species, Centropomus viridis and C. nigreescens (Carangiformes: Centropomidae). Zootaxa 2021; 4915(3): 4915.3.2. https://doi.org/10.11646/zootaxa.4915.3.2.

Rao G, Krishna M, Sujatha K. Phylogenetic Relations and Electrophoretic Identification of Allozyme in Four Species of Snappers. Turk. J. Fish Aquat. Sci. 2017; 17: 1099-1106. https://doi.org/10.4194/1303-2712-v17_6_03

Vreven E, Adepo-Gourene B, Agnese J, Teugels G. Morphometric and allozyme variation in natural populations and cultured strains of the Nile tilapia Oreochromis niloticus (Teleostei; Cichlidae). Belg J Zool. 1998; 128(1): 23-34.

Hebert P, Cywinska A, Ball S, deWaard J. Biological identification through DNA barcodes. Proc Biol Sci. 2003; 270(1512): 313-321. https://doi.org/10.1098/rspb.2002.2218

Purty R and Chatterjee S. DNA barcoding: an effective technique in molecular taxonomy. Austin J Biotechnol. Bioeng. 2016; 3(1): 1059.

National Center for Biotechnology Information; 2023.

Hulley E, Taylor N, Zarnke A, Somers C, Manzon R, Wilson Y, et al. DNA barcoding vs. morphological identification of larval fish and embryos in Lake Huron: Advantages to a molecular approach. J Great Lakes Res. 2018 44; (5): 1110-1116. https://doi.org/10.1016/j.jglr.2018.07.013

Tang Q, Deng L, Luo Q, Duan Q, Wang X, Zhang R. DNA barcoding of fish species diversity in Guizhou, China. 2023; 15(2): 203. https://doi.org/10.3390/d15020203

Chen W, Ma X, Shen Y, Mao Y, He S. The fish diversity in the upper reaches of Salween River revealed by DNA barcoding. Sci Rep. 2015; 5: 17437. https://doi.org/10.1038/srep17437

Faddagh MS, Husain N, Al-Badran A. DNA fingerprinting of eight cyprinid fish species of Iraqi inland waters using RAPD-PCR. Adv Life Sci. 2012; 2(2): 9-16. https://doi.org/10.5923/j.als.20120202.03

Faddagh MS, Husain N, Al-Badran A Usage mitochondrial 16S rRNA gene as molecular marker in taxonomy of cyprinin fish species (Cyprinidae: Teleostei). JKAU: Mar Sci. 2012; 23(1): 39-49. https://doi.org/10.4197/Mar.23-1.3

Coad B. Freshwater fishes of Iraq. Pensoft Publishers, Bulgaria 2010; 231pp.

Ivanova N and Grainger C Primer sets for DNA barcoding. Canadian Centre for DNA Barcoding (CCDB) 2007; 2pp.

Vu Q, Truong O, Linh T, Dang B. Molecular phylogeny of Cyprinidae and Cobitidae (Teleostei; Cypriniformes) implication for Mekong fish. Proc. ISER 169 Internat. Conf Bangkok Thai. 21-22 Nov. 2018.

Hubert N, Kadarusman Ph, Wibowo A, Busson F, Caruso D, Sulandari S, et al. DNA barcoding of Indonesian freshwater fishes: challenges and prospects. DNA barcodes. 2015; 3(1): 144-169. https://doi.org/10.1515/dna-2015-0018

Bingpeng X, Heshan L, Zhan Z, Chunguang W, Yanguo W, and Hianjun W. DNA barcoding for identification of fish species in the Taiwan Strait. PLoS One. 2018; 13(6): e0198109.


Pegg C, Sinclair B, Briskey L, Aspden W. MtDNA barcode identification of fish larvae in Southern Great Barrier Reef, Australia. Sci Mar. 2006; 70: 7-12. https://doi.org/10.3989/scimar.2006.70s27

Geiger M, Herder F, Monaghan M, Almada V, Barbieri R, Bariche M, et al. Spatial heterogeneity in the Mediterranean Biodiversity Hotspot affects barcoding accuracy of its freshwater fishes. Mol Ecol Resour 2014;14(6):1210-1221. https://doi.org/10.1111/1755-0998.12257

Ali F, Ismail M, Aly W. DNA barcoding to characterize biodiversity of freshwater fishes of Egypt. Mol Biol Rep. 2020; 47(8): 5865-5877. https://doi.org/10.1007/s11033-020-05657-3.





How to Cite

DNA barcoding of seven cyprinid fish species in the Iraqi Inland waters using mitochondrial COI gene sequence. Baghdad Sci.J [Internet]. [cited 2024 Jul. 22];22(1). Available from: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/9802