إمكانات المعالجة البيولوجية باستخدام طحلب Chlorella vulgaris وNostoc paludosum على أصباغ الآزو مع تحليل التغيرات الأيضية
الشريط الجانبي للمقالة
محتوى المقالة الرئيسي
الملخص
استخدمت الطحالب المجهرية على نطاق واسع في عملية المعالجة البيولوجية لتحلل أو تكثيف الأصباغ السامة. شملت الدراسه الحاليه تقييم كفاءة كلا من طحلب C. vulgaris و N. paluodosum فى إزالة اللون الخاص باثنين من الأصباغ السامة ; هما صبغه الكريستال البنفسجي (CV) و الملكيت الأخضر (MG) علاوة على ذلك فقد تم تحديد ملامح التمثيل الغذائي للنوعين. أيضا متابعة تأثيرالأصباغ على النمط الأيضي للطحالب التي تمت دراستها. أظهرت البيانات أن طحلب C. vulgaris كان أكثر فاعلية في إزالة تلوين MG و CV ، وكانت أعلى نسبة إزالة للون هى 93.55 ٪ في حالة MG ، بينما سجلت نسبة 62.98 ٪ لإزالة لونCV . اما فى حالة طحلب N. paluodosum كانت النسبة المئوية لإزالة لون MG هى 77.6 ٪ ، ونسبة إزالة اللون من CV كانت 35.1 ٪. تم عمل النمط الأيضي للطحلبين باستخدام التحليل الطيفي للرنين المغناطيسي النووي (NMR) استنادًا إلى بيانات 1 D و 2D وتم تحديد 43 مركبًا في مستخلص طحلب C. vulgaris ، بينما تم تحديد 34 مركبا فى حالة طحلب N. paluodosum وشملت المركبات التي تم تحديدها الكربوهيدرات والأحماض الأمينية والأحماض العضوية و البيبتيدات الثنائية والفينولات. تم إجراء تحليلات إحصائية للتعرف على نمط تباين الأيض بين عينات مجموعه السيطره والطحالب المعالجة بالأصباغ. وقد أوضح تحليل المكون الرئيسى والتحليل العنقودي الهرمي أن العينات التي تمت معاملتها باستخدام MG منفصلة بوضوح عن عينة السيطره في كلا النوعين من الطحالب. بناءً على بيانات خريطة الحرارة يتأثر مستوى تركيز الكربوهيدرات والأحماض الأمينية بشدة بالمعالجة الحيوية لصبغ MG مقارنة بصبغة CV..
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