دور الإجهاد الملحي في الزراعة المختبرية للطحلب الأخضر انتيرومورفا كومبريسا وفعاليته المضادة للأكسدة
الشريط الجانبي للمقالة
محتوى المقالة الرئيسي
الملخص
تمت زراعة العشب البحري الاخضر انتيرومورفا كومبريسا (المثبت علي دعامته الصخرية الصغيرة) معمليا تحت الظروف الطبيعية لفصل الربيع من درجة حرارة و شدة اضاءه و توقيت ضضوي. و ذلك لدراسة مدي تحمل هذا العشب الاخضر و النامي طبيعيا في منطقة المد و الجزر، للاجهاد الملحي (تركيزات أقل و أكبر من تركيز ماء البحر). و لهذا تمت زراعتة معمليا و نقله بعد جمعه من بيئته و هو مثبت علي دعامتة الصخرية الصغيرة و في فصل الربيع (حيث يسود نموه في منطقة المد و الجزر) و أقلمته لمدة اسبوع علي النمو معمليا تحت نفس ظروف بيئته ثم نموه تحت ظروف اجهاد ملحي صناعي (ASW) و مقارنته بعينه ضابطه تنمو تحت ظروف ماء بحر طبيعي (NSW) و باستخدام تهويه بمعدل ثابت خلال مده التجربة و تحت ظروف فصل الربيع الطبيعية من ضوء و درجة حرارة.
تم تعيين معدل النمو للطحلب (ممثل بالمحتوي الصبغي الكلي للكلوروفيل و الكاروتينويدات) علي فترات منتظمة (كل 5 أيام) خلال مدة التجربة (25 يوما). تم عمل مستخلصات للطحلب النامي تحت ظروف الاجهاد الملحي المختلفة باستخدام مذيبات عضوية مختلفه القطبيه(petroleum ether, chloroform, ethyl acetate and acetone) و تعيين المحتوى الصبغي و مضادات الاكسده (مقارنه بمضاد الاكسده القياسي BHT). أظهرت النتائج وجود زياده في معدل النمو خاصة عند تركيزات 35, 53 g/L و بمعدل أقل عند تركيز106 g/L و ذلك خلال الخمسه عشر يوما الاولي للتجربة ثم بدأ بعدها تدهور ملحوظ و تدريجي للنمو نتيجة تأثير الاجهاد الملحي التراكمي علي عمليتي البناء الضوئي و التمثيل الغذائي.
بالنسبة للنشاط المضاد للاكسده و الذي يعتمد علي التركيز، نوع المذيب العضوي للمستخلص و علي مدة التحضين ، فقد تم تعيينه بعد الخمسه عشر يوما الاولي للتجربة و في نهايتها (25 يوم) ، أظهر مستخلص الاسيتون أعلي نشاط مضاد للاكسدة تحت كل الظروف و التركيزات مقارنة بباقي المستخلصات و بمضاد الاكسدة القياسي BHT، و كان أكثرها نشاط هو أكبر تركيز للملوحة (106 g/L).
تفاصيل المقالة
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