تقييم التأثيرات السمية الخلوية لمركب Colchicine المعزول من أوراق نبات (Calotropis procera (Ait ضد خطوط الخلايا السرطانية MCF-7 و SK-GT-4.
DOI:
https://doi.org/10.21123/bsj.2023.7091الكلمات المفتاحية:
الفعالية الضد سرطانية،اوراق نبات Calotropis procera،استخلاص وتنقية Colchicine، GC-M،HPLCالملخص
تعتبر القلويدات من المركبات الكيميائية المهمة الحاوية على النايتروجين والتي تعتبرمصدر غني لاكتشاف وتطوير عقاقير جديدة حيث تمتلك معظم القلويدات ذات الاصل النباتي تأثيرمضاد للسرطان. تم تحديد وجود القلويدات في اوراق نبات Calotropis procera باستخدام نوعين من الكواشف الكيميائية للقلويدات.تم اجراء تحليل GC-MS للمستخلص القلويدي لأوراق هذا النبات حيث بينت النتائج ظهور مركب قلويدي واحد عند وقت احتجاز بلغ 12.8دقيقة وبوزن جزيئي مقداره 399 وبتركيز 1.7% الذي تم تشخيصه كمركب الكولجسين ((C22H25N06 وذلك بالاعتماد على وجود المركب المعياري للكولجسين .كذلك تم فصل وتشخيص وتنقية مركب الكولجسين وذلك باستخدام تقنية كروماتوغرافي السائل عالي الاداء HPLC لمستخلص القلويدات للأوراق حيث اظهرت النتائج وجود مركب الكولجسين عند وقت احتجاز2.5 دقيقة وذلك بمطابقته مع المركب المعياري له. تم تقييم الفعالية المضادة للسرطان لمركب الكولجسين المنقى على نوعين من خطوط الخلايا السرطانية MCF-7 وSK-GT-4 حيث بينت النتائج وجود فروق معنوية للفعالية السمية لمركب الكولجسين ضد الخط الخلوي MCF-7 على تلك التي ضد الخط الخلوي SK-GT-4 كذلك تم احتساب التركيز نصف المثبط IC50 لكلا الخطين والتي بلغت55.33و522مايكروغرام|مل على الترتيب.
Received 22/2/2022
Revised 10/8/2022
Accepted 11/8/2022
Published Online First 20/2/2023
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