التعبير الجيني ومستويات المثيلة لجين PCSK9 للمرضى العراقيين المصابين بتصلب الشريان التاجي
محتوى المقالة الرئيسي
الملخص
يرتبط التعبير الجيني لجين PCSK9ارتباطًا وثيقًا بمستويات الدهون وخطر الإصابة بمرض تصلب الشرايين التاجية. تهدف الدراسة الحالية إلى قياس كمية التعبير الجيني للجين PCSK9 وتأثير المثيلة على مستوى تعبيره الذي يساهم في التسبب في اضطراب الشريان التاجي الحاد.
تضمنت الدراسة الحالية 150 شخصًا من المجتمع العراقي ، 100 مريض بمرض الشريان التاجي و 50 من الأصحاء. تم تحديد تركيز PCSK9 في كل عينات المصل المدروسة بواسطة تقنية ELISA ، وتم تقدير مستويات التعبير عن جين PCSK9 في الدم الكامل بطريقة qRT-PCR ، واكتشاف مستوى مثيلة الحمض النووي في جين PCSK9 باستخدام تقنية HRM-PCR.
كان التعبير عن جين ب مستوى اعلى PCSK9 6.06 ± 1.84 في مجموعة مرضى ASCAD مقارنة بمجموعة التحكم 1.00 ± 0.19. أيضا كان تركيز بروتين PCSK9 عالياً في مجموعة المرضى مقارنة بمجموعة الاصحاء P = <0.01. فضلا عن ان مستويات مثيلة PCSK9 ، اظهرت فرقاً معنوياً بين مجموعتي الدراسة بمستوى 0.002P=. في الختام ، ترتبط مستويات بروتين PCSK9 في المصل بمرض الشريان التاجي كما موضح في receiver operating characteristic (ROC) analysis ، مما يؤدي إلى اعتبار أن تركيز بروتين PCSK9 قد يكون مؤشرًا جيدًا لتطور مرض الشريان التاجي.
Received 8/9/2022
Revised 22/11/2022
Accepted 24/11/2022
Published Online First 20/4/2023
تفاصيل المقالة
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كيفية الاقتباس
المراجع
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