تعدد الطرز الوراثية لمورث الإنتجرين ألفا 2 ومورث الإنتجرين بيتا 3 كعوامل خطر للعقم عند الإناث العراقيات العقيمات الخاضعات لبرنامج الاخصاب خارج الجسم

المؤلفون

  • أسماء محمد صالح المهيدي قسم علوم الحياة ، كلية العلوم للبنات، جامعة بغداد، بغداد، العراق.
  • رباب عبد الرضا يوسف مدرسة المناقب المتوسطة للبنات، وزارة التربية، بغداد، العراق.

DOI:

https://doi.org/10.21123/bsj.2024.8217

الكلمات المفتاحية:

تحليل عالي الدقة ، الانغراس ، جين الانتجرين الفا 2 ، جين الانتجرين بيتا 3 ، الاخصاب خارج الجسم .

الملخص

صُممت الدراسة الحالية للتحري عن تأثير تعدد الطرز أحادي النوكليوتيدات (SNP) ، للتتابع المرجعي  5918 ،(T> C) لمورث إنتيجرين بيتا 3 (ITGB3) وللتابع المرجعي 1801106    (G> A) إنتجرين ألفا 2 (ITGB3) في عينات دم اناث عراقيات. 71 أنثى مصابة بالعقم خاضعة لبروتوكول أطفال الأنابيب (مقسمة إلى مجموعتين فرعيتين ، 29 عملية زرع ناجحة و 42 عملية زرع فاشلة) و 50 أنثى خصبة كمجموعة سيطرة. تم الحصول على عينات الدم من المرضى ومجموعات السيطرة في المستشفيات العامة في بغداد ، العراق من مارس 2021 إلى أبريل 2021. تم إجراء التنميط الوراثي لتعدد الطرز باستخدام تحليل الذوبان عالي الدقة في الوقت الحقيقي (HRM في الوقت الحقيقي PCR) من الحمض النووي الرايبوزي منقوص الاوكسجين الجينومي المنقى المأخوذ من عينات الدم. أظهرت النتائج أن الطرز الوراثية TT و GG لـلتتابعات المرجعية 5918 في ITGB3 و 1801106  في ITGA2 على التوالي ، كانت أعلى بشكل ملحوظ في الإناث الخصبة من الاناث العقيمات. يشير ذلك إلى أن الطراز الوراثي  GG يعمل كعامل وقائي ، بينما يعمل الطراز الوراثي TC لـلتتابع المرجعي 5918 في ITGB3 و AA من الطراز الوراثي للتتابع المرجعي 1801106 في ITGA2 كعوامل خطر. الاستنتاج ، ان لتعدد الطرز في جينات ITGA2 و ITGB3 تأثير على استعداد مجموعة من الاناث ضمن الدر اسة  للإصابة بالعقم.

المراجع

Sun Y, Zhang Y. Ma X, Jia W , Su Y. Determining diagnostic criteria of unexplained recurrent implantation failure: A retrospective study of the two vs three or more implantation failure. Front. Endocrinol. 2021; 12: 851. https://doi.org/10.3389/fendo.2021.619437.

Vander Borght M, Wyns C. Fertility and infertility: Definition and epidemiology. Clin Biochem. 2018; 62: 2-10.‏ https://doi.org/10.1016/j.clinbiochem.2018.03.012 .

Akhondi M M, Ranjbar F, Shirzad M, Ardakani Z B, Kamali, K , Mohammad K. Practical difficulties in estimating the prevalence of primary infertility in Iran. Int J Fertil Steril. 2019; 13(2): 113.‏ https://doi.org/10.22074%2Fijfs.2019.5583.

World Health Organization. Infertility.: http//www.who.int /2021.

World Health Reorganization. Iraqi Family Health Survey report. http//www.who.int /2008.

Hassab, Z S. Almohaidi A M S. Alsaidi F S. Association between the Genetic Variation of LIF R Gene with Its Gene Expression and Implantation Outcomes of IVF-Iraqi Arab Females. J Pharm Negat Results. 2022; 13( 4). https://doi.org/ 10.47750/pnr.2022.13.04.230.

Thabit, M A. Abdullah G H. AL-Rawi K F. Polymorphism study of MTHFR 677C? T and its correlation with oxidative stress and their influence on female infertility in Erbil–Iraq. Baghdad Sci J. 2017; 14(3): 0611-0611.‏ http://dx.doi.org/10.21123/bsj.2017.14.3.0611.

Centers for Disease Control and Prevention. Infertility FAQs. (2013). https://www.cdc.gov/reproductivehealth/infertility/index.htm.

Mitri F, Nayot D, Casper R F, Bentov Y. Current tools for the optimization of embryo transfer technique for recurrent implantation failure. Minerva Ginecol, 2016; 68(4): 431-449.

Ochoa-Bernal M A, Fazleabas A T . Physiologic events of embryo implantation and decidualization in human and non-human primates. Int. J Mol Sci. 2020; 21(6): 1973.‏ https://doi.org/10.3390/ijms21061973.

Raheem K A. Cytokines, growth factors and macromolecules as mediators of implantation in mammalian species. Int J Vet Sci Med. 2018; 6: S6-S14.

Jain M , Chauhan AK. Role of integrins in modulating smooth muscle cell plasticity and vascular remodeling: from expression to therapeutic implications. Cells. 2022; 11(4): 646. https://doi.org/10.3390/cells11040646

Mezu-Ndubuisi O J, Maheshwari A. The role of integrins in inflammation and angiogenesis. Pediatr Res. 2021; 89(7): 1619–1626. https://doi.org/10.1038/s41390-020-01177-9.

Busman H , Farisi S. Regulation of Integrin β3 Protein Secretion on Implantation Embryo of Mouse (Mus musculus L.) Induced by Oil Atsiri of Purple Nutsedge Tubers (Cyperus rotundus L.). Annu Res Rev Biol. 2019; 1-5.‏ https://doi.org/10.9734/arrb/2019/v33i230119

Zhou L , Li C, Liu X, Zhang T. Effect of Irisin on LIF and integrin αvβ3 in rats of implantation failure. Reprod Biol Endocrinol. 2021; 19(1): 1-7.‏ https://doi.org/10.1186/s12958-021-00700-9

Lessey B A. Endometrial integrins and the establishment of uterine receptivity. Hum. Reprod. 1998; 13: 247–258. https://doi.org/10.1093/humrep/13.suppl_3.247

Adorno-Cruz V, Liu H. Regulation and Functions of Integrin Alpha2 in Cell Adhesion and Disease. Genes Dis . 2019; 6(1): 16–24.https://doi.org/10.1016/j.gendis.2018.12.003

Simon M, Kouskouni E, Vitoratos N, Economou E, Creatsas G. Polymorphisms of platelet glycoprotein receptors and cell adhesion molecules in fetuses with fetal growth restriction and their mothers as detected with pyrosequencing. In Viv. 2017; 31: 243–50. https://doi.org/10.21873/invivo.11052

Al-Mishrey M K. Evaluation of Beta-2 Integrin and Platelets Roles in Sickle Cell Disease Pathogenicity in Basrah Governorate Patients. Baghdad Sci J. 2022; 20(1): 7-12 . http://dx.doi.org/10.21123/bsj.2022.6601.

Al-Qaisi R M, Al-Ani L , Al-Halbosiy M M. Genetic Polymorphism of ITGA2 Gene and the Risk of Heart Attack and Stroke in Al-Anbar Population/Iraq. J Pharm. Sci Res .2018; 10(9): 2305-2308.

Magazova A, Ashirbekov Y, Abaildayev A, Satken K, Balmukhanova A, Akanov Z, et al. ITGA2 Gene Polymorphism Is Associated with Type 2 Diabetes Mellitus in the Kazakhstan Population. Medicina, 2022; 58(10): 1416. https://doi.org/10.3390/medicina58101416

Database G H. ITGB3 Gene - GeneCards | ITB3 Protein | ITB3 Antibody. 2019.

Huang J, Li X, Shi X, Zhu M, Wang J, Huang S, Jin J. Platelet integrin αIIbβ3: signal transduction, regulation, and its therapeutic targeting. J hematol oncol. 2019; 12(26): 1-22.‏ https://doi.org/10.1186/s13045-019-0709-6.

Di Micco, P., Russo, V., Mastroiacovo, D., Bosevski, M., & Lodigiani, C. In vitro fertilization procedures with embryo transfer and their association with thrombophilia, thrombosis and early antithrombotic treatments. J. Blood Med. 2020 ; (11): 185-190.‏ https://doi.org/10.2147/JBM.S248988.

Yousif R A, Almohaidi A S , Al-Musawi B J. Assessment of ITGB3 gene expression and hormonal status in infertile female undergoing an in vitro fertilization protocol. HIV Nursing. 2023; 23(1): 770-778.‏ https://doi.org/10.31838/hiv23.01.130.

Yousif R A, Almohaidi A S. Identification of ITGB3 Polymorphisms with implantation outcome during in vitro fertilization program. HIV Nursing. 2023; 23(1): 257-264. https://doi.org/10.31838/hiv23.01.42.

Langsenlehner U, Renner W, Yazdani-Biuki B, Eder T , Wascher T C , Paulweber B. Integrin alpha-2 and beta-3 gene polymorphisms and breast cancer risk. Breast Cancer Res Treat. 2006; 97(1): 67-72.‏ https://doi.org/10.1007/s10549-005-9089-4

Newman P J, Derbes R S, Aster R H. The human platelet alloantigens, PlA1 and PlA2, are associated with a leucine33/proline33 amino acid polymorphism in membrane glycoprotein IIIa, and are distinguishable by DNA typing. J clin invest. 1989; 83(5): 1778-1781.‏ https://doi.org/10.1172/JCI114082.

Buitrago L, Lefkowitz S, Bentur O, Padovan J, Coller B. Platelet binding to polymerizing fibrin is avidity driven and requires activated αIIbβ3 but not fibrin cross-linking. Blood Adv. 2021; 5(20):3986-4002.‏ https:// https://doi.org/10.1182/bloodadvances.2021005142.

Rotimi C, Leppert M, Matsuda I, Zeng C, Zhang H, Adebamowo C, et al. Community engagement and informed consent in the International HapMap project. Public Health Genom.2007 10(3): 186-198.‏ https://doi.org/10.1159/000101761.

Xiang Q, Ji SD, Zhang Z, Zhao X, Cui YM. Identification of ITGA2B and ITGB3 single-nucleotide polymorphisms and their influences on the platelet function. Biomed Res Int. 2016; 14 .https://doi.org/10.1155/2016/5675084

Komsa-Penkova R , Golemanov G, Tsankov B , Ivanov P , Beshev L , Tonchev P. Rs5918ITGB3 polymorphism, smoking, and BMI as risk factors for early onset and recurrence of DVT in young women. Clin Appl Thromb Hemost. 2017; 23(6): 585-595. https://doi.org/10.1177/1076029615624778

Oros M M, Lutz V, Pavlo A, Sitkar A. Investigation of the influence of thrombophilic genes polymorphism, including serpin 1 (pai-i), fii, prothrombin and ITGB3-β integrin, on the frequency of stroke in association with controllable risk factors for its occurrence. Wiad Lek. 2020; 73(3): 471-477.‏ https://doi.org/10.36740/WLek202003112

Yang W, Sun Q, Zhou Z, Gao Y, Shi F, Wu X, Kang, X. Coagulation parameters predictive of repeated implantation failure in Chinese women: A retrospective study. Medicine, . 2020; 99(48). https://doi.org/10.1097/MD.0000000000023320

.‏Karami F, Askari M, Modarressi M . Investigating association of rs5918 human platelets antigen 1 and rs1800790 fibrinogen β chain as critical players with recurrent pregnancy loss. Med Sci. 2018; 6(4): 98.‏ https://doi.org/10.3390/medsci6040098

Chatzidimitriou M, Chatzidimitriou D, Mavridou M, Anetakis C, Chatzopoulou F, Lialiaris T. Thrombophilic gene polymorphisms and recurrent pregnancy loss in Greek women. Int J Lab Hematol. 2017; 39(6): 590-595.‏ https://doi.org/10.1111/ijlh.12703

Bigdeli R, Younesi M R, Panahnejad E, Asgary V, Heidarzadeh S, Mazaheri H, et al. Association between thrombophilia gene polymorphisms and recurrent pregnancy loss risk in the Iranian population. Syst Biol Reprod Med. 2018; 64(4): 274-282.‏ https://doi.org/10.1080/19396368.2018.1456576

Al-Astal M, Sharif F. Beta-fibrinogen (-455 G/A) and Integrin beta-3 (PLA1/A2) polymorphisms and recurrent pregnancy loss in Gaza strip-Palestine. Int J Reprod Contracept Obstet Gynecol. 2014; 3(1): 134-139.‏ https://doi.org/10.1242/dev.162305.

Li Z, Li R, Li X, Dai H, Han X, Wang X, Yang A. LIF in embryo culture medium is a predictive marker for clinical pregnancy following IVF-ET of patients with fallopian tube obstruction. J Reprod Immunol. 2020; 141: 103164.‏ https://doi.10.1016/j.jri.2020.103164.

Kunicki T J, Williams S A, Diaz D, Farndale RW, Nugent D J. Platelet adhesion to decorin but not collagen I correlates with the integrin α2 dimorphism E534K, the basis of the human platelet alloantigen (HPA)-5 system. Haematologica. 2012; 97(5): 692.‏695. https://doi.org/10.3324%2Fhaematol.2011.056556

Kydonopoulou K, Rousso D, Ilonidis G, Mandala E. Association of GPIa C807T polymorphism with unexplained female infertility and IVF implantation failure. J Biol Regul Homeost Agents. 2019; 33(3): 883-887.‏

Lee C Q, Turco M Y, Gardner L, Simons B D, Hemberger M, Moffett A. Integrin α2 marks a niche of trophoblast progenitor cells in first trimester human placenta. Development. 2018; 145(16): dev162305.‏ https://doi.org/10.1242/dev.162305

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كيفية الاقتباس

1.
تعدد الطرز الوراثية لمورث الإنتجرين ألفا 2 ومورث الإنتجرين بيتا 3 كعوامل خطر للعقم عند الإناث العراقيات العقيمات الخاضعات لبرنامج الاخصاب خارج الجسم. Baghdad Sci.J [انترنت]. [وثق 27 سبتمبر، 2024];22(4). موجود في: https://bsj.uobaghdad.edu.iq/index.php/BSJ/article/view/8217