تعدد الطرز الوراثية لمورث الإنتجرين ألفا 2 ومورث الإنتجرين بيتا 3 كعوامل خطر للعقم عند الإناث العراقيات العقيمات الخاضعات لبرنامج الاخصاب خارج الجسم
DOI:
https://doi.org/10.21123/bsj.2024.8217الكلمات المفتاحية:
تحليل عالي الدقة ، الانغراس ، جين الانتجرين الفا 2 ، جين الانتجرين بيتا 3 ، الاخصاب خارج الجسم .الملخص
صُممت الدراسة الحالية للتحري عن تأثير تعدد الطرز أحادي النوكليوتيدات (SNP) ، للتتابع المرجعي 5918 ،(T> C) لمورث إنتيجرين بيتا 3 (ITGB3) وللتابع المرجعي 1801106 (G> A) إنتجرين ألفا 2 (ITGB3) في عينات دم اناث عراقيات. 71 أنثى مصابة بالعقم خاضعة لبروتوكول أطفال الأنابيب (مقسمة إلى مجموعتين فرعيتين ، 29 عملية زرع ناجحة و 42 عملية زرع فاشلة) و 50 أنثى خصبة كمجموعة سيطرة. تم الحصول على عينات الدم من المرضى ومجموعات السيطرة في المستشفيات العامة في بغداد ، العراق من مارس 2021 إلى أبريل 2021. تم إجراء التنميط الوراثي لتعدد الطرز باستخدام تحليل الذوبان عالي الدقة في الوقت الحقيقي (HRM في الوقت الحقيقي PCR) من الحمض النووي الرايبوزي منقوص الاوكسجين الجينومي المنقى المأخوذ من عينات الدم. أظهرت النتائج أن الطرز الوراثية TT و GG لـلتتابعات المرجعية 5918 في ITGB3 و 1801106 في ITGA2 على التوالي ، كانت أعلى بشكل ملحوظ في الإناث الخصبة من الاناث العقيمات. يشير ذلك إلى أن الطراز الوراثي GG يعمل كعامل وقائي ، بينما يعمل الطراز الوراثي TC لـلتتابع المرجعي 5918 في ITGB3 و AA من الطراز الوراثي للتتابع المرجعي 1801106 في ITGA2 كعوامل خطر. الاستنتاج ، ان لتعدد الطرز في جينات ITGA2 و ITGB3 تأثير على استعداد مجموعة من الاناث ضمن الدر اسة للإصابة بالعقم.
Received 12/12/2022
Revised 03/11/2023
Accepted 05/11/2023
Published Online First 20/09/2024
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